The largest database of trusted experimental protocols

28 protocols using delta 320 ph meter

1

Pork Muscle Quality Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Forty-five minutes and 24 h after slaughter, three 12 x 12 x 5 cm samples of the longissimus muscle at the last rib level were obtained to determine the color (Opto-star color analyzer, Matthaus, Germany), marbling, and pH of skeletal muscle (DELTA 320 pH meter; Mettler Toledo, Greifensee, Switzerland). Marbling scores were determined by the marbling standards of the National Pork Producers Council, and the average value of 10 judges' marks was recorded. Five pH measurements were performed for each sample (5 small incisions were made in the meat and the pH meter probe was placed into the incisions to test muscle pH), and the average value was recorded as the sample pH (Norman et al., 2004) .
+ Open protocol
+ Expand
2

Analysis of Brine Chemical Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pH of these brine samples value was measured by a Delta 320 pH meter (Mettler-Toledo, Zurich, Switzerland). Additionally, chemical composition of them was performed by a commercial analytical laboratory based on standardized methods (Beijing Zhongkebaice Technology Service Co., Ltd., Beijing, China). Nitrate ion (NO3) concentration was measured by ion-chromatography on an ICS-1500 (Dionex, Sunnyvale, CA, United States). The content of metallic elements such as magnesium (Mg), iron (Fe), and potassium (K) were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) using an OPTIMA 5300 DV spectrometer (Perkin Elmer, Norwalk, CT, United States).
+ Open protocol
+ Expand
3

Soil Chemical and Physical Properties

Check if the same lab product or an alternative is used in the 5 most similar protocols
Soil pH was determined in a soil and distilled water (1:2.5, W/V) mixture using a Delta 320 pH meter (Mettler-Toledo Instruments, Shanghai, China). Soil moisture was gravimetrically measured by oven drying at 105°C for 24 h. Soil organic matter was determined with the potassium dichromate external heating method (Guo 2009) (link). Soil total carbon (TC) and total nitrogen (TN) were measured with an elemental analyzer (Vario MAX C/N, Hanau, Germany) (Parkinson and Allen 1975) (link). Determination of alkaline hydrolyzable N, calcium (Ca 2+ ), and magnesium (Mg 2+ ) was based on the Chinese national standard method (Nu 1999).
+ Open protocol
+ Expand
4

Soil Physicochemical Properties Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Soil pH was measured using a soil to water ratio of 1:2.5 using a Delta 320 pH meter (Mettler Toledo Instruments, Shanghai, China). The SOC content was determined with a macro elemental analyzer (Vario MAX C/N; Elementar Analysensysteme, Hanau, Germany). The total N content was determined by Kjeldahl digestion, and the total P concentration was measured using the ammonium molybdate method following H2SO4-H2O2-HF digestion. The soil available N (the sum of NH4-N and NO3-N) was measured with an FIA Star 5000 flow-injection autoanalyzer (Foss Tecator, Höganäs, Sweden).
+ Open protocol
+ Expand
5

Fully-automated DLLME system for rice arsenic analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The fully-automated DLLME system (Fig. 1) comprised a rotary 8-port multi-position valve (VICI, Schenkon, Switzerland) coupled to an automatic burette with a 10 mL syringe (Hamilton, Bonaduz, Switzerland) and a three-way solenoid valve (Jiashan Ristron electronic technology Co., Ltd., Zhejiang, China) in the head section. Multiwave 3000 XF100 (Anton Paar, Graz, Austria) microwave digestion system was used for rice digestion. A research Delta 320 pH-meter (Mettler-Toledo, Zurich, Switzerland) were employed for measurement of pH values. The magnetic stirring system was composed by a lab made device and a small magnetic stirrer bar placed inside the syringe, as detailed in previous study.30 (link) A Perkin-Elmer Model AAnalyst 800 atomic absorption spectrometer (Perkin-Elmer, USA) with Zeeman effect background correction was utilized as the detection system. An arsenic hollow cathode lamp operated at 6 mA was utilized as a radiation source. The spectral bandwidth was set at 0.7 nm and the wavelength was set at 193.7 nm resonance line. The detailed temperature program used for graphite atomizer is listed in Table 1. All tubing of the fully-automated DLLME systems was made from polytetrafluoroethylene (PTFE) tubing with 0.79 mm inner diameter.
+ Open protocol
+ Expand
6

