Igma pe

Manufactured by BD

IgMa-PE is a fluorescently-labeled antibody designed for use in flow cytometry applications. It is a monoclonal antibody that specifically binds to the IgM surface antigen, which is expressed on certain types of B cells. The 'PE' in the name indicates that the antibody is conjugated to the fluorescent dye phycoerythrin.

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Lab products found in correlation

B220 apc, by Thermo Fisher Scientific (1 mentions) Cd3 percp cy5, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Fc block, by BD (1 mentions) Cd4 pe cy7, by BioLegend (1 mentions) Cd11c pe, by BioLegend (1 mentions) Cd8a percp, by BD (1 mentions) F4 80 alexa fluor 488, by BioLegend (1 mentions) Cd11b pe cy7, by BioLegend (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Facscanto, by BD (1 mentions)

Close spelling variants of this product

PE anti-IgMa

2 protocols using igma pe

Quantifying Peripheral B Cells After SpA Challenge

Cited 1 time

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To quantify the effect of SpA infusions on peripheral B cells, spleens were harvested 72 h post-SpA challenge and cell suspensions prepared, as described previously (43 (link), 46 (link), 56 (link)). Cells were maintained on ice throughout. For each mouse, 2 × 10⁶ cells were stained per panel in the presence of 20 μg/ml of Fc block (Pharmingen, Becton Dickinson) followed by staining with: B220-APC (eBioscience), CD3-PerCP Cy5.5 (BD), IgM^a-PE (BD) or IgM^b-PE (BD). For live/dead cell discrimination, we used fixable blue (Invitrogen) following the manufacturer's instructions. After staining, cells were washed and fixed in 4% paraformaldehyde. At least 5 × 10⁵ events per sample were collected on a LSR II flow cytometer (BD) running FACSDiva software. Data were analyzed with FlowJo (Treestar).

Ulloa-Morales A.J., Goodyear C.S, & Silverman G.J. (2018). Essential Domain-Dependent Roles Within Soluble IgG for in vivo Superantigen Properties of Staphylococcal Protein A: Resolving the B-Cell Superantigen Paradox. Frontiers in Immunology, 9, 2011.

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Multicolor Flow Cytometry Profiling of Pancreatic Immune Cells

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Single cell suspensions were prepared from resected pancreata using a gentleMACS Octo Dissociator with the Tumor Dissociation Kit as per the manufacturers’ instructions. Levels of the following cell surface markers were directly measured by flow cytometry on a BD FACSCanto (BD Biosciences) in two separate groups as noted and analyzed using FlowJo Software (Tree Star). Group 1: CD45 (APC; BioLegend), CD11b (PE/Cy7; BioLegend), Gr1 (PerCP; BioLegend), CD11c (PE; BioLegend), F4/80 (Alexa Fluor^® 488; BioLegend), Fixable Viability Dye (eFluor™ 780; eBioscience) Group 2: CD45 (APC; BioLegend), Th1.2 (Alexa Fluor^® 488; BioLegend), IgMa (PE; BD Pharmingen), CD4 (PE/Cy7; BioLegend), CD8a (PerCP; BD Pharmingen), Fixable Viability Dye (eFluor™ 780; eBioscience).

Nevler A., Muller A.J., Sutanto-Ward E., DuHadaway J.B., Nagatomo K., Londin E., O’Hayer K., Cozzitorto J.A., Lavu H., Yeo T.P., Curtis M., Villatoro T., Leiby B.E., Mandik-Nayak L., Winter J.M., Yeo C.J., Prendergast G.C, & Brody J.R. (2018). Host IDO2 gene status influences tumor progression and radiotherapy response in KRAS-driven sporadic pancreatic cancers. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(2), 724-734.

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