70 μm cell strainer

Manufactured by BD

Sourced in United States, Germany, United Kingdom, Canada, Japan, Sweden

The 70-μm cell strainer is a laboratory equipment item designed to filter and separate cells. It features a mesh screen with 70-micron pores that allow the passage of single cells while retaining larger cellular aggregates or debris.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

70-μm-pore nylon cell strainers 70 and 40 μm cell strainers 70-μm and 40-μm cell strainers 100 and 70 μm cell strainers 70-μm pore-sized cell strainers 70-μm strainer Cell strainer

905 protocols using 70 μm cell strainer

Immune Cell Isolation from Murine Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was collected by cardiac puncture and mice were subsequently perfused with 20 mL cold PBS. Spleen, tumor, iliac lymph nodes, and femurs were harvested. Blood was incubated with red RBC Lysis buffer (BioLegend) for 4 minutes and washed with PBS containing 0.5% FBS. Spleens were mashed, filtered through a 70 μm cell strainer (BD Falcon), incubated with RBC Lysis buffer (BioLegend) for 4 minutes, and washed with PBS containing 0.5% FBS. Bone marrow was flushed out of the femur with PBS, filtered through a 70 μm cell strainer (BD Falcon), incubated with RBC Lysis buffer (BioLegend) for 30 seconds, and washed with PBS containing 0.5% FBS. Lymph node and tumor were pushed through a 70 μm cell strainer (BD Falcon) and washed with PBS containing 0.5% FBS.

Priem B., van Leent M.M., Teunissen A.J., Sofias A.M., Mourits V.P., Willemsen L., Klein E.D., Oosterwijk R.S., Meerwaldt A.E., Munitz J., Prévot G., Verschuur A.V., Nauta S.A., van Leeuwen E.M., Fisher E.L., de Jong K.A., Zhao Y., Toner Y.C., Soultanidis G., Calcagno C., Bomans P.H., Friedrich H., Sommerdijk N.A., Reiner T., Duivenvoorden R., Zupančič E., Di Martino J.S., Kluza E., Rashidian M., Ploegh H.L., Dijkhuizen R.M., Hak S., Pérez-Medina C., Bravo-Cordero J.J., de Winther M.P., Joosten L.A., van Elsas A., Fayad Z.A., Rialdi A., Torre D., Guccione E., Ochando J., Netea M.G., Griffioen A.W, & Mulder W.J. (2020). Trained immunity-promoting nanobiologic therapy suppresses tumor growth and potentiates checkpoint inhibition. Cell, 183(3), 786-801.e19.

+ Open protocol

+ Expand

Isolation of Human Prostate Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prostate tissues were digested in DMEM/F12/Collagenase/Hyaluronidase/FBS (Invitrogen, Carlsbad, CA) for 3 hours at 37 °C, followed by an additional 1 hour of digestion in 0.25% Trypsin-EDTA (Invitrogen, Carlsbad, CA) on ice. Subsequently, digested cells were suspended in Dispase (Invitrogen, Carlsbad, CA, 5 mg/mL) and DNase I (Roche Applied Science, Indianapolis, IN, 1 mg/mL), and pipetted vigorously to dissociate cell clumps. Dissociated cells were then passed through 70 μm cell strainers (BD Biosciences, San Jose, CA) to get single cells.
Human prostate tissues were chopped into 2–3 mm-long pieces and incubated in 5 mg/ml collagenase type II/ advanced DMEM/F12 (1 ml per 50 mg of prostate tissue) with 10 μM of Y-27632 (STEMCELL technologies) for 5–12 hours. Tissues were pelleted, resuspended, and incubated in chilled 0.25% Trypsin-EDTA for 5 min. Thereafter, human prostate tissues were pelleted, resuspended in Dispase (Invitrogen, Carlsbad, CA, 5 mg/mL) and DNase I (Roche Applied Science, Indianapolis, IN, 1 mg/mL), and pipetted vigorously to dissociate cell clumps. Dissociated cells were then passed through 70 μm cell strainers (BD Biosciences, San Jose, CA) to obtain single cells.

