Anti granzyme b gb11

Manufactured by BD

Anti-granzyme B (GB11) is a laboratory reagent used for the detection and quantification of granzyme B, a serine protease enzyme involved in cell-mediated cytotoxicity. This antibody can be utilized in various immunoassay techniques, such as ELISA, to measure granzyme B levels in biological samples.

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Close spelling variants of this product

Anti-granzyme B-PE (clone GB11, Anti-granzyme B (clone GB11, PE-CF594-anti-granzyme B (clone GB11, Granzyme B (GB11) Anti-granzyme B Granzyme B

3 protocols using anti granzyme b gb11

Multiparametric Flow Cytometry for T Cell Phenotyping

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T cell phenotype was assessed by flow cytometry (LSRFortessa, BD) using fluorochrome-conjugated antibodies specific for CD3 (clone UCHT1; BD), CD4 (SK3; BD), CD8 (SK1; BD), CD45RO (UCHL1; BD), CX3CR1 (2A9-1; BioLegend), CD57 (HNK-1; BioLegend), CD27 (M-T271; BD), CD28 (CD28.2; BioLegend), CD69 (FN50; BD), and CCR7 (3D12; BD). Viable cells were gated using Live/Dead Aqua viability dye (Invitrogen). For induction and detection of intracellular cytokines, cells were cultured overnight with 20ng/ml recombinant human IL-15 (247-ILB; R&D Systems) or medium control (RPMI 1640 [Gibco], supplemented with 10% fetal bovine serum [Gemini Bio-Products], 1% L-glutamine [Gibco], and 1% penicillin/streptomycin [Gibco]), then treated with brefeldin A (GolgiPlug, BD) for 6h prior to harvest. After Live/Dead and surface staining, cells were fixed and permeabilized with Cytofix/Cytoperm (BD) for 20 min on ice and stained for 40 minutes on ice with anti-IFNγ (B27, BD) and anti-TNF (MAb11, BD). For intracellular accumulation of cytolytic molecules, cells were treated with IL-15-supplemented or control medium for 48 hours, then harvested, stained with Live/Dead and surface antibodies, treated with Cytofix/Cytoperm, stained with anti-granzyme B (GB11; BD) and anti-perforin (B-D48; BioLegend).

Panigrahi S., Chen B., Fang M., Potashnikova D., Komissarov A.A., Lebedeva A., Michaelson G.M., Wyrick J.M., Morris S.R., Sieg S.F., Paiardini M., Villinger F.J., Harth K., Kashyap V.S., Cameron M.J., Cameron C.M., Vasilieva E., Margolis L., Younes S.A., Funderburg N.T., Zidar D.A., Lederman M.M, & Freeman M.L. (2020). CX3CL1 and IL-15 Promote CD8 T cell chemoattraction in HIV and in atherosclerosis. PLoS Pathogens, 16(9), e1008885.

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Immunophenotyping of NK Cells and Leukemic Blasts

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Peripheral whole blood (3 ml) was obtained at diagnosis for immunophenotyping by flow cytometry of NK cells and leukemic blasts. The following antibodies were used to enumerate NK cells and their surface expression: anti-KIR2DL1 (143211), anti-NTB-A (292811), anti-ULBP1 (170818), anti-ULBP2 (165903), anti-ULBP3 (166510), anti-MICA (159227), and anti-MICB (236511) were obtained from R&D Systems; anti-KIR3DL1 (DX9), anti-KIR2DL2/3 (CH-L), anti-CD11a (HI111), anti-NKG2D (1D11), anti-TRAIL (RIK-2), anti-DNAM (DX11), anti-2B4 (2–69), and anti-granzyme B (GB11) from BD Bioscience; anti-NKG2A (Z199), anti-NKp30 (Z25), anti-NKp44 (Z231), anti-NKp46 (BAB281), and anti-FAS (CIB2) from Beckman Coulter; and anti-FASL (14C2) from AbD Serotec. Blasts obtained at diagnosis were evaluated by anti-NTB-A (292811) from R&D Systems; anti-ICAM (84H10), anti-FAS (CIB2) and anti-CD48 (J4-57) from Beckman Coulter; anti-Nectin (R2.525) from BD Bioscience, anti-TRAIL-R1 (DJR1) and anti-TRAIL-R2 (DJR2-4) from Biolegend; anti-PVR (TX21) from MBL International; anti-HLA-ABCEFG (W6/32) from Dako; and anti-PI-9 (7D8) from AbD Serotec.

Sullivan E.M., Jeha S., Kang G., Cheng C., Rooney B., Holladay M., Bari R., Schell S., Tuggle M., Pui C.H, & Leung W. (2014). NK Cell Genotype and Phenotype at Diagnosis of Acute Lymphoblastic Leukemia Correlate to Post-induction Residual Disease. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(23), 5986-5994.

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Multiparameter Analysis of NK Cells

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Seven fluorescently conjugated monoclonal antibodies specific for cell surface proteins were used to analyze NK cells. These comprised anti-CD19 (HIB19; eBioscience/Thermo Fisher Scientific), anti-CD-107a (eBioH4A3; eBioscience/Thermo Fisher Scientific), anti-KIR3DL1 (DX9; BioLegend), anti-NKG2A (REA110; Miltenyi Biotec), anti-LILRB1 (GHI/75; BioLegend), anti-panKIR2D (NKVFS1; Miltneyi), and anti-DNAM1 (11A8; BioLegend). Four fluorescently conjugated monoclonal antibodies were used to detect the following intracellular markers: anti-IFNγ (45.B3; BioLegend), anti-BrdU (51-23619L; BD Pharmingen), anti-granzyme B (QA16A02; BioLegend), and anti-granzyme B (GB11; BD Bioscience).

Pugh J., Nemat-Gorgani N., Djaoud Z., Guethlein L.A., Norman P.J, & Parham P. (2019). In vitro education of human natural killer cells by KIR3DL1. Life Science Alliance, 2(6), e201900434.

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