Anti myc cy3

Manufactured by Merck Group

Anti-Myc-Cy3 is a fluorescently labeled antibody that specifically binds to the Myc epitope. It can be used for the detection and visualization of Myc-tagged proteins in various biological applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti ha fitc, by Merck Group (1 mentions) Confocal laser scanning microscope, by Zeiss (1 mentions) Anti ha, by Thermo Fisher Scientific (1 mentions) Anti lgr5, by Abcam (1 mentions) Anti ha alexa 488, by Cell Signaling Technology (1 mentions) Duolink in situ pla probe, by Merck Group (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Anti flag, by Merck Group (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Chloroquine, by Merck Group (1 mentions) Mouse anti m13 antibody, by GE Healthcare (1 mentions) Vectashield mounting media, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Mouse Cy3 conjugated anti-c-Myc antibody [9E10] (3 citations) Anti-c-myc-Cy3 antibody (3 citations) Anti-c-myc-Cy3 (2 citations) Anti-Myc (274 citations)

3 protocols using anti myc cy3

Immunofluorescence Staining of Tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formaldehyde-fixed, permeabilized cells cultured on glass coverslips were incubated with primary antibodies, anti-myc-Cy3 (Sigma), anti-Flag produced in rabbit (Sigma), and anti-HA-FITC (Sigma) for 1 hour at 37°C, followed by incubation with secondary anti-rabbit-Cy5 (KPL) and DAPI. Cells were analysed on a confocal laser scanning microscope (Karl Zeiss).

Tamura R.E., Paccez J.D., Duncan K.C., Morale M.G., Simabuco F.M., Dillon S., Correa R.G., Gu X., Libermann T.A, & Zerbini L.F. (2016). GADD45α and γ interaction with CDK11p58 regulates SPDEF protein stability and SPDEF-mediated effects on cancer cell migration. Oncotarget, 7(12), 13865-13879.

+ Open protocol

+ Expand

Comprehensive Antibody Characterization for Wnt Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant full-length RSPO proteins (RSPO1–4) were purchased from R&D Systems, and human recombinant RSPO1 furin domain (aa 1–146, C-terminal His-tag) was from Sino Biological. For western blot and immunoprecipitation, anti-HA (Invitrogen cat #71–5500 or Cell Signaling cat #2367), anti-Myc (Cell Signaling cat #2276), anti-phosphorylated and total LRP6 (Cell Signaling cat #2568 and #3395), anti-β-actin (Cell Signaling cat #4970), anti-LGR4 (7E7) (7 (link)), anti-FLAG (Sigma cat #F7425), anti-LGR5 (Abcam cat #ab75850), anti-RNF43 (SC37) (31 ), and anti-GAPDH (Cell Signaling cat #2118) were used. For ICC, anti-HA-Alexa 488 (Cell Signaling cat #2350), anti-Myc-Cy3 (Sigma cat #C6594s), TO-PRO-3 Iodide (Thermo Fisher cat #T3605), and anti-human-Alexa 488 or –Alexa 555 (Invitrogen cat #A11013 and #A21433) were used. Anti-pan FZD antibody (18R5) were prepared as described before (46 (link)). For PLA and IP, anti-LGR5 (8F2) and anti-LGR4 (8D2) antibodies were produced as described (28 (link), 35 ). Duolink^® In Situ PLA probes (anti-mouse PLUS and MINUS, anti-rabbit PLUS and MINUS, and anti-human PLUS and MINUS) and Duolink^® In Situ PLA detection reagents green were purchased from Sigma. For TR-FRET, Lance Europium-labeled anti-6X His antibody, Lance Ultra Ulight^™-anti-6xHis, and ULight anti-Myc were purchased from PerkinElmer, and anti-HA-Alexa 647 were from Invitrogen.

Park S., Wu L., Tu J., Yu W., Toh Y., Carmon K.S, & Liu Q.J. (2020). Unlike LGR4, LGR5 potentiates Wnt–β-catenin signaling without sequestering E3 ligases. Science signaling, 13(660), eaaz4051.

+ Open protocol

+ Expand

Visualization of Internalized Phage Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols

For staining of internalized phage particles on live cells, approximately 70,000 HMEC-1 cells were seeded per well in a 4-well permanox chamber slide a day in advance. One hour prior to addition of phage 100 μM chloroquine (Sigma-Aldrich) was added to the media. The cells were moved to room temperature and phage or soluble antibody was added directly to the media and incubated for 30 minutes. The cells were washed one time in fresh medium and moved to 37 °C for 2 hours. After incubation the cells were washed 3 times with EGM and 3 times with PBS and fixed in 2% PFA for 10 minutes at room temperature, residual PFA was removed by PBS. The cells were permeabilized using 0.1% TritonX100 for 15 minutes at RT. The permeabilized cells were blocked 1 hour in 2% mPBS. The cells were incubated 2 hours with mouse anti-M13 antibody (GE Healthcare) (1:4000) in 2% mPBS or anti-Myc-Cy3 (Sigma-Aldrich) (1:1000) For phage staining anti-M13, this was followed by 2 times wash in PBS and incubation with alexa-488 conjugated goat anti-mouse antibody (GE Healthcare) (1:500) in 2% mPBS for minimum 1 hour. The cells were washed 2 times in PBS and mounted using Vectashield mounting media (Vectorlabs).

Mandrup O.A., Lykkemark S, & Kristensen P. (2017). Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy. Scientific Reports, 7, 42230.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!