Sc30 digital camera

Manufactured by Olympus

Sourced in Denmark, Japan, United States

The SC30 is a digital camera designed for laboratory use. It features a 3-megapixel image sensor and supports various image formats, including JPEG and TIFF. The camera offers basic imaging capabilities for visual documentation and analysis purposes.

Automatically generated - may contain errors

Lab products found in correlation

Sz61 stereomicroscope, by Olympus (2 mentions) Bx46 microscope, by Olympus (2 mentions) Ckx41 inverted microscope, by Olympus (2 mentions) Tcs sp5, by Leica (1 mentions) Cd11b fitc m1 70, by BD (1 mentions) Ly6g pe 1a8, by BioLegend (1 mentions) Axioplan microscope, by Olympus (1 mentions) Gl7 fitc, by BD (1 mentions) Cd138 pe 281 2, by BD (1 mentions) B220 apc ra3 6b2, by BD (1 mentions) Bx43 microscope, by Olympus (1 mentions) Hm 340e electronic rotary microtome, by Thermo Fisher Scientific (1 mentions) Phenol red, by Thermo Fisher Scientific (1 mentions) Phase contrast microscope, by Olympus (1 mentions) Dmem high glucose, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Szx16 stereomicroscope, by Olympus (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions)

Close spelling variants of this product

Digital camera (379 citations) SC30 camera (46 citations)

10 protocols using sc30 digital camera

Floral Visitors and Pollen Collection Behavior

Check if the same lab product or an alternative is used in the 5 most similar protocols

Focal observations were made on days with clear skies, with the help of scaffolding installed next to the plants. Images were captured with a Nikon DSLR D7200 Digital Camera. Pinheiro et al. (2018) studied the floral visitors of both species (however, S. flaemingii was referred to as ‘Swartzia oblata’), while our observations were focused on the pollen collection behaviour and interaction of the animals with the floral parts. Visitors were grouped according to their pollen gathering behaviour following the terminology proposed by Portman et al. (2019) . These behaviours include: scraping with the extremities, buzzing, rubbing with the body and/or scopae, rubbing with the face, tapping and rasping. Several bees were captured for identification by a specialist (see Acknowledgements).
The opening dynamics of the anthers were complemented with an Olympus SZ61 stereomicroscope with an Olympus SC30 digital camera.

Basso-Alves J.P., da Silva R.F., Coimbra G., Leitão S.G., de Rezende C.M., Bizzo H.R., Freitas L., Paulino J.V, & Mansano V.D. (2022). Heteromorphic stamens are differentially attractive in Swartzia (Fabaceae). AoB Plants, 14(5), plac041.

+ Open protocol

+ Expand

Histological Evaluation of Liver Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

After scarification, livers were removed and kept in 10% buffered formaldehyde for microscopic inspection. All test individuals in each experimental group received blocks of these livers preserved by formalin-fixed paraffin embedding. Bancroft et al. [32 (link)] described how to fix and stain sections (5 μm thick) using Hematoxylin and Eosin (H&E). Two researchers independently evaluated pathology grades for H&E-stained slides using light microscopy and the Ishak-modified histology activity index system [27 (link)]. The grading method was as follows: 1 for no anomaly, 2 for minor changes (10%), 3 for moderate changes (25%), 4 for significant changes (50%), and 5 for severe/marked alterations (>75%). Images were captured with an Olympus SC30 digital camera and an Olympus BX-46 microscope.

AlGeffari M.A., Mansour D., Ahmed-Farid O., Mohamed Yousef E., Mohamed S.A., Moustafa M.M., Barakat H, & Abd El Ghany K. (2023). Lactiplantibacillus plantarum and Saussurea costus as Therapeutic Agents against a Diabetic Rat Model—Approaches to Investigate Pharmacophore Modeling of Human IkB Kinase and Molecular Interaction with Dehydrocostus Lactone of Saussurea costus. Metabolites, 13(6), 764.

+ Open protocol

+ Expand

Multicolor Immunofluorescence Staining of Splenic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleens were frozen in OCT (Sakura) and cut into 8-μm-thick sections using a cryostat microtome. The sections were dried overnight, blocked with 5% goat sera (Dako), and stained with the following anti-mouse antibodies: B220-APC (RA3-6B2; BD Biosciences), CD138-PE (281-2; BD Biosciences), GL-7-FITC (BD Biosciences), Ly6G-PE (1A8; BioLegend), hCD2-FITC (RPA-2.10, BioLegend), IgG3-APC (R40-82), and CD11b-FITC (M1/70; BD Biosciences). Images were acquired on a confocal laser scanning microscope (Leica; TCS SP5) and processed with Photoshop software (Adobe Systems). For histopathological evaluation of the joints, paws were removed from the mice at the end point (day 61) and fixed by immersion in 4% paraformaldehyde. Paws were then decalcified in 10% ethylenediaminetetraacetate for 2 to 3 wk at room temperature, followed by paraffin embedding and sectioning. Deparaffinized and rehydrated slides were stained with H&E and used to assess joint inflammation and bone degradation (seven slides per joint at 5- and 200-μm intervals between slides). Images were acquired on a Zeiss Axioplan microscope that was equipped with an Olympus SC30 digital camera.

