Ifn γ

Manufactured by Mesoscale

Sourced in United States

IFN-γ is a cytokine that plays a key role in the immune system. It is produced by various cell types, including T cells and natural killer cells, and is involved in the regulation of immune responses. IFN-γ has been used in laboratory settings for research purposes.

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Lab products found in correlation

Il 1β, by Mesoscale (4 mentions) Interleukin 6 (il 6), by Mesoscale (4 mentions) Tnf α, by Mesoscale (3 mentions) Interleukin 2 (il 2), by Mesoscale (2 mentions) Il 1α, by Mesoscale (2 mentions) Il 10, by Mesoscale (2 mentions) Tumor necrosis factor (tnf), by Mesoscale (1 mentions) Kc gro, by Mesoscale (1 mentions) Il 12p70, by Mesoscale (1 mentions)

6 protocols using ifn γ

Multiplex Cytokine Profiling

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We performed multiplex assays to measure IL-1β, IL-2, IL-6, TNFα, and IFN-γ purchased from Meso Scale Discovery. All experimental operations were in accordance with standard protocols. R2s of the standard curves for each plate were greater than 0.999.

Bremner J.D., Gurel N.Z., Jiao Y., Wittbrodt M.T., Levantsevych O.M., Huang M., Jung H., Shandhi M.H., Beckwith J., Herring I., Rapaport M.H., Murrah N., Driggers E., Ko Y.A., Alkhalaf M.L., Soudan M., Song J., Ku B.S., Shallenberger L., Hankus A.N., Nye J.A., Park J., Vaccarino V., Shah A.J., Inan O.T, & Pearce B.D. (2020). Transcutaneous vagal nerve stimulation blocks stress-induced activation of Interleukin-6 and interferon-γ in posttraumatic stress disorder: A double-blind, randomized, sham-controlled trial. Brain, Behavior, & Immunity - Health, 9, 100138.

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Multiplex Inflammatory Biomarker Profiling

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A broad panel of inflammatory biomarkers was measured by a multiplex high-sensitivity assay, including interleukin-1β (IL-1β), IL-6, IL-8, IL-10, IL-12, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) (Meso Scale Discovery, Maryland, USA). Serum C-reactive protein (CRP) was measured by immunoturbidometry (Cobas, Roche Diagnostic, Mannheim, Germany).

Habbab K.M., D’Aiuto F., Habbab M.A, & Porter S.R. (2019). Molecular markers relevant to myocardial injury following dental extraction in patients with and without coronary artery disease. BDJ Open, 5, 9.

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Profiling Immune Cytokine Responses

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1.5 × 10⁶ Jurkat, THP1 (ATCC), THP1-Dual or THP1-Dual STING KO cells were treated with 3x GI₅₀ V158411 or 50 μg/mL cGAMP for 24 h. Cells were harvested by centrifugation and lysed in 100 μL ice cold lysis buffer on ice for 30 min. Cell debris was pelleted and 25 μL of the supernatant added to a 10 spot U-PLEX biomarker plate pre-conjugated with anti-TNF-α, MCP-3, IL-6, IL-22, IL-18, IL-1β, IL-1α, IFN-γ, IFN-β and IFN2a capture antibodies (MesoScale Discovery, Rockville, MD). The plate was developed according to the manufacturer’s instructions and read using a SECTOR Imager 2400.

Brooks T., Wayne J, & Massey A.J. (2021). Checkpoint Kinase 1 (Chk1) inhibition fails to activate the Stimulator of Interferon Genes (STING) innate immune signalling in a human coculture cancer system. Molecular Biomedicine, 2, 19.

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Cytokine Analysis in Maternal Plasma

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Maternal plasma samples were analysed for SASP using the Human U-Plex kit IL-6, IL-8, IL-13, IL-1α, IFN-γ, MCP-1, and MIP-1α (MesoScale Discovery, Rockville, MD, USA; K15067L) and the Human MMP3 Ultra-sensitive kit (MesoScale Discovery, Rockville, MD, USA; K15034CL). All samples were prepared using the manufacturer’s instructions and plates analysed on the Meso QuickPlex SQ 120 platform. Results were generated as calculated concentration means on the Mesoscale (MSD) Discovery Workbench 4.0 assay analysis software. The MSD analysis software determines individual cytokine concentrations from electro chemiluminescent signals via backfitting to the calibration curve.

Manna S., Mc Elwain C.J., Maher G.M., Giralt Martín M., Musumeci A., McCarthy F.P, & McCarthy C. (2023). Heterogenous Differences in Cellular Senescent Phenotypes in Pre-Eclampsia and IUGR following Quantitative Assessment of Multiple Biomarkers of Senescence. International Journal of Molecular Sciences, 24(4), 3101.

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Intradermal LPS Administration Safety and Cytokine Response

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Safety and tolerability were monitored by tracking adverse events, measuring vital signs, and standard laboratory tests (i.e. haematology) at 3, 6, 10, 24 and 48 hours after LPS administration. Circulating cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, IFN‐γ and TNF; Meso Scale Discovery, Rockville, Maryland, USA) were measured in blood samples to detect a possible systemic effect of intradermal LPS administration. The blood samples for cytokine analysis were analysed in 2 batches with each having a different dilution, therefore the lower limit of quantitation (LLOQ) of the samples was the following: IL‐1β 0.298 or 0.745 pg/mL; IL‐6 1.52 or 3.81 pg/mL; IL‐8 1.25 or 3.12 pg/mL; IL‐10 0.702 or 1.76 pg/mL; IFN‐γ 10 or 25 pg/mL; and TNF 0.760 or 1.90 pg/mL.

Buters T.P., Hameeteman P.W., Jansen I.M., van Hindevoort F.C., ten Voorde W., Florencia E., Osse M., de Kam M.L., Grievink H.W., Schoonakker M., Patel A.A., Yona S., Gilroy D.W., Lubberts E., Damman J., Feiss G., Rissmann R., Jansen M.A., Burggraaf J, & Moerland M. (2021). Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers. British Journal of Clinical Pharmacology, 88(2), 680-690.

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BALF Cytokine Profiling via MSD

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The BALF supernatant samples were analysed using MSD pro-inflammatory panel V-Plex including the following analytes: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO and TNF-α (Meso Scale Diagnostics, Rockville, MD). The analysis was performed according to the manufacturer’s instruction.

Wronski S., Dannenmaier J., Schild S., Macke O., Müller L., Burmeister Y., Seilheimer B, & Müller M. (2018). Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS ONE, 13(4), e0195822.

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