Human α2 antiplasmin

Recombinant human FXIII-A₂ was obtained from the late Dr. Paul Bishop (Zymogenetics, Seattle, WA), and recombinant human thrombin from E. Di Cera and L. Pelc (St. Louis University, MO). The activity of the recombinant human thrombin was determined using titration against hirudin.^{31 (link)} Human plasma fibronectin and Phe-Pro-Arg-chloromethyl ketone (PPACK) were supplied by Haematologic Technologies (Essex Junction, VT, USA). Bovine thrombin, human α₂-antiplasmin, actin, ferulic acid, α-cyano-4-hydroxycinnamic acid (α-CHCA), N,N-Dimethyl-p-phenylenediamine (DMPDA), monodansylcadaverine (MDC), and Glycine ethyl ester (GEE) hydrochloride were obtained from Millipore Sigma (St. Louis, MO, USA). Peptides α₂AP WT (1 – 15) and α₂AP Q4S (1 – 15) were synthesized by New England Peptide (Gardner, MA, USA). Peptide stock concentrations were determined by quantitative amino acid analysis (AAA Service Laboratory, Damascus, OR, USA). Sequencing grade chymotrypsin and GluC endoproteinase, both from Roche, were supplied by Millipore Sigma (St. Louis, MO, USA). 30% Acrylamide/Bis solution, 4 – 20 % precast tris-glycine gels, and dual-color molecular weight standards were purchased from Bio-Rad Laboratories (Richmond, CA, USA).