Mature cotyledonary SEs of Hevea (Reyan 73397), 1.5 cm in size, were selected from in vitro-grown cultures, which were provided by the Innovation Base for production of Natural Rubber New Planting Material, Danzhou, China. The embryos were then cultured on a Murashige and Skoog (MS) [46 (link)]-based embryogenesis medium (MSE) [4 (link)] with a slight modification (4.44 μM 6-benzyladenine, 13.9 μM Kinetin (KT),1.44 μM Gibberellic acid, 0.27 μM 2,4 D 2,4-Dichlorophenoxyacetic acid (2,4-D), 70 g/L sucrose, 50 ml coconut water, 1 g charcoal and 2.2 g/L phytagel) in a 100 mm × 20 mm Petri dish (Sigma, St. Louis, MO, USA).
Petri dish
Manufactured by Merck Group
Sourced in United States
A Petri dish is a shallow, circular glass or plastic dish used in laboratories for culturing microorganisms, such as bacteria and fungi. It is a standard piece of laboratory equipment designed to provide a controlled environment for the growth and study of these microscopic organisms.
Automatically generated - may contain errors
Lab products found in correlation
Licf3so3, by Merck Group (1 mentions)
Orbitrap lumos, by Thermo Fisher Scientific (1 mentions)
Chitosan oligosaccharide lactate, by Merck Group (1 mentions)
Mueller hinton agar (mha), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Zinc sulfate heptahydrate, by Merck Group (1 mentions)
Centrifuge tube, by Eppendorf (1 mentions)
Phosphate buffered saline solution 1xpbs, by Merck Group (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Micro ct, by Bruker (1 mentions)
8 protocols using petri dish
1
Hevea Somatic Embryogenesis Protocol
2
Synthesis of PVA-based Solid Polymer Electrolytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sigma Aldrich (Kuala Lumpur, Malaysia) supplied the Poly (vinyl alcohol) (PVA) (averageMw85,000–124,000,87%–89%) powder material. In the present work, solid polymer electrolytes based on PVA were synthesised by a facile conventional solution cast technique. The procedure includes dissolution of one gram of PVA in 50 mL of distilled water at 90 °C. The solution was stirred continuously with aid of magnetic stirrer for several hours until the PVA powder was completely dissolved, to obtain homogeneous viscous solution. Afterwards, the PVA solution was left to cool down to room temperature. Subsequently, 10 wt.% of lithium trifluoromethanesulfonate (LiCF3SO3) (Sigma-Aldrich, Kuala Lumpur, Malaysia) [CAS Number 33454-82-9, Molecular Weight = 156.01 g/mol] was added to the solution to make it alkaline solution of PVA:LiCF3SO3 polymer electrolyte. Then, the mixture was stirred continuously until a homogeneous solution was obtained. Ultimately, after casting in Petri dish (90 mm × 15 mm, Sigma-Aldrich, Kuala Lumpur, Malaysia), the solution was left to dry to form a film at room temperature. The films produced were then put into desiccators for extra drying and moisture elimination.
3
Fly Survival Assay with Silica
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In each assay, groups of ten six to 10 days old flies were collected on ice and incubated in a petri dish containing silica (Sigma Aldrich) at 22°C. The petri dish was sealed with parafilm during the experiment. The experiment was repeated at least three times. Control flies survive for several hours in the petri dish without silica.
4
Proteomic Profile of HIIT Serum Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCFs (2.1 × 105 for 5–10 passages) were inoculated in a Petri dish with a 60-mm diameter (Sigma‒Aldrich) and then treated as described for the above cell behaviour assays. After 24 h of incubation in pre- and post-HIIT serum, cells were collected and homogenized in lysis buffer (8 M urea in 50 mM triethyl ammonium bicarbonate buffer, pH 8). The prepared sample was further analysed by nano-LC–ESI–MS on an Orbitrap LUMOS mass spectrometer (Thermo Fisher Scientific Inc.) for label-free quantification of the protein profile (see Additional file 2 for the proteomic study procedure).
5
Embryoid Body Formation and Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EBs were generated using the three-dimensional hanging drop (HD) technique. For this, mESCs (1000 cells/10 µL) were suspended in the EB differentiation medium and placed as a drop (10 µL) on the inner side of the lid of a petri dish (Sigma-Aldrich, NZ). Around 40–50 drops were evenly placed per dish (Fig. 1 B). The base of the petri dish was filled with 10 mL of sterile 1X phosphate buffered saline (PBS) as a source of humidity to the cell cluster in the hanging drops. The lid was then inverted, closed and cultured at 37 °C in a 5% CO2 incubator. Spherical EBs were visibly formed by day 2. The EBs on day 3 were gently flushed and plated on uncoated petri dishes in EB differentiation medium supplemented with AA and Wi followed by treatment with RAi as shown in Fig. 1 B.
6
Chitosan-HNT Composites for Antimicrobial Applications
7
Isolation of Venom Glands from Tarantula
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before venom glands were collected, T. sinensis adult females were briefly (a few minutes) anesthetized on ice and subsequently placed in a phosphate-buffered saline solution (1X PBS) (Sigma, St. Louis, MO, USA) in a Petri dish (Sigma, St. Louis, MO, USA). A stereo microscope (Nikon, Tokyo, Japan) was used for dissections. The entire reproductive apparatus was removed with a pair of forceps and placed in a drop of 1X PBS solution. Subsequently, the venom glands were isolated and placed in a centrifuge tube (Eppendorf, Hamburg, Germany) containing TRI Reagent (Sigma, St. Louis, MO, USA) for the following RNA extraction and on slides for the subsequent microscope observations (Sigma, St. Louis, MO, USA).
8
Characterization of Porous KH Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microstructure of 20% (w/v) KH was assessed by scanning electron
microscopy (SEM; JEOL Ltd, Tokyo, Japan) to determine pore size and
micro-computed tomography (micro-CT; Bruker, Belgium) for porosity. The KH
was injected into cylindrical silicone tubing (60 × 10 mm) placed inside a
Petri dish (60 × 15 mm; Sigma, St. Louis, MO, USA) at −20°C and lyophilized.
Representative cylindrical scaffolds (cross-sectioned) were used for
analysis. The pore size was determined by using the free hand tool of Image
analysis software (ImageJ; National Institutes of Health, Bethesda, MD,
USA).
microscopy (SEM; JEOL Ltd, Tokyo, Japan) to determine pore size and
micro-computed tomography (micro-CT; Bruker, Belgium) for porosity. The KH
was injected into cylindrical silicone tubing (60 × 10 mm) placed inside a
Petri dish (60 × 15 mm; Sigma, St. Louis, MO, USA) at −20°C and lyophilized.
Representative cylindrical scaffolds (cross-sectioned) were used for
analysis. The pore size was determined by using the free hand tool of Image
analysis software (ImageJ; National Institutes of Health, Bethesda, MD,
USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!