Anti mica b

Manufactured by BD

Anti-MICA/B is a laboratory reagent used to detect the presence of MICA and MICB proteins in biological samples. It is a tool for research purposes only and its specific applications may vary depending on the research objectives.

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MICA/B (4 citations)

3 protocols using anti mica b

Characterization of Cancer Stem Cells

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Cell aliquots were stained with fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE–Cyanin 7 (PC7), or allophycocyanin (APC)-conjugated mouse mAbs against anti-HLA-ABC-FITC (BD Biosciences Pharmingen), anti-Sox-2-APC (Becton Dickinson BD Biosciences Italy, Pharmingen) and CIK-target antigens [anti-MIC A/B (BD Biosciences Pharmingen), anti-ULBPs, anti CD112 and anti CD155 (R&D System, Space Import Export)]. Intracellular expression of Oct4 was detected after fixation/permeabilization by the Cytoperm/Cytofix Kit according to the manufacturer’s instructions (BD Biosciences Pharmingen). ALDH activity was evaluated by ALDEFLUOR assay kit (Aldagen, Stemcell Technologies), according to manufacturer’s instructions. Stro-1 expression was detected by flow cytometric staining tumor cells with APC-conjugated anti-Stro-1 monoclonal antibody (Biolegend). Labeled cells were read on a FACS Cyan (Cyan ADP, Beckman Coulter s.r.l.) and analyzed using Summit Software.

Mesiano G., Grignani G., Fiorino E., Leuci V., Rotolo R., D’Ambrosio L., Salfi C., Gammaitoni L., Giraudo L., Pisacane A., Butera S., Pignochino Y., Basiricó M., Capozzi F., Sapino A., Aglietta M, & Sangiolo D. (2018). Cytokine Induced Killer cells are effective against sarcoma cancer stem cells spared by chemotherapy and target therapy.. Oncoimmunology, 7(11), e1465161.

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Examining NKG2D Ligand Expression in A549 Cells

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A549 cells were cultured in 12-well culture plates (3 × 10⁵/well) overnight. Then, cells were treated for 24 h with vehicle control (DMSO), gemcitabine or cisplatin. Expression of NKG2D ligands on A549 cells was analyzed by flow cytometry using anti-MICA/B (BD Biosciences), anti-ULBP1, anti-ULBP2/5/6 and anti-ULBP3 antibody. Anti-human ULBP1, ULBP2/5/6, and ULBP3 antibody were purchased from R&D Systems. Flow cytometry was carried out using an LSRIIsystem (BD Biosciences) and analyzed with FlowJo.

Zhang X., Wang D., Li Z., Jiao D., Jin L., Cong J., Zheng X, & Xu L. (2020). Low-Dose Gemcitabine Treatment Enhances Immunogenicity and Natural Killer Cell-Driven Tumor Immunity in Lung Cancer. Frontiers in Immunology, 11, 331.

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Immunophenotyping of Tumor Cells

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Cell lines were collected by trypsinization (Trypsin-EDTA, Thermo Fischer Scientific) and patient spheroids were digested using TrypLE (Thermo Fischer Scientific) and rested before the staining. Tumor cells were stained with viability cell dye (Zombie NIR, Biolegend) before the staining with anti-CD155 (Clone SKII.4), anti-CD112 (Clone TX31), anti-B7-H6 (Clone # 875001), anti-CD58 (Clone TS2/9), anti-MICA/B (Clone GD4), anti-HLA-ABC (BD Biosciences), anti-PD-L1 (Clone MIH2), anti-HLA class I Bw4 (Clone REA274), anti-HLA-E (Clone 3D12), and anti-EGFR (Clone AY13). Staining of patient derived tumor spheroids was subjected to sample availability.

Marin N.D., Becker-Hapak M., Song W.M., Alayo Q.A., Marsala L., Sonnek N., Berrien-Elliott M.M., Foster M., Foltz J.A., Tran J., Wong P., Cubitt C.C., Pence P., Hwang K., Zhou A.Y., Jacobs M.T., Schappe T., Russler-Germain D.A., Fields R.C., Ciorba M.A, & Fehniger T.A. (2024). Memory-like differentiation enhances NK cell responses against colorectal cancer. Oncoimmunology, 13(1), 2348254.

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