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3 protocols using nitric acid hno3

1

Selenium Bioanalytical Protocol

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Zinkuran SC was purchased from Arysta LifeScience Slovakia s.r.o. (Nové Zámky, Slovakia). Sodium selenate was obtained from Alfa Aesar (Karlsruhe, Germany). Nitric acid (HNO3, for mineral analysis) and hydrogen peroxide 30% (Suprapur) were purchased from LGC Standards (Molsheim, France) and Merck/VWR (Leuven, Belgium), respectively. Methanol was purchased from Biosolve (Valkenswaard, the Netherlands). Acetone was obtained from Merck (Darmstadt, Germany). Vanillin and (+)-catechin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid (HCl) was obtained from VWR (Leuven, Belgium).
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Selenium Speciation Analysis Protocol

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Zinkuran SC was purchased from Arysta LifeScience Slovakia s.r.o. (Nové Zámky, Slovakia). Sodium selenate was obtained from Alfa Aesar (Karlsruhe, Germany). Nitric acid (HNO3, for trace element analysis) and hydrogen peroxide 30% (Suprapur) were acquired from LGC Standards (Molsheim, France) and Merck/VWR (Leuven, Belgium), respectively. Sodium selenite (Na2SeO3), Sodium selenate (Na2SeO4), Se-methionine (SeMet), Se-cystine (SeCys2), and Se-methyl-selenocysteine (SeMetSeCys) were purchased from Sigma Aldrich (St. Louis, MO, USA). Protease XIV, citric acid, and methanol were from Sigma Aldrich. MilliQ (MQ) water from Water Systems Ltd. (Brussels, Belgium) was used throughout the experiment.
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3

Phytochemical Analysis of Zinkuran SC

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Zinkuran SC was acquired from Arysta LifeScience Slovakia s.r.o. (Nové Zámky, Slovakia). Sodium selenate was purchased from Alfa Aesar (Karlsruhe, Germany). Nitric acid (HNO3, for trace element analysis) was obtained from LGC Standards (Molsheim, France), and hydrogen peroxide (H2O2) 30% (Suprapur) was obtained from Merck/VWR (Leuven, Belgium). Methanol was purchased from Biosolve (Valkenswaard, the Netherlands). Acetone, sodium carbonate (Na2CO3), and sodium hydroxide (NaOH) were acquired from Merck (Darmstadt, Germany). Ascorbic acid (vitamin C), (+)-catechin, gallic acid, Folin–Ciocalteu's phenol reagent, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt, 2–2′-azobis (2-methylpropionamidine) dichloride (AAPH), aluminum chloride (AlCl3), and iron(II) sulfate were obtained from Sigma–Aldrich (St. Louis, MO, USA). Hydrogen chloride (HCl) and sodium nitrite (NaNO2) were obtained from VWR (Leuven, Belgium). Iron(III) chloride 6-hydrate was purchased from Merck (Darmstadt, Germany).
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