Nis elements system

Manufactured by Nikon

Sourced in Japan, United States

The NIS-Elements system is a comprehensive software platform designed to control and manage various Nikon microscopes and imaging devices. It provides a user-friendly interface for image acquisition, analysis, and processing. The NIS-Elements system offers a range of modules and tools to support various scientific and research applications, such as fluorescence imaging, time-lapse imaging, and image analysis.

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Lab products found in correlation

Dako antibody diluent, by Agilent Technologies (1 mentions) D1306, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Human mfish probes, by MetaSystems (1 mentions) Coolcubei ccd camera, by MetaSystems (1 mentions) Isis mfish software program, by MetaSystems (1 mentions) μ dishes, by Ibidi (1 mentions) Biostation im, by Nikon (1 mentions) Collagen a, by Harvard Bioscience (1 mentions) Anti α sma, by Merck Group (1 mentions) Digital microscope camera, by Nikon (1 mentions) 4 6 diamidino 2 phenylindole dapi, by ZSGB-BIO (1 mentions) Fstl1, by Santa Cruz Biotechnology (1 mentions) Axioimager z2 fluorescence microscope, by Zeiss (1 mentions) Mouse cot 1 dna, by Thermo Fisher Scientific (1 mentions) 49 6diamidino 2 phenylindole, by Merck Group (1 mentions)

Close spelling variants of this product

NIS-Elements (1183 citations) NIS-Elements BR 3.2 image analysis system (9 citations) NIS-Element imaging system (5 citations) NIS Elements AR system (3 citations) NIS elements BR 4.13.00 64-bit image analysis system (3 citations) NIS-Elements imaging system software (3 citations) NIS-Elements BR 3.0 system (2 citations) NIS-Elements AR 3.10 system (2 citations) NIS-Elements analysis system (2 citations) NIS-Elements Basic Research image acquisition system (2 citations)

9 protocols using nis elements system

Histomorphometric Analysis of Bone Regeneration

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A 5 cm segment of the bone including the defect site was harvested and fixed in 4% paraformaldehyde. The samples were then decalcified with hydrochloric acid, dehydrated in a series of ethanol solutions, and longitudinally embedded in paraffin. Next, samples were cut in the sagittal plane. The central longitudinal sections from each ulna were subsequently placed on the ground and cut to a thickness of 100 µm, after which they were stained with H&E and Massons's trichrome stain to evaluate new bone formation.
The stained sections from each group were observed under a light microscope and scanned using an attached digital camera and a NIS-Elements system (Nikon, Japan). For histomorphometric analysis, all groups were analyzed according to the following protocol. The entire implant area was viewed in six microscopic fields, and each field was captured using a digital camera and then subjected to histomorphometric analyses. The interface between the new bone and host bone in the histological picture was marked by measuring the distance from the screw hole in the radiographs. The newly formed bone area was estimated and converted into a percentage of the total implanted area using image processing and analysis software (ImageJ 1.49; National Institute of Health, USA).

Yoon D., Kang B.J., Kim Y., Lee S.H., Rhew D., Kim W.H, & Kweon O.K. (2015). Effect of serum-derived albumin scaffold and canine adipose tissue-derived mesenchymal stem cells on osteogenesis in canine segmental bone defect model. Journal of Veterinary Science, 16(4), 397-404.

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Immunostaining of Muscle Tissue Sections

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Briefly, frozen sections of TA muscles with a thickness of 5 μm were fixed in cold acetone for 5 min, followed by air-drying for 30 min. The tissues were then rinsed twice with Tris-buffered saline containing 0.05% tween-20 (TBS-T). Subsequently, the sections were treated with Dako Protein Block (serum-free, Cat#X0909) for 30 min at room temperature to block any nonspecific binding. After the blocking step, the sections were incubated overnight at 4 °C with primary antibodies diluted in Dako Antibody Diluent (Cat# S3022). The sections were then washed three times for 5 min each with TBS-T, followed by a 30-min incubation at room temperature with Alexa Fluor-conjugated secondary antibodies (either 488 anti-mouse or 546 anti-rabbit, both used at a 1:600 dilution, Invitrogen). After another round of washing with TBS-T three times, the sections were stained with DAPI (Thermo Fisher Scientific, D1306) in TBS-T for 5 min. Fluorescence images were captured using the NIS-Elements system (Nikon)^{46 (link)}. The antibodies used for both western blotting and immunostaining are listed in the Key Resources Table. To evaluate differences in the cross-sectional areas (CSA) of myofibers, 5 μm sections of TA muscles were stained with an anti-laminin antibody. The CSA of 300 myofibers per TA muscle was measured using Image J software (National Institutes of Health).