Zhou Z., Jia D., Kwon O., Li S., Sun H., Roudier M.P., Lin D.W., True L., Morrissey C., Creighton C.J., Lee J.K, & Xin L. (2023). Androgen-regulated stromal complement component 7 (C7) suppresses prostate cancer growth. Oncogene, 42(32), 2428-2438.

+ Open protocol

+ Expand

Tissue Digestion and Single-Cell Suspension Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ears were dissected into small pieces and digested in 500 μl RPMI containing 10% FCS and 1 mg/ml Collagenase P (Roche Diagnostic) for an initial 1 hr at 37 °C. The digested tissues were briefly spin at 2,000 rpm for 5 min and the 200 μl clear supernatant was collected and stored at -20 °C for LEGENDplex analysis. The remaining tissue pellets were resuspended thoroughly with another 1 ml of RPMI + Collagenase P with another 1 hr incubation at 37 °C, and 100 µg/ml DNase I (Roche Diagnostic) was added for the final 30 min. The digested samples were passed through 70-μm cell strainers (BD Biosciences) and the digestion was stopped with 500 µl cold 10 mM EDTA in PBS, and a single cell suspension was obtained in FACS cell staining buffer (BioLegend). Auricular lymph nodes were harvested and meshed through 70-μm cell strainers (BD Biosciences) to obtain single cell suspensions in FACS cell staining buffer (BioLegend).

Chen Y.L., Ng J.S., Babu R.O., Woo J., Nahler J., Hardman C.S., Kurupati P., Nussbaum L., Gao F., Dong T., Ladell K., Price D.A., Duncan D.A., Johnson D., Gileadi U., Koohy H, & Ogg G.S. (2023). Group A streptococcus induces CD1a-autoreactive T cells and promotes psoriatic inflammation. Science immunology, 8(84), eadd9232.

+ Open protocol

+ Expand

Isolation of CNS and Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were perfused with 30 mL PBS via the heart before the isolation of spinal cords and brains. The tissues were filtered through 70 μm cell strainers (BD, USA) to yield single-cell suspensions in IMDM containing 10% FBS (invitrogen, USA). CNS mononuclear cells (MNCs) were purified using a Percoll (GE Healthcare, USA) gradient (70/37%).
Spleens and lymph nodes extracted from C57BL/6 mice were teased through sterilized 70 μm cell strainers (BD, USA) to obtain single-cell suspensions in IMDM containing 10% FBS (invitrogen, USA) medium. Red blood cells were lysed with RBC lysis buffer (eBioscience, USA). CD4⁺ T cells were purified by using anti-CD4 magnetic beads (Miltenyi biotech, Germany).

Guo W., Wang C., Wang X., Luo C., Yu D., Wang Y., Chen Y., Lei W., Gao X, & Yao W. (2015). A novel human truncated IL12rβ1-Fc fusion protein ameliorates experimental autoimmune encephalomyelitis via specific binding of p40 to inhibit Th1 and Th17 cell differentiation. Oncotarget, 6(30), 28539-28555.

+ Open protocol

+ Expand

Isolation of Spleen and Tumor Infiltrating Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleen cells were isolated by scissor dissociation, collected in RPMI 1640–10% FBS, and filtered using a 70 μm cell strainer (Becton Dickinson (BD), San Diego, CA, USA). Erythrocytes in the splenic preparations were removed by hypotonic lysis with NH4Cl–KHCO3–EDTA solution. Tumor infiltrating leucocytes from C51 colon carcinoma were obtained by cutting the tumor mass into small pieces followed by incubation (1 g/10 mL) with a mixture of enzymes dissolved in serum free RPMI: collagenase type I (0.5 mg/mL), collagenase type IV (0.5 mg/mL), and hyaluronidase (0.25 mg/mL), all from Sigma Aldrich, and DNase I (0.1 mg/mL) from Roche (Mannheim, Germany) for 30 min at 37 °C. Undigested material was removed by filtration using a 70 μm cell strainer (BD) and the single cell suspensions were recovered and washed by centrifugation in RPMI 1640–10% FBS to remove residual enzymes.

