100 nm polycarbonate membrane

Manufactured by Cytiva

Sourced in United Kingdom

The 100 nm polycarbonate membranes are a type of lab equipment used for filtration and separation applications. These membranes have a pore size of 100 nanometers and are made of polycarbonate, a durable and chemically-resistant material. The membranes can be used to filter and separate particles, molecules, or cells based on their size.

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Lab products found in correlation

Mini extruder, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope, by Avanti Polar Lipids (2 mentions) Cholesterol, by Merck Group (2 mentions) Liposofast extruder device, by Merck Group (1 mentions) 35 mm glass bottom imaging dish, by MatTek (1 mentions) Nano zsp, by Malvern Panalytical (1 mentions) Dspe peg, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Anatrace (1 mentions) 1 2 dioleoyl sn glycero 3 phospho l serine dops, by Avanti Polar Lipids (1 mentions) Tris hcl, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions) Avanti mini extruder, by Avanti Polar Lipids (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Cymal 5, by Anatrace (1 mentions) Zeba spin desalting column, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

100 nm polycarbonate membrane filter 100 nm membrane Polycarbonate membranes

14 protocols using 100 nm polycarbonate membrane

Lipid-DNA Liposome Preparation

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An appropriate amount of freeze-dried lipid–DNA was mixed with DOPC : DOPE : Chol (50 : 25 : 25 mol% in chloroform) to reach a total of 0.1 mM lipid with 0.5 mol% lipid–DNA. Afterwards, chloroform was removed by evaporation under nitrogen and then under vacuum overnight. The dried lipid : lipid–DNA mixture was dissolved in an aqueous PBS buffer (150 mM NaCl, 15 mM K₂HPO₄, 5 mM KH₂PO₄) by 5 cycles of vortexing and freeze–thawing. Subsequently, the sample was dispersed by extruding 21 times, using an extruder and 100 nm polycarbonate membranes (Whatman), to obtain large unilamellar vesicles (LUVs), after which the liposomes with lipid–DNA were incubated at 50 °C for 1 h. All lipid–DNA liposomes were used within one day and had an average diameter of around 120 nm as determined by DLS (ALV/CGS-3 ALV-Laser Vertriebsgesellschaft m-b.H., Langen, Germany). The molar ratio between lipid and U4T18 was 500 : 1. The Final lipid concentration was 0.45 mM.

Yang J., Meng Z., Liu Q., Shimada Y., Olsthoorn R.C., Spaink H.P., Herrmann A, & Kros A. (2018). Performing DNA nanotechnology operations on a zebrafish †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01771a. Chemical Science, 9(36), 7271-7276.

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Preparation of POPC/POPS Liposomes

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POPC/POPS (7:3) liposomes at 10 mg/ml were prepared as follows: 7 mg POPC and 3 mg POPS were dissolved in chloroform followed by thorough evaporation of solvent. The lipid mixture was then resuspended in 1 ml HBSS, and suspensions were extruded using 100 nm polycarbonate membranes (Whatman, article no. 800309) and a LiposoFast extruder device (Sigma‐Aldrich) to generate large unilamellar vesicles.

Schlepckow K., Monroe K.M., Kleinberger G., Cantuti‐Castelvetri L., Parhizkar S., Xia D., Willem M., Werner G., Pettkus N., Brunner B., Sülzen A., Nuscher B., Hampel H., Xiang X., Feederle R., Tahirovic S., Park J.I., Prorok R., Mahon C., Liang C., Shi J., Kim D.J., Sabelström H., Huang F., Di Paolo G., Simons M., Lewcock J.W, & Haass C. (2020). Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region. EMBO Molecular Medicine, 12(4), e11227.

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Synthesis of Liposomal Photosensitizers

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For the synthesis of lipo-IRDye700DX, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (Avanti), cholesterol (Avanti), DSPE-mPEG₂₀₀₀ (Avanti), and DSPE-PEG-IRDye700DX were mixed in chloroform at a molar ratio of 0.67, 0.30, 0.025, and 0.005, respectively. For the synthesis of lipo-BPD-PC, the lipids DPPC, cholesterol, DSPE-mPEG₂₀₀₀, and 20:0 lyso-PC-BPD were mixed in chloroform at a molar ratio of 0.665, 0.30, 0.03, and 0.005, respectively. Liposomes were prepared using a conventional thin-film hydration-extrusion method, by hydration in PBS and extrusion through 100 nm polycarbonate membranes (Whatman) [18 (link)–21 (link), 39 (link)].

Guirguis M., Bhandari C., Li J., Eroy M., Prajapati S., Margolis R., Shrivastava N., Hoyt K., Hasan T, & Obaid G. (2021). Membrane composition is a functional determinant of NIR-activable liposomes in orthotopic head and neck cancer. Nanophotonics, 10(12), 3169-3185.

