Sm d18 1 18 0

Manufactured by Avanti Polar Lipids

SM d18:1/18:0 is a synthetic sphingomyelin lipid. Sphingomyelins are a class of sphingolipids that are important structural components of cell membranes. This specific molecule has a ceramide backbone with a stearic acid (18:0) fatty acid chain and a d18:1 sphingosine backbone.

Automatically generated - may contain errors

Lab products found in correlation

Cer d18 1 18 0, by Avanti Polar Lipids (2 mentions) Cer d18 1 15 0, by Avanti Polar Lipids (2 mentions) Pe 12 0 12 0, by Avanti Polar Lipids (2 mentions) Pc 13 0 13 0, by Avanti Polar Lipids (2 mentions) Pc 16 0 16 0, by Avanti Polar Lipids (2 mentions) Sm d18 1 12 0, by Avanti Polar Lipids (2 mentions) Polyalanine, by Merck Group (1 mentions) Hplc grade, by Thermo Fisher Scientific (1 mentions) Stigmasterol, by Avanti Polar Lipids (1 mentions) Ammonium formate, by Merck Group (1 mentions)

3 protocols using sm d18 1 18 0

Lipid Ester Standards for Mass Spectrometry

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Lipid ester standards (PC(17:0/20:4), PE(17:0/20:4), PA(17:0/20:4), PS(17:0/20:4), PI(17:0/20:4), and SM(d18:1/18:0)) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Solvents and chemicals used for extraction and mass spectrometry were HPLC grade, purchased from Fisher Scientific Company (Pittsburgh, PA), and were used without further purification. Hanks Balanced Salt Solution (HBSS) was purchased from Mediatech (Manassas, VA). Human serum, SRM 909C, was obtained from the National Institute of Standards and Technology (Gaithersburg MD). Polyalanine was obtained from Sigma (St. Louis MO).

Zemski Berry K.A., Barkley R.M., Berry J.J., Hankin J.A., Hoyes E., Brown J.M, & Murphy R.C. (2016). Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids. Analytical chemistry, 89(1), 916-921.

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Lipid Profiling Using HPLC-MS

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All reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA) unless specified. The liquid chromatography solvent was HPLC-grade and purchased from Acros Organics. Synthetic lipid standards (Cer d18:1/18:0, Cer d18:1/15:0, PE 12:0/12:0, PE 16:0/16:0, PC 13:0/13:0, PC 16:0/16:0, SM d18:1/18:0, SM d18:1/12:0, stigmasterol, cholesteryl heptadecanoate and glyceryl trinonadecanoate) were purchased from Avanti Polar Lipids and Sigma-Aldrich.

Chicanne G., Barrachina M.N., Durbec A., Bertrand-Michel J., Troitiño S., Hermida-Nogueira L., Sueiro A.M., Pardo M., Payrastre B, & García Á. (2022). Platelet Lipidome Fingerprint: New Assistance to Characterize Platelet Dysfunction in Obesity. International Journal of Molecular Sciences, 23(15), 8326.

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Lipidomic Analysis of Synthetic Standards

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The liquid chromatography solvent, acetonitrile, was HPLC-grade and purchased from Acros Organics. Ammonium formate (>99%) was supplied by Sigma Aldrich. Synthetic lipid standards (Cer d18:1/18:0, Cer d18:1/15:0, PE 12:0/12:0, PE 16:0/16:0, PC 13:0/13:0, PC 16:0/16:0, SM d18:1/18:0, SM d18:1/12:0) were purchased from Avanti Polar Lipids.

Régnier M., Polizzi A., Smati S., Lukowicz C., Fougerat A., Lippi Y., Fouché E., Lasserre F., Naylies C., Bétoulières C., Barquissau V., Mouisel E., Bertrand-Michel J., Batut A., Saati T.A., Canlet C., Tremblay-Franco M., Ellero-Simatos S., Langin D., Postic C., Wahli W., Loiseau N., Guillou H, & Montagner A. (2020). Hepatocyte-specific deletion of Pparα promotes NAFLD in the context of obesity. Scientific Reports, 10, 6489.

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