Lin H., Peng H., Sun Y., Si M., Wu J., Wang Y., Thomas S.S., Sun Z, & Hu Z. (2023). Reprogramming of cis-regulatory networks during skeletal muscle atrophy in male mice. Nature Communications, 14, 6581.

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Chromosome Enumeration and FISH Analysis of Microcell Hybrids

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Slides of microcell hybrids and ZFN-transfected clones were stained with quinacrine mustard and Hoechst 33258 to enumerate chromosomes. Images were captured using an AxioImagerZ2 fluorescence microscope (Carl Zeiss GmbH, Jena, Germany). FISH analyses were performed using fixed metaphases of microcell hybrids using digoxigenin-labelled (Roche, Basel, Switzerland) human Cot-1 DNA (Life Technologies) and biotin-labelled (Roche) pSTneo plasmid DNA essentially as described previously20 (link). Chromosomal DNA was counterstained with DAPI (Sigma-Aldrich). Images were captured using the NIS-Elements system (Nikon, Tokyo, Japan). mFISH analyses were performed in accordance with the manufacturer instructions (MetaSystems, Altlussheim, Germany). Human mFISH probes were purchased from MetaSystems GmbH. Metaphase images were captured digitally with a CoolCubeI CCD camera and the ISIS mFISH software program (MetaSystems).

Kazuki Y., Yakura Y., Abe S., Osaki M., Kajitani N., Kazuki K., Takehara S., Honma K., Suemori H., Yamazaki S., Sakuma T., Toki T., Shimizu R., Nakauchi H., Yamamoto T, & Oshimura M. (2014). Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies. Scientific Reports, 4, 6136.

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Wound Healing Assays in MDCK Cells

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For wound healing assays, MDCK cells were plated and grown to confluency on Ibidi μ-dishes (Ibidi, GmbH, Munich, Germany) coated with collagen A (Biochrom AG, Berlin, Germany). For quantification of migration speed, cells were incubated for 2 (unciliated) or 7–8 days (ciliated) ± 5μg/ml tetracycline and wounded using a micropipette. Cells were imaged using a Nikon Biostation IM (Nikon, Melville, USA), which includes a CO₂ incubation chamber equipped with heating unit and a motorized xy-table. Migration speed was calculated from 4 hours of cell migration between 1 and 8 hours after wounding. The semi automatic tracking properties of NIS ElementsSystem (Nikon, Melville, USA) software were used to quantify 5–6 fields of view per n. Migration assays with platings of approximately sparse 5000 cells were conducted with the same setup. Cells were treated with 10 ng/ml HGF (Hepatocyte Growth Factor, ImmunoTools, Friesoythe, Germany). Tracking of representative cells were made with Imaris software (version 7.1, Bitplane, Zurich, Switzerland).

Boehlke C., Janusch H., Hamann C., Powelske C., Mergen M., Herbst H., Kotsis F., Nitschke R, & Kuehn E.W. (2015). A Cilia Independent Role of Ift88/Polaris during Cell Migration. PLoS ONE, 10(10), e0140378.

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Measuring Mitochondrial Membrane Potential

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The mitochondrial membrane potential was measured using a mitochondrial membrane potential assay kit (JC-1, Beyotime Biotech, China) according to the manufacturer’s instructions. Cells were incubated with medium mixed with JC-1 working fluid in a 37 °C incubator for 20 min. The cells were washed with JC-1 buffer three times and replaced with fresh medium. Fluorescence was captured using confocal microscopy (A1R, Nikon, Japan). The ratio of red and green fluorescence was analyzed by an NIS-Elements system (Nikon, Japan). A high ratio indicated a high mitochondrial membrane potential.

Zhou Q., Xie M., Zhu J., Yi Q., Tan B., Li Y., Ye L., Zhang X., Zhang Y., Tian J, & Xu H. (2021). PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca2+ efflux. Stem Cell Research & Therapy, 12, 269.

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Quantifying Nascent Protein Synthesis in C2C12 Myotubes

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C2C12 myotubes were incubated in methionine free DMEM containing 50 μM HPG (Homo‐propargylglycine) for 30 min. Cells were fixed with 3.7% formaldehyde in PBS for 15 min, permeabilized with 0.5% Triton X‐100 in PBS for 20 min, washed twice with 3% bovine serum albumin in PBS and incubated in Click‐IT reaction cocktail (1X Click‐iT HPG reaction buffer, 2 mM CuSO₄, 3 μM Alexa Fluor azide, 1X Click‐iT HPG buffer additive) for 30 min at room temperature protected from light. Cells were washed with Click‐iT reaction rinse buffer then incubate with 1X HCS Nuclear‐Mask Blue Stain working solution for 30 min at room temperature. Images and fluorescence intensity measurements were taken using NIS‐Elements system (Nikon USA).

