Trypsin powder

Eukaryotic expression vectors pMH3, pCApuro contained full-length IgG1 H& L chain were preserved in our lab. Restriction enzymes, T4 DNA Ligase and PCR mastermix were purchased from Thermo Scientific (Shanghai, China). Chinese hamster ovary (CHO-s) cell line was purchased from AmProtein (Hangzhou, Zhejiang, China). Human hepatoma cell line Huh-7, BEL-7402 and human hepatic cell line HL-7702 were preserved in our lab. Cell culture media and trypsin powder were purchased from Life Technologies (Basel, Switzerland). Human peripheral blood (PBL) was obtained from the Blood Center of Jiangsu Province (Nanjing, China). Six-week-old female BALB/c nude mice were purchased from Comparative Medicine Centre of Yangzhou University (Yangzhou, China). All animals were treated following the standards of Comparative Medicine Centre of Yangzhou University and all animal experiments were conducted under protocols approved by the Animal of the Ministry of Health of the People's Republic of China (Document No. 55, 2001).

Restriction enzymes were purchased from NEB (New England biolabs, Ipswich, MA, USA). T4 DNA Ligase was purchased from Thermo Scientific (Shanghai, China). Eukaryotic expression vectors pMH3-H, pCApuro-H, pMH3-L, pCApuro-L contained full-length IgG1 H & L chain were preserved in our lab27 (link). Chinese hamster ovary (CHO-s) cell line was purchased from AmProtein (Hangzhou, Zhejiang, China) and cultured in DMEM/F12 medium with 10% (v/v) fetal bovine serum (FBS). HUVEC cells purchased from ScienCell research laboratories (Carlsbad, CA, USA) were c\ultured in ECM medium supplement with 5% (v/v) FBS and 1% (v/v) ECGS (ScienCell, San Diego, CA, USA). Cell culture media and trypsin powder were purchased from Life Technologies (Basel, Switzerland).