LAUD cells were plated in 6-well culture plates (5 × 103 cells/well). The medium was changed every 3 days. After 10 days, the cells were stained with a 0.005% Coomassie Brilliant Blue R-250 solution (Bio-Rad, USA), and the plates were imaged using an Epson Perfection V850 Pro Photo Scanner (USA).
Perfection v850 pro photo scanner
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The Epson Perfection V850 Pro Photo Scanner is a high-resolution flatbed scanner designed for professional photo and film scanning. It features a 6400 dpi optical resolution and can handle a variety of media including 35mm film, medium format film, and printed photographs.
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3 protocols using perfection v850 pro photo scanner
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Coomassie Staining of Cultured Cells
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Clonogenic Assay of Oxamate-Resistant Cells
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The clonogenic ability of MIAPaCA2 parental and sodium oxamate–resistant cells was measured under untreated and sodium oxamate–treated conditions. For these assays, 5 × 103 cells/well were seeded in 6-well culture plates. For drug treatment experiments, cells were treated with 10 mM of sodium oxamate. The medium containing sodium oxamate was changed every 3 days. After 10–14 days of treatment, surviving colonies were stained with a solution containing 40% methanol, 10% acetic acid, and 0.005% Coomassie Brilliant Blue R-250 (Sigma-Aldrich, USA), and the plates were imaged using an Epson Perfection V850 Pro Photo Scanner (USA).
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Quantification of Neuropathological Markers in Alzheimer's Disease
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The Harvard Brain and Tissue Resource Center (HBTRC) provided autopsied brain sections. Formalin‐fixed, paraffin‐embedded sections (5 μm) of the entorhinal cortex (ERc) and dorsolateral prefrontal cortex BA9 (DLPFC) from 35 subjects covering the entire span of AD neuropathology from Braak stage 0 to VI were used in this part of the study. The demographic characteristics of the patients are shown in Table 1 . The paraffin sections were rehydrated, and endogenous peroxidases were inactivated by treatment with 0.3% H2O2 in phosphate‐buffered saline (PBS) containing 0.03% Triton X‐100. The sections were incubated with primary antibodies at 4°C overnight and then with biotinylated secondary antibodies for 1 h. For visualization, sections were treated with avidin/biotin‐HRP complex (Vector) and then with 3,3′‐Diaminobenzidine substrate (Tokyo Chemical Industry Co., Ltd. Tokyo, Japan) with intensification by nickel ammonium sulfate. Photomicrographs were taken with an ECLIPSE E600 microscope (Nikon) equipped with a Mlchrome 5 Pro camera (Tucsen), and the brightness/contrast/threshold was adjusted with ImageJ 1.54f (NIH). Auto‐stitching tile scans were taken using the Mosaic 2 (Tucsen) live stitching function. Whole‐slide photos were taken with a well‐calibrated Perfection V850 Pro Photo Scanner (Epson).
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