De Vito A., Orecchia P., Balza E., Reverberi D., Scaldaferri D., Taramelli R., Noonan D.M., Acquati F, & Mortara L. (2020). Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection. Cancers, 12(3), 717.

+ Open protocol

+ Expand

Immune Cell Dynamics After Spinal Cord Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols

To study the dynamics of immune cells after SCI, spinal cords from sham (laminectomy only) and injured LV-GFP or LV-ChABC treated rats were harvested at day 1, 3, 7, 14 and 28 days after lesion. Animals were deeply anaesthetised with sodium pentobarbital (Euthatal^®, 80 mg/kg, administered intraperitoneally) and transcardially perfused with ice-cold 1X phosphate buffered saline (PBS) + 2% EDTA. Immediately after perfusion, 8 mm of the injured spinal cord centred around the lesion epicentre was dissected and placed into ice-cold PBS. Tissue was mechanically dissociated and then passed through a 70 μm cell strainer (BD Falcon, Germany), and centrifuged at 300 × g at 4 °C. The pellet was incubated with Myelin Removal Beads II (Miltenyi Biotec, Germany) and passed through LS columns (Miltenyi Biotech) to elute cells. For primary cell cultures, cells were detached by incubating with Enzyme Free cell dissociation buffer (Milipore). Remaining adherent cells were gently scraped and passed through a 70 μm cell strainer (BD Falcon, Germany). After centrifugation, cells were resuspended in DPBS (Gibco).

Francos-Quijorna I., Sánchez-Petidier M., Burnside E.R., Badea S.R., Torres-Espin A., Marshall L., de Winter F., Verhaagen J., Moreno-Manzano V, & Bradbury E.J. (2022). Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury. Nature Communications, 13, 2933.

+ Open protocol

+ Expand

Isolation of Tumor-Infiltrating Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions were prepared from dissected tumors and spleens. Spleens were mechanically disrupted using a plunger end of a 5 mL syringe and resuspended in 1% FBS/PBS after passing through a 70-μm cell strainer (Falcon). Red blood cells were depleted from total splenocytes using 1x RBC Lysis Solution (eBioscience, 00–4333-57). For isolation of tumor-infiltrating lymphocytes, tumor tissue was minced into 1 to 2 mm pieces and digested with collagenase IV (1.25 mg/mL; #LS004188, Worthington), 0.1% trypsin inhibitor from soybean (# T9128, Sigma), hyaluronidase (1 mg/mL; # LS 002592, Worthington), and DNase I (100 mg/mL; # LS002007, Worthington) in complete DMEM for 30 minutes at 37°C. Cell suspensions were passed through a 70-μm cell strainer (Falcon) and resuspended in RPMI media (Gibco). Lymphocytes were isolated from processed tumor tissues by OptiPrep (Sigma) density gradient centrifugation. For experiments requiring ex vivo stimulation and intracellular cytokine staining, regulatory B cells (CD19⁺CD1d^HiCD5⁺CD21^Hi) and conventional B cells (CD19⁺CD1d^LoCD5^-CD21^Lo) were sorted from single cell splenic suspensions using the BD FACS Aria III cell sorter according to manufacturer’s instructions and UNC flow cytometry core guidelines.

Michaud D., Mirlekar B., Bischoff S., Cowley D.O., Vignali D.A, & Pylayeva-Gupta Y. (2019). Pancreatic cancer-associated inflammation drives dynamic regulation of p35 and Ebi3.. Cytokine, 125, 154817.

