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2 protocols using monoclonal anti cea antibodies t86 66

1

Fabrication and Characterization of CEA-Targeted Fluorescent Nanoparticles

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The following analytical-grade chemicals were purchased from commercial sources and were used without further purification: L-glutamic acid (E), L-phenylalanine (F), indiocyaninegreen (ICG), human serum albumin (HSA), bovine plasma fibrinogen, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Matrigel, Triton-x-100 and monoclonal anti-CEA antibodies (T86-66) from Sigma (Rehovot, Israel); Poly(L-lactic acid) MW 2,000 Da from Polysciences (Warrington, PA, USA); N-hydroxysulfosuccinimide (Sulfo-NHS) and 2-morpholino ethanesulfonic acid (MES, pH 6) from Thermo Fisher Scientific (Rockford, IL, USA); Phosphate Buffered Saline (PBS), Minimum Essential Medium (MEM) eagle, McCoy’s 5A medium and Dulbecco’s modification of eagle’s medium (DMEM), Fetal Bovine Serum (FBS), glutamine, penicillin, streptomycin and mycoplasma detection kit from Biological Industries (Bet Haemek, Israel); cell cytotoxicity assay kit (LDH) from Roche (Switzerland); LS174t, SW480 and HT29 cell lines from American Type Culture Collection (ATCC); donkey anti-rabbit IgG from Jackson ImmunoResearch Laboratories (West Grove, PA, USA); water was purified by passing deionized water through an Elgastat Spectrum reverse osmosis system (Elga Ltd., High Wycombe, UK).
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2

Comprehensive Analytical-Grade Chemical Source Protocol

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The following analytical-grade chemicals were purchased from commercial sources and were used without further purification: L-glutamic acid (E), L-phenylalanine (F), ICG, human serum albumin (HSA), bovine plasma fibrinogen, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, Matrigel, PNA, Triton-x-100, fluorescein isothiocyanate-conjugated PNA (FITC-PNA), and monoclonal anti-CEA antibodies (T86-66) from Sigma (Rehovot, Israel); poly(L-lactic acid) MW 2,000 Da from Polysciences (Warrington, PA, USA); N-hydroxysulfosuccinimide (Sulfo-NHS) and 2-morpholino ethanesulfonic acid (MES, pH 6) from Thermo Fisher Scientific (Rockford, IL, USA); phosphate-buffered saline (PBS), minimum essential medium (MEM) eagle, McCoy’s 5A medium and Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), glutamine, penicillin, streptomycin, and mycoplasma detection kit from Biological Industries (Bet Haemek, Israel); a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5 carboxanilide salt (XTT) assay kit from Roche (Basel, Switzerland); and LS174t and SW480 cell lines from American Type Culture Collection. Water was purified by passing deionized water through an Elgastat Spectrum reverse osmosis system (Elga Ltd., High Wycombe, UK).
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