Yu R., Chen J., Xu J., Cao J., Wang Y., Thomas S.S, & Hu Z. (2016). Suppression of muscle wasting by the plant‐derived compound ursolic acid in a model of chronic kidney disease. Journal of Cachexia, Sarcopenia and Muscle, 8(2), 327-341.

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Quantifying Pulmonary Vascular Remodeling

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Serial sections in thickness of 4 μm were cut through the paraffin-embedded left lung lobe and stained with HE (Beijing Dingguo Changsheng Biotechnology, China). Anti-α-SMA (1:200, Sigma-Aldrich, USA) and FSTL1 (1:200, Santa Cruz, USA) were visualized by Alexa Fluor 594-labelled goat anti-mouse IgG and Fluorescein-conjugated rabbit anti-goat IgG (ZsBio, China), respectively, with nuclei mounted by 4′,6-diamidino-2-phenylindole (DAPI, ZsBio, China). Blood vessels were screened with a microscope digital camera (Nikon, Japan) and analyzed by NIS-Elements system (Nikon, Japan). Vascular remodelling was evaluated by MT% and numbers of completely muscularized arterioles52 (link)53 (link). Briefly, MT% was expressed as a percentage of ((external diameter - internal diameter)/external diameter). Arterioles exhibiting more than 75% of circumference positive for α-SMA were identified as completely muscularized arteries and their numbers were totaled in every 10 high-power (×400) fields. Transversely cut arterioles were included for measurement, with the exclusion of obliquely cut ones and pulmonary veins.

Zhang W., Wang W., Liu J., Li J., Wang J., Zhang Y., Zhang Z., Liu Y., Jin Y., Li J., Cao J., Wang C., Ning W, & Wang J. (2017). Follistatin-like 1 protects against hypoxia-induced pulmonary hypertension in mice. Scientific Reports, 7, 45820.

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Mitochondrial Membrane Potential Assay

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The mitochondrial membrane potential was measured using a mitochondrial membrane potential assay kit (JC-1, Beyotime Biotech, China) according to the manufacturer's instructions. Cells were incubated with medium mixed with JC-1 working uid in a 37℃ incubator for 20 min. Then, the cells were washed with JC-1 buffer three times and replaced with fresh medium. Finally, the uorescence was captured by confocal microscopy (A1R, Nikon, Japan). The ratio of red and green uorescence was analyzed by an NIS-Elements system (Nikon, Japan). A high ratio indicates a high mitochondrial membrane potential.

Zhou Q., Xie M., Zhu J., Yi Q., Tan B., Li Y., Ye L., Zhang X., Zhang Y., Tian J., & Xu H. (2020). PINK1 Contained in huMSCs-Exosomes Prevents Cardiomyocyte Mitochondrial Calcium Overload in Sepsis by Recovering Mitochondrial Ca2+ Efflux.

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FISH Analysis of Chromosome Spreads

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Trypsinized cells were incubated for 15 minutes in 75 mM KCl and fixed with methanol and acetic acid (3:1), and then slides were prepared using standard methods. FISH analyses were performed using fixed metaphase or interphase spreads of each cell hybrid with digoxigenin-labeled (Roche, Basel, Switzerland) DNA [human COT-1 DNA, mouse COT-1 DNA (Invitrogen), mouse minor satellite DNA (a gift from Dr. Vladimir Larionov, Head Investigator, Institution; National Cancer Institute, Developmental Therapeutics Branch, Bethesda, MD 20892, USA)] and biotin-labeled DNA [MDR1-BAC (RP11-784L5), mouse COT-1 DNA, CMV-BS, and PGK-Puro], essentially as described previously (Kazuki et al., 2013) . Chromosomal DNA was counterstained with 49,6diamidino-2-phenylindole (Sigma-Aldrich). Images were captured using the NIS-Elements system (Nikon, Tokyo, Japan) or an AxioImagerZ2 Fluorescence Microscope (Carl Zeiss, Oberkochen, Germany).

Yamasaki Y., Kobayashi K., Okuya F., Kajitani N., Kazuki K., Abe S., Takehara S., Ito S., Ogata S., Uemura T., Ohtsuki S., Minegishi G., Akita H., Chiba K., Oshimura M, & Kazuki Y. (2018). Characterization of P-Glycoprotein Humanized Mice Generated by Chromosome Engineering Technology: Its Utility for Prediction of Drug Distribution to the Brain in Humans. Drug metabolism and disposition: the biological fate of chemicals, 46(11).

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