Cecal pH and VFA Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The contents of the cecum were immediately taken out, and the pH value was quickly determined using a DELTA 320 pH meter (Mettler-Toledo, Shanghai, China). Furthermore, 2 mL of distilled water was added to 2 mL of cecal contents and centrifuged at 10,000 rpm for 10 min. Then, 1 mL of supernatant was taken, 0.2 mL of 20% metaphosphoric acid solution containing 60 mM crotonic acid was added, and 0.4 μL of supernatant was taken for injection analysis. The VFA was measured using a GC-9A Gas Chromatograph (Shimadzu, Kyoto, Japan), with nitrogen as the carrier gas and a flow rate of 30 mL/min. The standard solutions were 3.353 mg/mL acetic acid, 1.189 mg/mL propionic acid, and 0.793 mg/mL butyric acid. In addition, after centrifuging 4 mL of cecal content diluent for 10 min, 50 μL of the supernatant was taken in a 10-mL test tube. Subsequently, 3 mL each of phenol and sodium hypochlorite was added to the test tube. The test tube was cooled in a 60°C water bath for 10 min and then immediately with cold water. Then, the optical density (OD) at 546 nm was determined using a UV756 spectrophotometer (UNICO, CA, United States) to calculate the NH3-N concentration in the cecum. The ammonia standard stock solution contained 32 mg/100 mL NH3-N.
+ Open protocol
+ Expand
7

Comprehensive Chemical Characterization of Substrate

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pH value was determined with a Delta 320 pH meter (Mettler-Toledo International Inc., Switzerland) at substrate: water = 1:5. The content of organic matter was determined using the potassium dichromate volumetric method. Using the Kjeldahl determination, the content of total nitrogen was investigated in substrate after heating digestion by concentrated sulfuric acid. The contents of total phosphorus, total potassium, and heavy metals were determined by ICP-AES (6,300, Thermo Fisher Scientific Inc., USA) after microwave digestion.
+ Open protocol
+ Expand
8

Comprehensive Material Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All materials were characterized by these instruments. FTIR spectra were recorded by JASCO FT-IR-4100 (Japan) spectrometer in the range of 4000–400 cm−1. The XRD was obtained by Brukeraxs D8, Germany. A copper Ka radiation target with a wavelength of 1.54 Å was employed. The morphology of the composite was examined using a scanning electron microscope (SEM: JEOL and JSM-6510LV) and a transmission electron microscope (TEM: JEM-2100 JEOL (Japan). The energy dispersive X-ray spectroscopy (EDX) was analyzed by an IT100LA operating at an accelerating voltage of 20.00 keV that was attached to the SEM device. The XPS analysis was made by XPS (PHI Quantera SXM; ULVAC-PHI, Inc., Osaka, Japan).
The specific surface area was measured using the BET (Belsorb III equipment, Japan) with nitrogen as the adsorbate. The pH data were obtained with a Mettler Delta 320 pH meter. A UV/Vis double beam spectrophotometer (PG T80+, U.K.) was employed to monitor the progress of MB adsorption with time.
+ Open protocol
+ Expand
9

Comprehensive Material Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All materials were characterized by these instruments. FTIR spectra were recorded by JASCO FT-IR-4100 (Japan) spectrometer in the range of 4000–400 cm−1. The XRD was obtained by Brukeraxs D8, Germany. A copper Ka radiation target with a wavelength of 1.54 Å was employed. The morphology of the composite was examined using a scanning electron microscope (SEM: JEOL and JSM-6510LV) and a transmission electron microscope (TEM: JEM-2100 JEOL (Japan). The energy dispersive X-ray spectroscopy (EDX) was analyzed by an IT100LA operating at an accelerating voltage of 20.00 keV that was attached to the SEM device. The XPS analysis was made by XPS (PHI Quantera SXM; ULVAC-PHI, Inc., Osaka, Japan).
The specific surface area was measured using the BET (Belsorb III equipment, Japan) with nitrogen as the adsorbate. The pH data were obtained with a Mettler Delta 320 pH meter. A UV/Vis double beam spectrophotometer (PG T80+, U.K.) was employed to monitor the progress of MB adsorption with time.
+ Open protocol
+ Expand
10

Soil Properties Monitoring in Dongmen Forest

Check if the same lab product or an alternative is used in the 5 most similar protocols
Air temperature and precipitation in the Dongmen Forest Farm (Fig 2) were monitored using a rainfall recorder L99-YLWS (Shanghai Fotel LTD., Shanghai, China).
In August 2013 and 2014, 0–20 cm surface soils near the gas sampling points were sampled. Soil pH, dissolved organic carbon (DOC), ammonia N (NH4+-N), and nitrate N (NO3--N) were measured. To measure soil pH, 10 g of air-dried soil were combined with 25 ml of deionized water. The slurry was swirled gently by hand and allowed to settle for 30 min. A Delta 320 pH meter [Mettler-Toledo Instruments (Shanghai) Co., Ltd., Shanghai, China] was used to measure pH of the supernatant. To measure DOC, the soil was extracted with a 0.5 M K2SO4 solution (soil:solution ratio of 1:5) by shaking for 30 min and the extracts were filtered through filter paper (45 μm). The filtrates were analyzed using a TOC analyzer (Liqui TOC, Elementar, Hanau, Germany). To measure NH4+-N and NO3--N, the soil was extracted with a 2 M KCl solution (soil:solution ratio of 1:5) by shaking for 30 min and the extracts were filtered through filter paper (45 μm). The filtrates were analyzed using a continuous flow analyzer (San++, SKALAR, Breda, Netherlands).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!