Sedimbi S.K., Hägglöf T., Garimella M.G., Wang S., Duhlin A., Coelho A., Ingelshed K., Mondoc E., Malin S.G., Holmdahl R., Lane D.P., Leadbetter E.A, & Karlsson M.C. (2020). Combined proinflammatory cytokine and cognate activation of invariant natural killer T cells enhances anti-DNA antibody responses. Proceedings of the National Academy of Sciences of the United States of America, 117(16), 9054-9063.

+ Open protocol

+ Expand

Histopathological Evaluation of Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

5-μm-thick paraffin-embedded tumor tissue sections were cut using a Thermo Fisher Scientific HM 340E Electronic Rotary Microtome and then stained with hematoxylin and eosin (H&E). The histopathology of the H&E-stained tumor tissue sections was evaluated by an experienced pathologist, who was blinded to the treatment groups. Histological images were taken by an Olympus (Center Valley, PA) BX43 microscope equipped with an Olympus SC30 digital camera. For mitotic rate analyses in tumor specimens, the number of mitoses in twenty consecutive 400× magnification fields was quantified for each tumor, after which the average number of mitoses per field was calculated to determine the mitotic index for each tumor sample.

Alexander-Bryant A.A., Dumitriu A., Attaway C.C., Yu H, & Jakymiw A. (2015). Fusogenic-oligoarginine peptide-mediated silencing of the CIP2A oncogene suppresses oral cancer tumor growth in vivo. Journal of controlled release : official journal of the Controlled Release Society, 218, 72-81.

+ Open protocol

+ Expand

Seeding and Culturing Huh-7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Huh-7 cells grown in cell culture flasks were washed with PBS, and then trypsinized with Trypsin-EDTA (0.05%), phenol red (Thermo Fisher Scientific, Catalogue # 25300054). Following completion of cell detachment from the flask, growth media (DMEM high glucose (Sigma, Catalogue #D6546) supplemented with 2 mM L-Glutamine (ThermoFisher Scientific, Catalogue #25030081) and 10% Fetal Calf Serum (FCS) (Amimed, Catalogue #2-01F00-I) was added to stop the process. Subsequently the cells were spun in a centrifuge at 200 g in a swinging bucket rotor and washed twice with PBS. Finally the cell pellet was suspended in growth media at a concentration of 5000 cells/μl. 25,000 cells were then used to seed each well of a 6 well plate with or without the basement matrices as prepared earlier. Cells were observed every day, with growth media being changed every alternate day for a total duration of a week. At 1 day and 7 days post seeding, cells were imaged with an Olympus Phase Contrast microscope, and live snapshots were taken with an Olympus CKX41 inverted microscope equipped with a SC 30 digital camera (Olympus). The images were procured on an inverted Phase Contrast microscope with Cell Sens software. All images documented were then processed with OMERO (an initiative of the open microscopy environment https://www.openmicroscopy.org/) for digital zoom.

Ghosh S., Börsch A., Ghosh S, & Zavolan M. (2021). The transcriptional landscape of a hepatoma cell line grown on scaffolds of extracellular matrix proteins. BMC Genomics, 22, 238.

+ Open protocol

+ Expand

Fossil Insect Preservation Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three insect fossils from the Stolleklint Clay and Fur Formation showing various states of preservation were selected for this study: (1) a pair of isolated but three-dimensional beetle elytra (Coleoptera; FUM-N-17627) collected from a calcium carbonate concretion near ash layer +15 in the Fur Formation; (2) a flattened yet fully articulated wasp (Hymenoptera, Ichneumonidae; FUM-N-11263) preserved in a calcium carbonate concretion, collected from the Fur Formation on the Island of Mors; and (3) a compressed but largely articulated damselfly (Odonata, Zygoptera; FUM-N-10904) found in hardened clays of the Stolleklint Clay. All specimens are housed in collections at Museum Salling, Fur Museum, Fur, Denmark, and were photographed in 96% ethanol using an Olympus SZX16 stereo microscope equipped with an Olympus SC30 digital camera prior to ultrastructural and molecular analyses.

Heingård M., Sjövall P., Schultz B.P., Sylvestersen R.L, & Lindgren J. (2022). Preservation and Taphonomy of Fossil Insects from the Earliest Eocene of Denmark. Biology, 11(3), 395.