+ Open protocol

+ Expand

Isolation of Lymph Node and Skin Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inguinal lymph nodes were mechanically disaggregated and digested in 1 mL non-supplemented RPMI 1640 medium (ThermoFisher Scientific, ref 61870-036) containing 5 mg/mL of collagenase type IV (Gibco, ref 17104019) and 5 µg/mL of DNAse I (AppliChem, ref A3778,0010) for 30 min at 37 °C. Single-cell suspension was obtained using a 70 μm cell strainer (BD Falcon, ref 352350). For skin preparations, vaccinated skin was excised, cut in small fragments and digested in 1 mL non-supplemented RPMI 1640 medium (ThermoFisher Scientific, ref 61870-036) containing 5 mg/mL of collagenase type IV (Gibco, ref 17104019) and 5 µg/mL of DNAse I (AppliChem, ref A3778,0010) for 30 min at 37 °C, skin pieces were then disaggregated mechanically using microscope slides with ground edges (Sail Brand, ref 7105) and single-cell suspension was obtained using a 70 μm cell strainer (BD Falcon, ref 352350) followed by a second digestion with 1 mL of supplemented of RPMI 1640 medium containing 5 µg/mL of DNAse I (AppliChem, ref A3778,0010) during 5 min on ice.

Menares E., Gálvez-Cancino F., Cáceres-Morgado P., Ghorani E., López E., Díaz X., Saavedra-Almarza J., Figueroa D.A., Roa E., Quezada S.A, & Lladser A. (2019). Tissue-resident memory CD8+ T cells amplify anti-tumor immunity by triggering antigen spreading through dendritic cells. Nature Communications, 10, 4401.

+ Open protocol

+ Expand

Isolation of Mammary Tumor Cells and Immune Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mammary tumor epithelial cells (MECs) were isolated from MMTV-Wnt1 or MMTV-Wnt1/dnIGF-1R mice similarly to our prior study [1 (link)]. Whole tumors were excised and dissociated with the gentleMACs tissue dissociator (130–093-235, protocol m_TDK2) and mouse specific tumor dissociation kit (Miltenyi, 130–096-730). Organoids that retain basement membrane attachments were trypsinized (0.05% Trypsin-EDTA, Gibco) and filtered with a 40-μm cell strainer (BD Biosciences) to isolate a single cell suspension of dissociated tumor MECs. Isolated tumor MECs were counted using a hemocytometer prior to flow cytometry or sorting.
Mammary tumor immune cells were isolated from tumors as described previously [23 ]. Whole tumors were excised, minced, and digested with Collagenase-I (10 U/ml), Collagenase-IV (400 U/ml; Worthington), and DNase-1 (30 mg/ml; Sigma Aldrich) for 25 min at 37 °C. Cells from digested tumors were filtered with a 70-μm cell strainer (BD Biosciences) and pelleted. Red blood cells were lysed with an erythrocyte lysis buffer (150 mM Ammonium chloride, 1 mM Potassium bicarbonate, 130 μM EDTA, pH 7.2) for 2 min, filtered with a 70-μm cell strainer (BD Biosciences) and pelleted. Isolated immune cells were counted using a hemocytometer prior to flow cytometry or magnetic bead sorting.

Obr A.E., Kumar S., Chang Y.J., Bulatowicz J.J., Barnes B.J., Birge R.B., Lazzarino D.A., Gallagher E., LeRoith D, & Wood T.L. (2018). Insulin-like growth factor receptor signaling in breast tumor epithelium protects cells from endoplasmic reticulum stress and regulates the tumor microenvironment. Breast Cancer Research : BCR, 20, 138.

+ Open protocol

+ Expand

Hepatic Leukocyte Isolation for Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspension of hepatic leukocytes were prepared using a conventional method according to a previous report with some modifications [17 (link)]. Briefly, infected or control mice were perfused with phosphate-buffered saline (PBS) via the portal vein. The excised liver was minced, then pressed through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA). The total hepatic cells were then resuspended in 40% Percoll solution (GE Healthcare, Chicago, IL, USA), and centrifuged for 15 min at 750× g. Then, the leukocytes were resuspended in erythrocyte-lysing buffer. Lastly, the cells were washed and resuspended in RPMI 1640 medium. For the spleen, all cells were washed and resuspended in RPMI 1640 medium immediately after they had been pressed through a 70-μm cell strainer (BD Biosciences).

Sun L., Gong W., Shen Y., Liang L., Zhang X., Li T., Chen T.T., Hu Y, & Cao J. (2020). IL-17A-producing γδ T cells promote liver pathology in acute murine schistosomiasis. Parasites & Vectors, 13, 334.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!