Fam labeled probes and primers

Manufactured by Thermo Fisher Scientific

FAM-labeled probes and primers are fluorescent nucleic acid probes and primers that are labeled with the fluorescent dye FAM (6-carboxyfluorescein). FAM is a commonly used reporter dye in various molecular biology techniques, such as real-time PCR, for the detection and quantification of target DNA sequences.

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Lab products found in correlation

Taqman universal master mix, by Thermo Fisher Scientific (3 mentions) Abi prism 7700 sequence detection system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

FAM-labeled gene-specific probes and primers FAM-labeled primer/probes FAM-labeled probes FAM-labeled primers Primers and probes Primer probes

4 protocols using fam labeled probes and primers

Quantitative RT-PCR Analysis of mRNA

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Quantitative RT-PCR was carried out using commercially-available FAM-labeled probes and primers (Applied Biosystems). The mRNA amplification was calculated as previously (Kaur et al., 2007 (link)), and the data were plotted as the increase of fold change as compared with control samples. See supplemental information for a detailed description.

Saleiro D., Mehrotra S., Kroczynska B., Beauchamp E.M., Lisowski P., Majchrzak-Kita B., Bhagat T.D., Stein B.L., McMahon B., Altman J.K., Kosciuczuk E.M., Baker D.P., Jie C., Jafari N., Thompson C.B., Levine R.L., Fish E.N., Verma A.K, & Platanias L.C. (2015). Central Role of ULK1 in Type I Interferon Signaling. Cell reports, 11(4), 605-617.

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Quantitative Real-Time PCR Analysis

Cited 2 times

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Quantitative real-time PCR was performed in the ABI-7500 standard PCRs (Applied Biosystems) as described earlier (Ghosh et al., 2009 (link); Roy et al., 2013 (link)) using TaqMan Universal Master mix and FAM-labeled probes and primers (Applied Biosystems).

Roy A., Jana M., Kundu M., Corbett G.T., Rangaswamy S.B., Mishra R.K., Luan C.H., Gonzalez F.J, & Pahan K. (2015). HMG-CoA Reductase Inhibitors Bind to PPARα to Upregulate Neurotrophin Expression in the Brain and Improve Memory in Mice. Cell metabolism, 22(2), 253-265.

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Quantitative Real-Time PCR Analysis

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Real-time PCR analysis was performed in the ABI-Prism7700 sequence detection system (Applied Biosystems, Foster City, CA) as described earlier^{37 (link),39 (link)} using TaqMan Universal Master mix and FAM-labeled probes and primers (Applied Biosystems). Data were processed by the ABI Sequence Detection System 1.6 software and analyzed by ANOVA.

Roy A., Kundu M., Jana M., Mishra R.K., Yung Y., Luan C.H., Gonzalez F.J, & Pahan K. (2016). Identification and characterization of PPARα ligands in the hippocampus. Nature chemical biology, 12(12), 1075-1083.

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Quantitative Real-Time PCR Analysis

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