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200fr neo chemistry analyzer

Manufactured by Toshiba
Sourced in Japan

The 200FR NEO Chemistry Analyzer is a diagnostic instrument designed for automated biochemical analysis. It performs quantitative determination of various analytes in biological samples. The analyzer utilizes photometry and potentiometry techniques to provide accurate and reliable measurements.

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4 protocols using 200fr neo chemistry analyzer

1

Blood Biomarker Profiling Protocol

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After termination, approximately 3 mL of blood was held for a minimum of 90 min at room temperature and centrifuged (3000 rpm, 10 min, room temperature) to obtain serum. Glucose, blood urea nitrogen, total protein, albumin, creatinine, albumin/globulin ratio, triglyceride, phospholipid, total cholesterol, aminotransferase, aspartate aminotransferase, and creatine kinase were analyzed with a 200FR NEO Chemistry Analyzer (Toshiba Co., Japan).
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2

Comprehensive Urinalysis in Animal Study

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Animals were fasted overnight prior to urine collection. Urine samples were collected with a clean cage pan on wet ice (first void in the morning of each collection day), and the total fresh urine volume (VOL) was recorded. Urine samples were collected in a urine specimen container and kept cool with wet ice before analysis. Urinalysis was performed using a COBAS U 411 urine analyzer (Roche, Germany) and Combur 10 TM urine stick (Roche, Germany) to determine the following parameters: color, specific gravity (SG), pH, urine total protein (UTP), urine glucose (UGLU), urine creatinine (UCRE), urine albumin (UALB), and urine protein to creatinine ratio (P/C). Urine potassium (U-K), urine chlorine (U-Cl), and urine sodium (U-Na) were measured using a Toshiba 200FR NEO chemistry analyzer (Toshiba Co., Japan).
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3

Serum Separation from Blood Samples

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Blood samples (∼1.5 mL) were collected as described in section 2.2.6 and serum separated by mixing with anticoagulant. Tubes were maintained at room temperature for at least 90 min and then centrifuged (∼ 3000 rpm, 10 min, room temperature) to obtain serum. Serum parameters were analyzed using a Toshiba 200FR NEO Chemistry Analyzer (Toshiba Co., Japan).
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4

Primate Blood Analysis Protocol

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The parameters' abbreviations, units, and analysis methods are presented in Table 1. All animals were fasted overnight and blood samples were collected from the cephalic vein of non-anesthetized monkey for analysis of hematologic, coagulation, and serum chemistry parameters; if blood cannot be obtained from the cephalic vein, the saphenous or femoral vein was used. Blood samples were collected in tubes containing K2-EDTA for hematologic analysis. Hematological parameters were analyzed using an Advia 2120i hematology analyzer (Siemens, USA).
For serum chemistry analysis, blood samples were collected in tubes and placed at room temperature for at least 90 min prior to centrifugation (approximately 3000 rpm, 10 minutes, at room temperature). Serum chemistry parameters were measured using a Toshiba 200FR NEO chemistry analyzer (Toshiba Co., Japan).
In addition, blood samples were collected in tubes containing 3.2% sodium citrate and plasma samples were obtained after centrifugation (approximately 3000 rpm, 10 minutes, at room temperature) and analyzed for prothrombin (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) using an ACL 9000 coagulation analyzer (Instrumental Laboratory, Italy).
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