Paraformaldehyde (PFA) was purchased from Merck (Darmstadt, Germany), and 3,3'-diaminobenzidine 4HCl (DAB) was purchased from Dojin Chemical Co. (Kumamoto , Japan). Bovine serum albumin (BSA) (essentially fatty acid and globulin-free), Trizma base and Brij-35 were from Sigma Chemical Co. (St. Louis, MO, USA). Biotin-16-dUTP, digoxigenin-11-dUTP and terminal deoxynucleotidyl transferase (TdT) were from Roche Diagnostics (Mannheim, Germany). Dideoxy ATP (ddATP) and dideoxy TTP (ddTTP) were from Jena Bioscience (Jena, Germany). Hpa II, Msp I, Sau3A I and Mbo I were purchased from Takara Bio Inc. (Shiga, Japan). 4',6-Diamidino-2-phenylindole (DAPI) was from DAKO (Glostrup, Denmark). Permount was purchased from Fisher Scientific Inc. (NJ, USA). All other reagents used in this study were from Wako Pure Chemicals (Osaka, Japan) and were of analytical grade.
Dideoxy atp ddatp
Manufactured by Jena Biosciences
Sourced in Germany
Dideoxy ATP (ddATP) is a synthetic nucleotide analog that lacks a 3' hydroxyl group. It is commonly used in DNA sequencing techniques such as the Sanger method to terminate DNA chain elongation.
Automatically generated - may contain errors
Lab products found in correlation
Hpaii, by Takara Bio (2 mentions)
Dideoxy ttp ddttp, by Jena Biosciences (2 mentions)
Biotin 16 dutp, by Roche (2 mentions)
Digoxigenin 11 dutp, by Roche (2 mentions)
Brij 35, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Trizma base, by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole dapi, by Agilent Technologies (1 mentions)
Mbo 1, by Takara Bio (1 mentions)
Sau3ai, by Takara Bio (1 mentions)
Terminal deoxynucleotidyl transferase, by Roche (1 mentions)
Permount, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
2 protocols using dideoxy atp ddatp
1
Histochemical Staining with DAB and DAPI
2
Immunochemical Detection of DNA Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bovine serum albumin (BSA; essentially fatty acid and globulin-free), Trizma base, 2-mercaptoethanol, 3-aminopropyltriethoxysilane and Brij-35 were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany); 3,3′-Diaminobenzidine-4HCl (DAB) was purchased from Chemical Dojin Co., Ltd. (Kumamoto, Japan). Biotin-16-dUTP, digoxigenin-11-dUTP, Rhodamine anti-Dig and terminal deoxynucleotidyltransferase (TdT) were from Roche Diagnostics (Mannheim, Germany). Dideoxy ATP (ddATP) and dideoxy TTP (ddTTP) were from Jena Bioscience (Jena, Germany). HpaII and MspI were purchased from Takara Bio, Inc. (Otsu, Japan). DAPI was obtained from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Permount was from Thermo Fisher Scientific, Inc. All other reagents used in the present study were obtained from Wako Pure Chemicals Industries, Inc. (Osaka, Japan) and were of analytical grade.
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