5 bromo 2 deoxyuridine

Manufactured by Merck Group

Sourced in United States, Germany, Macao, Switzerland

5-bromo-2-deoxyuridine is a synthetic nucleoside that is structurally similar to the DNA base thymidine. It can be used as a research tool to label and track dividing cells.

Automatically generated - may contain errors

Lab products found in correlation

Bromodeoxyuridine (brdu), by Merck Group (21 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (15 mentions) Dmem f12, by Thermo Fisher Scientific (13 mentions) Primary rabbit antibody against brdu, by Abcam (7 mentions) 600 auto biochemical analyzer, by Olympus (7 mentions) Dimethyl sulfoxide (dmso), by Merck Group (5 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (5 mentions) Trypsin, by Thermo Fisher Scientific (5 mentions) Triton x 100, by Merck Group (4 mentions) Transferrin, by Thermo Fisher Scientific (4 mentions) Trypsin edta, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Pancreatin, by Merck Group (3 mentions) Etoposide, by Selleck Chemicals (3 mentions) Penicillin, by Merck Group (3 mentions) Streptomycin, by Merck Group (3 mentions) Nocodazole, by Merck Group (3 mentions) Thymidine, by Merck Group (3 mentions) Sodium selenite, by Thermo Fisher Scientific (3 mentions) Trametinib, by MedChemExpress (3 mentions)

Close spelling variants of this product

5-bromo-2′-deoxyuridine (BrdU; (499 citations) 5-bromo-2′-deoxyuridine (5-BrdU) (5 citations) 5-Bromo-2′-deoxyuridine (BrdU; B5002) (5 citations) 5-Bromo-2′-deoxyuridine (B5002). (3 citations) 5-bromo-2-deoxyuridine (BrdU) solution (2 citations) Mouse anti-5′-bromo-2′-deoxyuridine (BrdU; (2 citations) 5-bromo-2′-deoxyuridine (BrdU) incorporationassay kit (2 citations) BrdU (5′-bromo-2′-deoxyuridine) cell proliferation assay kit (2 citations) Cell Proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) colorimetric kit (2 citations)

172 protocols using 5 bromo 2 deoxyuridine

BrdU Labeling and Temporal Analysis

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On P40, some animals received one daily intraperitoneal injection (150 μg/g body weight) of BrdU (5-bromo-2-deoxyuridine; Sigma) in 0.9% NaCl solution for five consecutive days. Animals were sacrificed either 24 h after the last BrdU injection (on P45) or after 1 month (on P75) (Supplementary Table 1).
On P40, some animals received one daily intraperitoneal injection (150 μg/g body weight) of BrdU (5-bromo-2-deoxyuridine; Sigma) in 0.9% NaCl solution for five consecutive days. Animals were sacrificed either 24 h after the last BrdU injection (on P45) or after 1 month (on P75) (Supplementary Table 1).

Fuchs C., Trazzi S., Torricella R., Viggiano R., De Franceschi M., Amendola E., Gross C., Calzà L., Bartesaghi R, & Ciani E. (2014). Loss of CDKL5 impairs survival and dendritic growth of newborn neurons by altering AKT/GSK-3β signaling. Neurobiology of Disease, 70(100), 53-68.

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Cell Proliferation Assay with BrDU

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Cells plated at 3×10⁵ cells/well and cultured for 24 and 48 h were used to assess proliferation rates. Cultured cells were incubated with 1.5 μg/mL 5-bromo-2′-deoxyuridine (Merck, Darmstadt, Germany) in DMEM/F12 complete medium for 3 h before fixation in 4% paraformaldehyde in PBS for 20 min at room temperature. BrDU was used to detect cells incorporating DNA, using a mouse IgG antibody against BrDU.

Borbely A.U., Sandri S., Fernandes I.R., Prado K.M., Cardoso E.C., Correa-Silva S., Albuquerque R., Knöfler M., Beltrão-Braga P., Campa A, & Bevilacqua E. (2014). The term basal plate of the human placenta as a source of functional extravillous trophoblast cells. Reproductive Biology and Endocrinology : RB&E, 12, 7.

