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Mantra multispectral imaging platform

Manufactured by PerkinElmer
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The Mantra multispectral imaging platform is a laboratory equipment product designed for spectral imaging. It captures high-resolution images across multiple wavelength bands, enabling detailed analysis of various samples.

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Lab products found in correlation

Vectra 3, by PerkinElmer (2 mentions) Inform 2.3 image analysis software, by PerkinElmer (2 mentions) Inform 2, by PerkinElmer (1 mentions)

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Mantra (11 citations) Mantra imaging platform (2 citations)

4 protocols using mantra multispectral imaging platform

1

Multispectral Imaging for Tumor Analysis

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Image acquisition was performed using the Mantra multispectral imaging platform (PerkinElmer, Hopkinton, MA). Areas with non-tumor or residual normal tissue were excluded from the analysis. Representative regions of interest were chosen by the pathologist (S.J.R.), and 3-5 fields of view (FOVs) were acquired at 20x resolution as multispectral images. After image capture, the FOVs were spectrally unmixed and then analyzed using supervised machine learning algorithms within Inform 2.4 (PerkinElmer). Thresholds for positive staining and the accuracy of phenotypic algorithms were optimized and confirmed for each case.
Cader F.Z., Hu X., Goh W.L., Wienand K., Ouyang J., Mandato E., Redd R., Lawton L.N., Chen P.H., Weirather J.L., Schackmann R.C., Li B., Ma W., Armand P., Rodig S.J., Neuberg D., Liu X.S, & Shipp M.A. (2020). A Peripheral Immune Signature of Responsiveness to PD-1 Blockade in Patients with Classical Hodgkin Lymphoma. Nature medicine, 26(9), 1468-1479.
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2

Multispectral Imaging for Tumor Analysis

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Image acquisition was performed using the Mantra multispectral imaging platform (Vectra 3, PerkinElmer, Hopkinton, MA) as previously described 34 (link). Areas with non-tumor or residual normal tissue (i.e. residual lymph node) were excluded from the analysis. Representative regions of interest were chosen by the pathologist, and 5–7 fields of view (FOVs) were acquired at 20× resolution as multispectral images. Image Analysis was performed using the Inform 2.3 Image Analysis Software (PerkinElmer, Hopkinton, MA).
Cañadas I., Thummalapalli R., Kim J.W., Kitajima S., Jenkins R.W., Christensen C.L., Campisi M., Kuang Y., Zhang Y., Gjini E., Zhang G., Tian T., Sen D.R., Miao D., Imamura Y., Thai T., Piel B., Terai H., Aref A.R., Hagan T., Koyama S., Watanabe M., Baba H., Adeni A.E., Lydon C.A., Tamayo P., Wei Z., Herlyn M., Barbie T.U., Uppaluri R., Sholl L.M., Sicinska E., Sands J., Rodig S., Wong K.K., Paweletz C.P., Watanabe H, & Barbie D.A. (2018). Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Nature medicine, 24(8), 1143-1150.
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3

Multiplex Immunohistochemistry and Multispectral Imaging

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Staining was performed using BOND RX fully automated stainers (Leica Biosystems). The target antigens, antibody clones and dilutions for markers, as well as the details of controls are listed in Supplementary Table 6. Image acquisition was performed using the Mantra multispectral imaging platform (Vectra 3, PerkinElmer). Areas with non-tumour or residual normal tissue (that is, residual lymph node) were excluded from the analysis. Representative regions of interest were chosen by the pathologist, and multiple fields of view were acquired at 20× resolution as multispectral images. Cell identification was performed as described39 (link), using supervised machine learning algorithms within Inform 2.3 (PerkinElmer). Thresholds for ‘positive’ staining and the accuracy of phenotypic algorithms were optimized and confirmed by the pathologist (S.J.R.) for each case. The protocol is described in full in the Supplementary Information.
Keskin D.B., Anandappa A.J., Sun J., Tirosh I., Mathewson N.D., Li S., Oliveira G., Giobbie-Hurder A., Felt K., Gjini E., Shukla S.A., Hu Z., Li L., Le P.M., Allesøe R.L., Richman A.R., Kowalczyk M.S., Abdelrahman S., Geduldig J.E., Charbonneau S., Pelton K., Iorgulescu J.B., Elagina L., Zhang W., Olive O., McCluskey C., Olsen L.R., Stevens J., Lane W.J., Salazar A.M., Daley H., Wen P.Y., Chiocca E.A., Harden M., Lennon N.J., Gabriel S., Getz G., Lander E.S., Regev A., Ritz J., Neuberg D., Rodig S.J., Ligon K.L., Suvà M.L., Wucherpfennig K.W., Hacohen N., Fritsch E.F., Livak K.J., Ott P.A., Wu C.J, & Reardon D.A. (2018). Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trial. Nature, 565(7738), 234-239.
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4

Multispectral Imaging for Tumor Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Image acquisition was performed using the Mantra multispectral imaging platform (Vectra 3, PerkinElmer, Hopkinton, MA) as previously described 34 (link). Areas with non-tumor or residual normal tissue (i.e. residual lymph node) were excluded from the analysis. Representative regions of interest were chosen by the pathologist, and 5–7 fields of view (FOVs) were acquired at 20× resolution as multispectral images. Image Analysis was performed using the Inform 2.3 Image Analysis Software (PerkinElmer, Hopkinton, MA).
Cañadas I., Thummalapalli R., Kim J.W., Kitajima S., Jenkins R.W., Christensen C.L., Campisi M., Kuang Y., Zhang Y., Gjini E., Zhang G., Tian T., Sen D.R., Miao D., Imamura Y., Thai T., Piel B., Terai H., Aref A.R., Hagan T., Koyama S., Watanabe M., Baba H., Adeni A.E., Lydon C.A., Tamayo P., Wei Z., Herlyn M., Barbie T.U., Uppaluri R., Sholl L.M., Sicinska E., Sands J., Rodig S., Wong K.K., Paweletz C.P., Watanabe H, & Barbie D.A. (2018). Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Nature medicine, 24(8), 1143-1150.
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