After the 4 hours POS pulse, cells were treated during the 3-day chase period with the following compounds: bafilomycin A1 (5–10 nM; Sigma-Aldrich, St. Louis, MO), Rab7 inhibitor (CID 1067700; 5–25 µM; Sigma-Aldrich), leupeptin (10 or 25 µM; Calbiochem, San Diego, CA) plus Pepstatin A (25 µM; Sigma-Aldrich) or a BACE 1 inhibitor (PF9283; 3–9 µM; Sigma-Aldrich). After the chase period, the cells were trypsinized for flow cytometry assays.
Rab7 inhibitor
Manufactured by Merck Group
The Rab7 inhibitor is a laboratory reagent that can be used to inhibit the activity of the Rab7 protein. Rab7 is a small GTPase involved in the regulation of late endosome and lysosome function. The Rab7 inhibitor can be used to study the role of Rab7 in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Pepstatin a, by Merck Group (2 mentions)
Bafilomycin a1, by Merck Group (2 mentions)
Pf9283, by Merck Group (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Glass bottom 96 well plate, by MatTek (1 mentions)
Eclipse ti, by Nikon (1 mentions)
Dynasore, by Merck Group (1 mentions)
Phenol red free dmem, by Thermo Fisher Scientific (1 mentions)
Volocity software, by PerkinElmer (1 mentions)
Cid1067700, by Merck Group (1 mentions)
3 protocols using rab7 inhibitor
1
Investigating Lysosomal Protein Degradation
2
Live Cell Imaging of C3aR-GFP Transfected Cells
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For imaging, medium was replaced to phenol red free DMEM (Thermo Fisher), with 20 mM HEPES (pH 7.3–7.4). Live cell imaging of C3aR-GFP transfected cell was carried out on glass bottom 96-well-plates (Mattek, Ashland, MA), with cells labeled with plasma membrane dye Wheat Germ Agglutinin (WGA) Deep Red (5 μg/mL; Thermo Fisher), mitochondrial maker MTDR (1 μM; Thermo Fisher) and nuclear marker Hoechst33342 (0.5 μg/mL; Thermo Fisher). Image acquisitions were performed using the UltraViewVoX spinning disk confocal microscope (Eclipse Ti, Nikon, Tokyo JPN), running Volocity software (Perkin Elmer, Wokingham UK) as published (21 (link)). Temperature and cellular pH were maintained using a table top incubator. Images were processed using ImageJ software (NIH). Some chemicals, dynasore, a dynamin inhibitor (200 μM; Sigma-Aldrich,), MnTBAP chloride, a superoxide dismutase mimetic (100 μM; Sigma-Aldrich) and CID1067700, an inhibitor of nucleotide binding by Ras-related GTPases (= Rab7 inhibitor; 100 nM; Sigma-Aldrich) were applied.
3
Lysosomal Inhibitors in Protein Trafficking
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After the 4h POS feeding, cells were treated during the 3 days chase period with the following compounds: Bafilomycin A1 (5-10 nM; Sigma-Aldrich), Rab7 inhibitor (CID 1067700; 5-25 µM; Sigma-Aldrich), Leupeptin (10 or 25 µM; Calbiochem) plus Pepstatin A (25 µM; Sigma-Aldrich) or a BACE 1 inhibitor (PF9283; 3-9 µM; Sigma-Aldrich). After the chase period, cells were trypsinized for flow cytometry assays.
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