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Osteosarcoma Cell Line Cultivation Protocols

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Osteosarcoma and osteoblastic cell lines used in this study include MNNG/HOS (CLS 300289; Cell Lines Service, GmbH, Eppelheim, Germany), U2OS (CLS-300364; Cell Lines Service, GmbH), 143B (CRL-8303; ATCC, Manassas, VA, USA), MG-63 (CRL-1427; ATCC), Saos-2 (HTB-85; ATCC) and normal human osteoblast cell line hFOB1.19 (CRL-11372; ATCC). MNNG/HOS was cultured in Roswell Park Memorial Institute (RPMI)-1640 medium supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). MG-63 was cultured in DMEM supplemented with 10% FBS. 143B cells was cultured in DMEM supplemented with 10% FBS and 0.015 mg/ml 5-bromo-2′-deoxyuridine (Merck KGaA). U2OS, Saos-2 and hFOB1.19 were cultured in F-12 Medium supplemented with 10% FBS. All the cells were maintained at 37°C in humidified 5% CO₂ incubator.

Chaiyawat P., Sungngam P., Teeyakasem P., Sirikaew N., Klangjorhor J., Settakorn J., Diskul-Na-Ayudthaya P., Chokchaichamnankit D., Srisomsap C., Svasti J, & Pruksakorn D. (2019). Protein profiling of osteosarcoma tissue and soft callus unveils activation of the unfolded protein response pathway. International Journal of Oncology, 54(5), 1704-1718.

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Local Laser Microirradiation of Cells

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For local laser microirradiation, cells were seeded on uncoated, γ-irradiated, gridded microscope dishes (#81166, Ibidi, USA). At 50% confluence, cells were treated with the list of inhibitors see Table 1 and/or pre-sensitized with 10 μM 5-Bromo-2′-deoxyuridine (BrdU;
#11296736001, Merck, Germany) for 16-18 h (see [38, (link)39] (link)). The cells were irradiated using a TCS SP5-X confocal microscope system (Leica, Germany) equipped with the 355-nm laser and 63x oil objective (HCX PL APO, lambda blue) with a numerical aperture (NA) = 1.4. The irradiation conditions were optimized according to [24, (link)40, (link)41] (link).
The cells were maintained under optimal cultivation conditions in an incubation chamber (EMBL, Germany) at 37°C, and the cell culture hood was supplemented with 5% CO2.
Image acquisition for the induction of DSBs was performed with the following settings: 1024 × 1024 pixels, 400 Hz, bidirectional mode, zoom 2. Micro-irradiated cells were monitored for

Legartová S., Pourová J., & Bártová E. (2021). PARP dependent recruitment of RNA methylated at 8-adenosine is linked to base excision repair mechanism.

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Quantifying Hepatocyte Proliferation

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Two hours prior to euthanasia, mice were administered 5′-bromo-2′-deoxyuridine (100 mg/kg; Sigma) i.p. Formalin fixed paraffin embedded (FFPE) liver sections were incubated with primary antibody at 4 °C overnight. Secondary antibody was applied for 1.5 h at room temperature, and nuclei were counterstained with DAPI. Ten high-powered fields (400×) were visualized, and 5-bromo-2′-deoxyuridine (BrdU)-positive hepatocytes were counted on an immunofluorescence microscope (Invitrogen EVOS M5000).

Watkins R., Gamo A., Choi S.H., Kumar M., Buckarma E., McCabe C., Tomlinson J., Pereya D., Lupse B., Geravandi S., Werneburg N.W., Wang C., Starlinger P., Zhu S., Li S., Yu S., Surakattula M., Baguley T., Ardestani A., Maedler K., Roland J., Nguyen-Tran V., Joseph S., Petrassi M., Rogers N., Gores G., Chatterjee A., Tremblay M., Shen W, & Smoot R. (2024). A small molecule MST1/2 inhibitor accelerates murine liver regeneration with improved survival in models of steatohepatitis. PNAS Nexus, 3(3), pgae096.

