Cy 09

Manufactured by Selleck Chemicals

Sourced in United States, China

CY-09 is a laboratory centrifuge designed for separating and concentrating biological samples. It features a compact design and can accommodate a variety of sample sizes and volumes. The centrifuge operates at adjustable speeds to enable efficient separation of components within the sample.

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Lab products found in correlation

Gemcitabine, by AbMole (1 mentions) Pm150210, by Procell (1 mentions) Gemcitabine, by Selleck Chemicals (1 mentions) Wnt c59, by Selleck Chemicals (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Autophagy inhibitor 3 ma, by Merck Group (1 mentions) Fps zm1, by Selleck Chemicals (1 mentions) Ly294002, by Selleck Chemicals (1 mentions) Vx 765, by Selleck Chemicals (1 mentions) Anti ck19, by Abcam (1 mentions) Anti vimentin, by Abcam (1 mentions)

4 protocols using cy 09

Exploring TNBC Chemoresistance Mechanisms

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TNBC cell lines (TNBCC), MDA-MB-231 and MDA-MB-468 were purchased (ATCC, CA, U.S.A.). The Gemcitabine-resistance cell lines (GRC), MDA-MB-231-R and MDA-MB-468-R were produced by treating TNBCC with increasing Gemcitabine concentrations (0.1–15 nm) for 12 months (GI50 < 7.0) [18 (link)]. Gemcitabine was bought from AbMole (Beijing, China). Cells were cultured in DMEM (PM150210) containing 4500 mg/l glucose, 10% fine FBS (164220-100) and 100 U/ml penicillin–streptomycin (PB180122) in 5% CO₂, 95% O₂ condition at 37°C (Procell, Wuhan, China).
Each cell line was also incubated with NLRP3 agonist Nigericin sodium salt (NSS) and antagonist CY-09 separately (Selleck, Shanghai, China) to differentiate NLRP3 expression in both TNBCC and GRC. Wnt inhibitor Wnt-C59 was added into subgroups of GRC so as to inactivate signaling pathway (Selleck, Shanghai, China).
All the cell lines were exposed to the 0, 1, 3, 5 nM Gemcitabine, respectively, for 72 h for the following assays.

Zheng Q., Yao D., Cai Y, & Zhou T. (2020). NLRP3 augmented resistance to gemcitabine in triple-negative breast cancer cells via EMT/IL-1β/Wnt/β-catenin signaling pathway. Bioscience Reports, 40(7), BSR20200730.

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Preparation of Autophagy and HMGB1 Modulators

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TMZ (No. PHR1437, Sigma) was dissolved in dimethyl sulfoxide (DMSO) at a 100 mM stock concentration and stored at -20 °C. Autophagy inhibitor 3-MA (No. M9281, Sigma) and LY294002 (No. S1105, Selleck Chemicals) were dissolved to 10 mM and 100 μM as stock solutions. CY-09 (No. S5774, Selleck Chemicals) and FPS-ZM1 (No. S8185, Selleck Chemicals) were at 5 mM and 100 μM as stock concentrations. Recombinant human HMGB1 (rhHMGB1, No.1690-HMB-050) was purchased from R&D Systems and recombinant mouse HMGB1 (rmHMGB1, Cat. 50913-M01H) was from Sino Biological.

Li Z., Fu W.J., Chen X.Q., Wang S., Deng R.S., Tang X.P., Yang K.D., Niu Q., Zhou H., Li Q.R., Lin Y., Liang M., Li S.S., Ping Y.F., Liu X.D., Bian X.W, & Yao X.H. (2022). Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarization. Journal of Experimental & Clinical Cancer Research : CR, 41, 74.

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NLRP3 Inhibitor CY-09 Neuroprotection Protocol

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A single dose of NLRP3 inhibitor CY-09 (40 mg·kg⁻¹, i.p., Selleckchem, USA) was administered 1 h before MCAO surgery as described previously [20 (link)]. In cultured neurons, CY-09 (10 μM) was pre-incubated for 0.5 h before OGD. Then, neurons were incubated with CY-09 (10 μM) for 24 h after reoxygenation. Vehicle animals were treated with equivalent normal saline (i.p.) for comparison.

Sun R., Peng M., Xu P., Huang F., Xie Y., Li J., Hong Y., Guo H., Liu Q, & Zhu W. (2020). Low-density lipoprotein receptor (LDLR) regulates NLRP3-mediated neuronal pyroptosis following cerebral ischemia/reperfusion injury. Journal of Neuroinflammation, 17, 330.

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Immunohistochemical Characterization of hESCs

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Cell suspensions (1 × 10⁶ cells/mL) were inoculated in a six-well plate, covered with sterile cover glass, and incubated for 24 h. When cell confluence reached 80%, cells were fixed in 4.0% paraformaldehyde and permeated using 0.1–0.2% polyethylene glycoloctylphenylether. After incubation for 15 min, 5.0% normal goat abandoned blood serum was added. Anti-CK19 (1:50; Abcam, Cambridge, UK) and anti-vimentin (1:50; Abcam) were added and a negative control (PBS instead of primary antibody) was set up simultaneously; all samples were placed in wet box at 4 °C overnight. After rewarming on the next day, diluted secondary antibody was added, incubated at 37 °C for 15 min, and dyed using the SP method (i.e., adding horseradish-labeled chain enzyme lactalbumin working solution). The neutral gum was then sealed and observed under a light microscope.Immunohistochemistry analysis showed that CK19 was negative and vimentin was positive in hESCs and hEM15A. CY09 and VX-765 were purchased from Selleck (Shanghai, China).

Huang Y., Li R., Hu R., Yao J, & Yang Y. (2022). PEG2-Induced Pyroptosis Regulates the Expression of HMGB1 and Promotes hEM15A Migration in Endometriosis. International Journal of Molecular Sciences, 23(19), 11707.

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