HAPNPs, CsNPs, and CurNPs were examined for morphology using the transmission electron microscope, TEM (JEOL JEM 2100Plus operated at 80 kV, Japan), where samples were placed on carbon-coated copper grids and left 5 min to dry at room temperature; the extra solution was distanced by blotting paper. The JEOL JEM 2100Plus is a versatile TEM for both large-scale 2D screening as well as tomography. It runs Serial EM and thus is capable of automated multiposition acquisitions. Depending on the type and thickness of the specimen, the acceleration voltage can be chosen from 80 to 200 kV.
Jem 2100 plus
Manufactured by JEOL
Sourced in Japan, United States, United Kingdom, Germany
The JEM-2100 Plus is a high-performance transmission electron microscope (TEM) designed for advanced materials analysis. It features a LaB6 electron source, providing high-quality electron beam performance. The JEM-2100 Plus is capable of delivering a maximum accelerating voltage of 200 kV and offers a wide range of imaging and analytical capabilities for researchers and scientists working in materials science, nanotechnology, and other related fields.
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Lab products found in correlation
Zetasizer nano z, by Malvern Panalytical (8 mentions)
D8 advance, by Bruker (7 mentions)
Oneview 4k camera, by Ametek (7 mentions)
Escalab 250xi, by Thermo Fisher Scientific (5 mentions)
Uranyl acetate, by Merck Group (5 mentions)
S 4800, by Hitachi (5 mentions)
Apreo s, by JEOL (4 mentions)
Irtracer 100, by Shimadzu (4 mentions)
Jsm it200, by JEOL (4 mentions)
Nano zs90, by Malvern Panalytical (4 mentions)
Miniflex 600, by Rigaku (4 mentions)
Lead citrate, by Merck Group (3 mentions)
K alpha, by Thermo Fisher Scientific (3 mentions)
Formvar coated copper grids, by Science Services (3 mentions)
Jsm 7800f, by JEOL (3 mentions)
Ifs 66v s spectrometer, by Bruker (3 mentions)
Su8010, by Hitachi (3 mentions)
Em uc6, by Leica (3 mentions)
Zetasizer nano zs90, by Malvern Panalytical (3 mentions)
Temcam xf416, by TVIPS (3 mentions)
216 protocols using jem 2100 plus
1
Transmission Electron Microscopy for Nanoparticle Characterization
2
Characterization of Li and Na Particles Using Electron Microscopy
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Powder X-ray diffraction patterns were obtained using a Bruker AXS D8 Advance diffractometer with a Cu Kα source (λCu Kα = 1.54 Å).
TEM experiments were performed on JEOL JEM-2100 Plus (200 kV) and double aberration-corrected JEOL GrandArm (300 kV). To be specific, HRTEM images in Figs.3 –4 , EFTEM images and EELS were obtained on JEOL GrandArm. The Gatan Oneview IS camera enabled fast in situ data acquisition. Other TEM results were obtained on JEOL JEM-2100 Plus. Cryogenic experiments were carried out with Gatan double tilt cooling holder (Model 636) which can sustain a low-temperature environment at −178 °C. In situ heating experiments were carried out with Protochips (Fusion 350) in situ heating holder. Lithium whisker contact experiments were carried out with an in situ STM tip holder (PicoFemto). AC-HRTEM images of lithium and sodium particles were collected under the negative spherical aberration (CS) imaging (NCSI) condition. Average background subtracted filtering was carried out based on the script from D. R. G. Mitchell and method by Kilaas35 (link).
TEM experiments were performed on JEOL JEM-2100 Plus (200 kV) and double aberration-corrected JEOL GrandArm (300 kV). To be specific, HRTEM images in Figs.
3
Advanced Techniques for Hybrid Material Characterization
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High-resolution scanning electron microscopy (HR-SEM, Thermoscientific Apreo S) and transmission electron microscopy (TEM, JEM-2100 Plus, JEOL) were utilized to predict the morphological, surface properties, and chemical content, and the selected area electron diffraction (SAED) pattern of produced h-BN, and PTh/h-BN hybrid. Energy dispersive spectroscopy (EDS-JEM-2100 Plus, JEOL, and Thermoscientific Apreo S) was utilized for elemental and chemical composition. The crystalline nature of the synthesized compound was examined by using X-ray diffraction spectroscopy (XRD system-X'pert powder, with Cu-Kα radiation (λ = 0.154 nm) Malvern Panalytical, United Kingdom) at 45 kV (tension) and 40 mA (current) with 0.02° per step scan and 1° per min speed. The functional groups of the synthesized materials were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR) technique, (FT-IR spectrophotometer, Shimadzu, IR Tracer 100). The cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and amperometry (i–t curve) studies were carried out using CHI electrochemical workstation (CHI 760E, USA). All the measurements were performed at room temperature using a three-electrode setup. The counter, reference, and working electrodes were platinum wire (Pt), Ag/AgCl (3 M KCl), and glassy carbon electrode (GCE), respectively.
4
Ultrastructural Analysis of SARS-CoV-2
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Purified SARS-CoV-2 viral suspensions were fixed in 2.5% glutaraldehyde and allowed to adsorb onto a formvar carbon-coated grid for a few minutes before being stained with 1% phosphotungstic acid for 1 min. The excess fluid was blotted and the grid left to dry before viewing under a transmission electron microscope JEOL JEM 2100 Plus (Japan Electron Optics Laboratory Co. Ltd. Tokyo). Images were captured digitally with a digital camera TVIPS (Tietz Video and Image Processing Systems GmbH. Gauting, Germany).
