Xc50 ccd camera

Cell density was measured using Coulter Z2 (Beckman, Indianapolis, IN, USA) equipped with 100 mm aperture or using the flow cytometer Guava EasyCyte 8HT (Merck KGaA, Darmstadt, Germany). Microscopy images were acquired with Olympus BX60 epifluorescence microscope coupled to XC50 CCD camera and equipped with plan fluorite objectives (Olympus, Center Valley, PA, USA) (air objectives 20×, NA = 0.5, 40×, NA = 0.75 and oil (type F, n = 1.518) objective 60×, NA = 1.25). Microscopy images were acquired with Olympus BX60 epifluorescence microscope coupled to the XC50 CCD camera. Scans of the entire preparations were performed with the Olympus IX83-motorized microscope equipped with the plan fluorite objective (20×, NA = 0.45). Images were analyzed in CellSens Standard (Olympus, Tokio, Japan) or Fiji. Results of flow cytometry were analyzed with Flowing Software 2.5.1. Statistical analysis was performed with GraphPad Prism 5 or STATISTICA version 7.1 (StatSoft, Inc., Tulsa, OK, USA). Uniform significance level was used through the entire text: * p < 0.05.

Microscopy images were captured with Olympus BX60 epifluorescent microscope coupled to XC50 CCD camera and analysed in Fiji [66 (link)]. Flow cytometry was performed using Guava EasyCyte 8HT (Merck) and analysed with Guava Soft 2.7 InCyte (Merck) or Flowing Software 2.5.1. Statistical analysis was performed with GraphPad Prism 5 and uniform significance levels were used through the entire manuscript: ^*p<0.05; ^**p<0.01; ^***p<0.001.

Brain slices were stained with 1% TTC (2,3,5-triphenyltetrazolium chloride) in phosphate-buffered solution (PBS) for 2–3 min at 38°C. Then slices were rinsed in PBS (for 1 min, 3 times). Microphotographs of TTC-stained slices were obtained using a SZX16 wide zoom stereo microscope equipped with a SDF PLAPO 1 × PF objective and SZX2-ILLT LED transmitted light illumination base (Olympus, Tokyo, Japan). Images were acquired at 0.7× -1× magnification using a XC50 CCD camera (Olympus, Tokyo, Japan) at 2,576 × 1,932 pixel resolution.