The largest database of trusted experimental protocols

2 protocols using dideoxy atp ddatp

1

Histochemical Staining with DAB and DAPI

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paraformaldehyde (PFA) was purchased from Merck (Darmstadt, Germany), and 3,3'-diaminobenzidine 4HCl (DAB) was purchased from Dojin Chemical Co. (Kumamoto, Japan). Bovine serum albumin (BSA) (essentially fatty acid and globulin-free), Trizma base and Brij-35 were from Sigma Chemical Co. (St. Louis, MO, USA). Biotin-16-dUTP, digoxigenin-11-dUTP and terminal deoxynucleotidyl transferase (TdT) were from Roche Diagnostics (Mannheim, Germany). Dideoxy ATP (ddATP) and dideoxy TTP (ddTTP) were from Jena Bioscience (Jena, Germany). Hpa II, Msp I, Sau3A I and Mbo I were purchased from Takara Bio Inc. (Shiga, Japan). 4',6-Diamidino-2-phenylindole (DAPI) was from DAKO (Glostrup, Denmark). Permount was purchased from Fisher Scientific Inc. (NJ, USA). All other reagents used in this study were from Wako Pure Chemicals (Osaka, Japan) and were of analytical grade.
+ Open protocol
+ Expand
2

Immunochemical Detection of DNA Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bovine serum albumin (BSA; essentially fatty acid and globulin-free), Trizma base, 2-mercaptoethanol, 3-aminopropyltriethoxysilane and Brij-35 were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany); 3,3′-Diaminobenzidine-4HCl (DAB) was purchased from Chemical Dojin Co., Ltd. (Kumamoto, Japan). Biotin-16-dUTP, digoxigenin-11-dUTP, Rhodamine anti-Dig and terminal deoxynucleotidyltransferase (TdT) were from Roche Diagnostics (Mannheim, Germany). Dideoxy ATP (ddATP) and dideoxy TTP (ddTTP) were from Jena Bioscience (Jena, Germany). HpaII and MspI were purchased from Takara Bio, Inc. (Otsu, Japan). DAPI was obtained from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Permount was from Thermo Fisher Scientific, Inc. All other reagents used in the present study were obtained from Wako Pure Chemicals Industries, Inc. (Osaka, Japan) and were of analytical grade.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!