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Dual-Pulse Labeling of Proliferating Cells

Cited 1 time

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For the labeling of proliferating cells, 5-Bromo-2′-deoxyuridine (BrdU;Sigma-B5022) was dissolved in drinking water (1 mg/ml), and all mouse groups were given access to the water ad libitum throughout the 10 days of the RSDS paradigm^{8 (link)}. For dual-pulse labeling of proliferating populations, 5-ethyl-2’-deoxyuridine (EdU; ThermoFisher Scientific -E10187) was administered i.p. at D17, D19 and D21 (x3 at D17, D19, D21) at a dose of 100 μg/g body weight (8 mg/ml EdU in 0.9 % NaCl), as in^{67 (link)}.

Kokkosis A.G., Madeira M.M., Mullahy M.R, & Tsirka S.E. (2022). Chronic stress disrupts the homeostasis and progeny progression of oligodendroglial lineage cells, associating immune oligodendrocytes with prefrontal cortex hypomyelination. Molecular psychiatry, 27(6), 2833-2848.

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Osteosarcoma and Osteoblast Cell Lines

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Primary OS samples and normal bone samples were collected from 10 patients (6 males and 4 females) via biopsy at Harbin Medical University Cancer Hospital (Harbin, China) between June 2014 and December 2016. The mean age of the patients was 19.3 years (range, 9–67 years). All patients provided written informed consent, and the study was approved by the Ethics Committee of Harbin Medical University. The 143B human osteosarcoma cell line, and human SV40-transfected hFOB 1.19 osteoblasts and 293TN cells were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The 143B cells were cultured in EMEM (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 0.015 mg/ml 5-bromo-2′-deoxyuridine (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO₂ atmosphere. The hFOB 1.19 cells were cultured in D-MEM/F-12 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 0.3 mg/ml G418 (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 34°C in a 5% CO₂ atmosphere. The 293TN cells were cultured in EMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO₂ atmosphere.

Yang H., Li Y., Peng Z, & Wang Y. (2019). Overexpression of miR-20a promotes the progression of osteosarcoma by directly targeting QKI2. Oncology Letters, 18(1), 87-94.

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Primary Neuronal Cell Culture

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5-Bromo-2′-deoxyuridine (BrdU), dimethyl sulfoxide (DMSO), free fatty acid bovine serum albumin (BSA), poly-d-Lysine (PDL), laminin, 4′,6-diamidino-2-phenylindole (DAPI), all-trans-retinoic acid, melatonin, and luzindole were purchased from Sigma-Aldrich (Madrid, Spain). IQM316 was synthesized as previously described^{26 (link)} (IQM316 is referred as compound 16).

Figueiro-Silva J., Antequera D., Pascual C., de la Fuente Revenga M., Volt H., Acuña-Castroviejo D., Rodríguez-Franco M.I, & Carro E. (2018). The Melatonin Analog IQM316 May Induce Adult Hippocampal Neurogenesis and Preserve Recognition Memories in Mice. Cell Transplantation, 27(3), 423-437.

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Mitotic Labeling of Cells Post-Stroke

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5-Bromo-2-deoxyuridine (BrdU, Sigma) is the thymidine analog used for mitotic labeling. To investigate cell proliferation and differentiation, BrdU (50 mg/kg × 2, i.p.) was administered beginning on Day 4 after stroke or sham surgery for 3 consecutive days (N = 6 rats per group). The rats were euthanized 1 day after the final BrdU administration.

Zhang P., Lei X., Sun Y., Zhang H., Chang L., Li C., Liu D., Bhatta N., Zhang Z, & Jiang C. (2016). Regenerative repair of Pifithrin-α in cerebral ischemia via VEGF dependent manner. Scientific Reports, 6, 26295.

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Targeted Laser-Induced DNA Damage Assay

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Cells grown on glass coverslips (Menzel Gläser) were presensitized with 10 μM 5-bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich) for 24 hr at 37°C. Damage was introduced with a 405 nm laser diode (3 mW) focused through a Plan-Apochromat 63x /1.4 oil objective to yield a spot size of 0.5-1 μm using a LSM710 NLO confocal microscope (Zeiss) and the following laser settings: 40% power, 50 iterations, scan speed 12.6 μsec/pixel.

Piquet S., Le Parc F., Bai S.K., Chevallier O., Adam S, & Polo S.E. (2018). The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage. Molecular Cell, 72(5), 888-901.e7.

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