+ Open protocol

+ Expand

Sphere formation from stem cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

10³ cells were resuspended in 4 ml of STEM medium containing DMEM/F12 (Thermo Fisher Scientific), B27 supplement (Thermo Fisher Scientific, 1X), EGF (Sigma‐Aldrich, 20 ng/ml), and FGF (Sigma‐Aldrich, 10 ng/ml). The cells were then plated in T25 flasks pre‐coated with 1% Noble agar (Difco). Fresh aliquots of medium were added every 3 days, and after 10 days, the spheres were observed and counted on an Olympus CKX41 inverted microscope equipped with a SC 30 digital camera (Olympus) and counted using the ImageJ software.

Ghosh S., Guimaraes J.C., Lanzafame M., Schmidt A., Syed A.P., Dimitriades B., Börsch A., Ghosh S., Mittal N., Montavon T., Correia A.L., Danner J., Meister G., Terracciano L.M., Pfeffer S., Piscuoglio S, & Zavolan M. (2020). Prevention of dsRNA‐induced interferon signaling by AGO1x is linked to breast cancer cell proliferation. The EMBO Journal, 39(18), e103922.

+ Open protocol

+ Expand

Quantifying Tumor-Associated Neutrophils and Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

TANs or TALs were defined as any neutrophils or lymphocytes that were in close proximity to the tumor base in non-invasive neoplasms or between tumor nests in invasive neoplasms. Four fields from the tumor were selected under low magnification (×100), and the neutrophils and lymphocytes were counted at high magnification (× 400) using 2D image analysis software (Olympus CellSense, Tokyo, Japan) on an Olympus BX-46 microscope equipped with an Olympus SC30 digital camera. Care was taken not to count such inflammatory cells in areas of ulceration or erosion. Then, the average number was calculated and scored. TANs and TALs (either CD4 or CD8 T lymphocytes) were identified in the lamina propria just beneath the lower margin of the non-invasive urothelial neoplasm or infiltrated in the cancer nests or stroma.

Hassan W.A., ElBanna A.K., Noufal N., El-Assmy M., Lotfy H, & Ali R.I. (2023). Significance of tumor-associated neutrophils, lymphocytes, and neutrophil-to-lymphocyte ratio in non-invasive and invasive bladder urothelial carcinoma. Journal of Pathology and Translational Medicine, 57(2), 88-94.

+ Open protocol

+ Expand

Tick Specimen Identification and Imaging

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ticks were collected and stored in tubes containing 70–90% ethanol. Samples were visualised using an Olympus SZ61 stereomicroscope (Olympus, Centre Valley, PA, USA) with an external Schott KL 1500 LED (Schott AG Mainz, Germany) light source. Photographs of tick specimens were taken using an Olympus SC30 digital camera (Olympus, Centre Valley, PA, USA) and analysis getIT software (Olympus, Centre Valley, PA, USA). Instar, sex and species were identified using a combination of available morphological keys and species descriptions (Roberts, 1970 ; Jackson et al., 2002 ; Laan et al., 2011 ; Barker and Walker, 2014 ; Kwak et al., 2017 (link)).

Egan S.L., Taylor C.L., Austen J.M., Banks P.B., Northover A.S., Ahlstrom L.A., Ryan U.M., Irwin P.J, & Oskam C.L. (2021). Haemoprotozoan surveillance in peri-urban native and introduced wildlife from Australia. Current Research in Parasitology & Vector-borne Diseases, 1, 100052.

+ Open protocol

+ Expand

Gonadosomatic Index and Cell Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

The gonadosomatic index (GSI) was calculated as an index of the reproductive stage [100 × (GM/BM) (%)] where GM is gonad mass (in grams) and BM is body mass (in grams).
The quantification of anti-BrdU immunostained and TUNEL stained indexes were calculated as the mean value ± SEM of the stained area/total area of 24 randomly distributed optical areas at 200 x magnification. The stained areas were measured by image analysis using a Nikon eclipse E600 light microscope, an Olympus SC30 digital camera (Olympus soft imaging solutions GMBH), and Image Tool 3.00 software (The University of Texas Health Science Center).
Data were analysed by one-way ANOVA and a post hoc test (Tukey Honestly Significant Difference or Waller Duncan) to determine differences between groups (P≤0.05). Normality of the data was previously assessed using a Shapiro-Wilk test and homogeneity of variance was also verified using the Levene test. All data related to sex steroid serum levels and gene expressions did not meet parametric assumptions, and they were subjected to a non-parametric Kruskal-Wallis test, followed by a multiple comparison test. The critical value for statistical significance was taken as P≤0.05. Statistical analyses were conducted using SPSS 12.0 (SPSS, Chicago, IL, USA). All data are presented as mean ± standard error to the mean (SEM).

García-García M., Liarte S., Gómez-González N.E., García-Alcázar A., Pérez-Sánchez J., Meseguer J., Mulero V., García-Ayala A, & Chaves-Pozo E. (2016). Cimetidine disrupts the renewal of testicular cells and the steroidogenesis in a hermaphrodite fish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 189.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!