5
Ultrastructural Analysis of SARS-CoV-2 in Cells
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Transmission electron microscopy (TEM) was performed on cultured cells and autopsy lung specimens using standard procedures. The ultrastructural analysis was performed on 4 out of 20 cases considered for this study, which resulted with positive SARS-CoV-2 PCR test on the lung tissue. Cultured cells and small pieces of tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer, for 4 h at 4 °C. Post-fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described47 (link),48 (link). Ultrathin sections were stained with 2% uranyl acetate and observed under a transmission electron microscope JEOL JEM 2100 Plus (Japan Electron Optics Laboratory Co. Ltd. Tokyo, Japan). Images were captured digitally with a digital camera TVIPS (Tietz Video and Image Processing Systems GmbH. Gauting, Germany).
LD number and LD-mitochondria contacts were counted by analyzing at least 30 cells per each condition, at the electron microscope, assessed in blind by two authors.
LD number and LD-mitochondria contacts were counted by analyzing at least 30 cells per each condition, at the electron microscope, assessed in blind by two authors.
6
Characterization of Engineered CNPs
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The generated CNPs was coated by gold sputter (SPI-Module), then the morphology, size, and construction were inspected by SEM (model JEOL-JSM-IT200) at 20 kV. Another morphological examination of CNPs was performed by TEM. Samples of the generated CNPs were inspected with the TEM unit (TEM; JEM-2100 Plus, JEOL Ltd., Japan).
7
Characterizing Lip-DT Vesicle Morphology
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The vesicle morphology of the Lip-DT was characterized with a HR-SEM (Thermo scientific Apreo S) operating at 15.00 kV and HR-TEM (JEOL-JEM-2100 Plus) operating at 200 kV. For HR-SEM analysis 20 μL of the Lip-DT was drop casted on a clean glass coverslip and dried at RT. For HR-TEM analysis a 10 μL drop of a Lip-DT was placed on a 200-mesh copper grid and covered by carbon-stabilized Formvar® film. After 1 min, excess fluid was removed from the grid. The morphology and fluorescent nature of the vesicles were visualized using a Leica DM6 Fluorescent Microscope with Cryostat. Dynamic light scattering analysis (Zetasizernano ZS, Malvern Instruments, Malvern, U.K.) was carried out to find the size distribution and surface charge of the Lip-DT and PCDA/DMPC-based liposomes. Dynamic light scattering (DLS) measurements were performed using the samples diluted to approximately 0.05 mM.17 (link)
8
Comprehensive Characterization of Catalytic Materials
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The crystal phase formation of the as-prepared samples was determined using an X-ray diffractometer (XRD) (Bruker D8 Advance, Germany) with Cu Kα radiation (0.15406 nm, 40 kV, 40 mA). The morphologies of the samples were examined by scanning electron microscope (SEM) (Philips XL30 SFEG, the Netherlands) and HRTEM analyses (JEOL JEM-2100 Plus, Japan, operated at 200 kV). The elemental compositions of the samples were determined by energy-dispersive X-ray spectroscopy (EDS) analysis (Philips XL30 SFEG, Netherlands). Fourier transform infrared (FT-IR) analysis (Perkin Elmer Spectrum 100, Germany) was performed to determine the functional groups of the samples. The chemical states of the sample were identified using X-ray photoelectron spectrometer (XPS) analysis (Thermo Scientific Escalab 250Xi+, USA). N2 adsorption–desorption analysis, using the BELSORP model of Mini II (Japan), was used to explore the samples’ specific surface area and pore structure. The differential reflectance spectroscopy (DRS) of the samples was recorded using a Shimadzu UV-2600 spectrophotometer (Japan). The leaching concentrations of lanthanum, zinc, and iron ions were determined by ICP-MS using an Agilent 7800 (USA). To determine the by-products generated during the sonocatalytic degradation of metribuzin, GC–MS analysis was performed using an Agilent 6890 N (USA).
9
Transmission Electron Microscopy (TEM) Imaging
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Cells were fixed in fixation buffer (2% glutaraldehyde, 2.5% sucrose, 3 mM CaCl2, 100 mM HEPES‐KOH pH 7.2) at room temperature for 30 min and 4°C for 30 min. Samples were washed three times in 1% osmium tetroxide, 1.25% sucrose, and 1% potassium ferrocyanide in 0.1 M sodium cacodylate buffer. After dehydration in alcohol gradient series and propylene oxide, the tissue samples were embedded. Ultrathin sections were cut on a diamond knife (Diatome, Biel, Switzerland) on a Leica ultramicrotome and placed on copper grids (Science Services, 100mesh). Sections were stained with uranyl acetate (Plano, 1.5%) and lead citrate (Sigma) and examined with an electron microscope (JEM 2100 Plus, JEOL) with a OneView 4 K camera (Gatan) with DigitalMicrograph software at 80 kV.
10
TEM Analysis of Material Composition
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EDX analysis was performed using TEM “JEM-2100 Plus, JEOL Ltd., Japan; at the Central Laboratory, City of Scientific Research and Technological Applications, Alexandria, Egypt”.
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