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2 protocols using l iso

1

Studying LCZ696 Effects in Rats

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Male Wistar rats weighing 187–211 g were purchased from Japan SLC (Shizuoka, Japan). Rats were housed at an animal facility in a 12-h lightdark cycle and were provided with standard rat chow and water ad libitum. LCZ696 and valsartan were kindly supplied by Novartis Pharma AG (Basel, Switzerland). LCZ696 includes molecular moieties of valsartan and sacubitril at a 1:1 ratio. L-ISO was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Biogenic Amine and Amino Acid Analysis

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BA standards (tyramine hydrochloride, 2-phenylethylamine hydrochloride, putrescine dihydrochloride, cadaverine dihydrochloride, histamine dihydrochloride, tryptamine hydrochloride, spermidine trihydrochloride) and amino acid standards (L-Asp, L-Glu, L-Ser, L-Gly, L-His, L-Thr, L-Arg, γ-aminobutyric acid [GABA], L-Ala, L-Pro, L-Tyr, L-Val, L-Met, L-Iso, L-Leu, L-Phe, L-Trp, and L-Lys) were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Dansyl chloride, perchloric acid, sodium hydrogen carbonate, potassium dichromate, and silver nitrate were purchased from Daejung Chemical Co. (Siheung, Korea). Distilled water, acetone, and acetonitrile (HPLC grade) were purchased from Tedia Co. (Fairfield, OH, USA).
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