Cultivated cells (1 × 106) were boiled in SDS-PAGE loading buffer, electrophoresed on 15% SDS-polyacrylamide gels, and transferred to a PVDF membrane using Xcell II blot module (Thermo Fisher, MA, United States). GLuc antibody (NEB, MA, United States) was used to detect GLuc protein. ATP-β antibody (Agrisera, Vännäs, Sweden) was used as a reference. HRP-conjugated goat anti-rabbit IgG (H + L) antibody (Life Technologies, CA, United States) was used as a secondary antibody. GLuc and ATP-β were visualized on an X-ray film by chemiluminescence using EPD Western Reagent (ELPIS-BIOTECH, Daejeon, South Korea).
Gluc antibody
Manufactured by New England Biolabs
Sourced in United States
The Gluc antibody is a protein-based detection reagent used in various analytical techniques. It is designed to specifically bind and detect the Gluc (Gaussia Luciferase) protein, which is commonly used as a reporter in biological assays. The core function of the Gluc antibody is to facilitate the identification and quantification of Gluc-tagged biomolecules or cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions)
Anti phosphorylated dna pkcs, by Abcam (2 mentions)
Bradford protein determination assay, by Bio-Rad (2 mentions)
Gapdh antibody, by Novus Biologicals (2 mentions)
Anti rad51, by Bioss Antibodies (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Ripa buffer, by Boston BioProducts (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Nitrocellulose membrane, by Bio-Rad (2 mentions)
Xcell 2 blot module, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg h l hrp conjugated antibody, by Thermo Fisher Scientific (1 mentions)
Epd western reagent, by Elpis Biotech (1 mentions)
3 protocols using gluc antibody
1
Western Blot Analysis of GLuc Protein
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in RIPA buffer (Boston Bio Products, Ashland, MA, USA) supplemented with a cocktail of protease inhibitors (5892791001, Roche, Basel, Germany) and phosphatase inhibitor (4906845001, Roche). Protein quantification was determined using the Bradford protein determination assay (Bio-Rad). A 30 μg sample of protein was loaded and resolved on a 10% NuPAGE BIS-TRIS gel (Life Technologies), then transferred to a nitrocellulose membrane (Bio-Rad) before incubation with primary antibodies. DNA-dependent protein kinase catalytic subunit (DNA-PKcs) antibody was obtained from Santa Cruz (sc-5282, Dallas, TX, USA) and anti-phosphorylated DNA-PKcs was purchased from Abcam (ab124918, Cambridge, MA, USA). Anti-RAD51 was purchased from BIOSS Antibodies (BSM-51402M, Woburn, MA, USA) and anti-β-actin was obtained from Cell Signaling Technologies (3700, Danvers, MA, USA). Gluc antibody was obtained from New England BioLabs (E8023). GAPDH antibody was obtained from Novus Biologicals (NB300–228, Centennial, Colorado, USA). Proteins were detected using SuperSignal West Pico Chemiluminescent Substrate (#34077, Thermo Fisher Scientific).
3
Western Blotting Analysis of DNA Repair Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sequencing results were analysed using the CRISPREsso web portal with average reading quality and single bp quality >30 according to the phred33 scale (26) .
Western blotting analysis. Cells were lysed in RIPA buffer (Boston Bio Products, Ashland, MA, USA) supplemented with a cocktail of protease inhibitors (5892791001, Roche, Basel, Germany) and phosphatase inhibitor (4906845001, Roche). Protein quantification was determined using the Bradford protein determination assay (Bio-Rad). A 30 µg sample of protein was loaded and resolved on a 10% NuPAGE BIS-TRIS gel (Life Technologies), then transferred to a nitrocellulose membrane (Bio-Rad) before incubation with primary antibodies. DNAdependent protein kinase catalytic subunit (DNA-PKcs) antibody was obtained from Santa Cruz (sc-5282, Dallas, TX, USA) and anti-phosphorylated DNA-PKcs was purchased from Abcam (ab124918, Cambridge, MA, USA). Anti-RAD51 was purchased from BIOSS Antibodies (BSM-51402M, Woburn, MA, USA) and anti-βactin was obtained from Cell Signaling Technologies (3700, Danvers, MA, USA).
Gluc antibody was obtained from New England BioLabs (E8023). GAPDH antibody was obtain from Novus Biologicals (NB300-228, Centennial, Colorado, USA).
Proteins were detected using SuperSignal West Pico Chemiluminescent Substrate (#34077, Thermo Fisher Scientific).
Western blotting analysis. Cells were lysed in RIPA buffer (Boston Bio Products, Ashland, MA, USA) supplemented with a cocktail of protease inhibitors (5892791001, Roche, Basel, Germany) and phosphatase inhibitor (4906845001, Roche). Protein quantification was determined using the Bradford protein determination assay (Bio-Rad). A 30 µg sample of protein was loaded and resolved on a 10% NuPAGE BIS-TRIS gel (Life Technologies), then transferred to a nitrocellulose membrane (Bio-Rad) before incubation with primary antibodies. DNAdependent protein kinase catalytic subunit (DNA-PKcs) antibody was obtained from Santa Cruz (sc-5282, Dallas, TX, USA) and anti-phosphorylated DNA-PKcs was purchased from Abcam (ab124918, Cambridge, MA, USA). Anti-RAD51 was purchased from BIOSS Antibodies (BSM-51402M, Woburn, MA, USA) and anti-βactin was obtained from Cell Signaling Technologies (3700, Danvers, MA, USA).
Gluc antibody was obtained from New England BioLabs (E8023). GAPDH antibody was obtain from Novus Biologicals (NB300-228, Centennial, Colorado, USA).
Proteins were detected using SuperSignal West Pico Chemiluminescent Substrate (#34077, Thermo Fisher Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!