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Biotin-Labeled Lipid Membrane Formation

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A chloroform solution of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine with 0.1 mole percent 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (Avanti Polar Lipids) was dried first under a stream of nitrogen and then under vacuum to remove the solvent. The resulting film was dissolved to 1 mg/mL in PBS pH 7.4 at 37 °C with extensive vortexing and freeze-thawed 7x by cycling between liquid nitrogen and 37 °C baths. The lipid suspension was then extruded 13x through double stacked 100 nm polycarbonate membranes (Whatman) to yield small unilamellar vesicles (SUVs). SUVs prepared in this way were stored at 4 °C and used within 2 weeks. Silicon wafers with ~1900 nm thermal oxide (Addison Engineering) were diced into 1 cm² chips, and the oxide layer thickness for each chip was measured with a FilMetrics F50-EXR. The chips were then cleaned in piranha solution (30 volume % hydrogen peroxide in sulfuric acid), rinsed extensively and stored in deionized water until use. SLBs were formed by incubating the cleaned silicon chips in the SUV solution for 1 hour, and then rinsed with PBS, incubated with 1.75 μM DiIC₁₈ for 30 minutes, and rinsed again with PBS. The samples were then inverted into a 35 mm glass-bottom imaging dish (MatTek) containing PBS and immediately imaged.

Colville M.J., Park S., Zipfel W.R, & Paszek M.J. (2019). High-speed device synchronization in optical microscopy with an open-source hardware control platform. Scientific Reports, 9, 12188.

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Liposomal Benzoporphyrin Derivative for Photodynamic Therapy

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PEG-coated liposomal benzoporphyrin derivative (Lipo-BPD) was used for PDP in this study. Briefly, lipo-BPD was synthesized as per the conventional thin film hydration method. All lipids were purchased from Avanti Polar Lipids. Firstly, DPPC, cholesterol, DSPE-mPEG₂₀₀₀ and Benzoporphyrin Derivative Monoacid Ring A (US Pharmacopeia) were mixed together in chloroform at a molar ratio of 0.66:0.289:0.03:0.01 (27 (link)). 1X DPBS was used to hydrate the thin film at 42°C and extrusion was performed through 100 nm polycarbonate membranes (Whatman).

Bhandari C., Fakhry J., Eroy M., Song J.J., Samkoe K., Hasan T., Hoyt K, & Obaid G. (2022). Towards Photodynamic Image-Guided Surgery of Head and Neck Tumors: Photodynamic Priming Improves Delivery and Diagnostic Accuracy of Cetuximab-IRDye800CW. Frontiers in Oncology, 12, 853660.

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Preparation of 100 nm POPC:POPG LUVs

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Large unilamellar vesicles (LUVs) with a diameter of 100 nm were prepared as described previously.[27 (link)] Briefly, POPC and POPG lipids were first dissolved in chloroform and mixed at a 7:3 POPC:POPG molar ratio for mimicking a mixed anionic/zwitterionic membrane system. The solvent was removed under a steam of nitrogen gas and dried overnight to generate a lipid film which was then rehydrated in 20 mM HEPES (pH 7.4) containing 50 mM NaCl. Lipid suspension was subjected to 5 rounds of freeze-thaw and the resulting solution was extruded 23 times through 100-nm polycarbonate membranes (Whatman, Clifton, NJ) using a mini-extruder (Avanti Polar Lipids, Inc., Alabaster, AL). Unless otherwise noted, LUVs consisting of 250 μM lipids were used in this study.

Lee Y.H., Lin Y., Cox S.J., Kinoshita M., Sahoo B.R., Ivanova M, & Ramamoorthy A. (2018). Zinc Boosts EGCG’s hIAPP Amyloid Inhibition Both in Solution and Membrane. Biochimica et biophysica acta. Proteins and proteomics, 1867(5), 529-536.

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Synthesis of Liposomal Photosensitizers

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Gd-Loaded Liposomes Synthesis and Characterization

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Gadolinium diethylenetriaminepentaacetate (Gd; Sigma-Aldrich) was incorporated into 100-nm liposomes. A lipid mixture of hydrogenated soybean phosphatidylcholine (HSPC; Avanti) cholesterol (Sigma-Aldrich), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl (Rhod-DSPE; Avanti) and PEG-DSPE (Avanti) at a molar ratio of 55.94:39:0.06:5 respectively were dissolved in absolute ethanol at 70°C. The dissolved lipids were quickly injected into a PBS solution containing 270 mg/ml of Gd, at 70°C, to reach a lipid concentration of 50mM. The liposomes were extruded five times through 100-nm polycarbonate membranes (Whatman) at 70°C. The free Gd was removed by dialysis in a 12-14kDa membrane (Spectrum Laboratories) against PBS solution at 4°C for 24 hours. Liposomes were sized using a Zetasizer NanoZSP (Malvern).

Zinger A., Koren L., Adir O., Poley M., Alyan M., Yaari Z., Noor N., Krinsky N., Simon A., Gibori H., Krayem M., Mumblat Y., Kasten S., Ofir S., Fridman E., Milman N., Lübtow M.M., Liba L., Shklover J., Shainsky-Roitman J., Binenbaum Y., Hershkovitz D., Gil Z., Dvir T., Luxenhofer R., Satchi-Fainaro R, & Schroeder A. (2019). Collagenase Nanoparticles Enhance the Penetration of Drugs into Pancreatic Tumors. ACS nano, 13(10), 11008-11021.

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Preparation of Liposome Nanoparticles

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1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) (Avanti Polar Lipids), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Anatrace), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) (Avanti Polar Lipids) and cholesterol (Sigma) were dissolved in chloroform and mixed (3:2:3:2 w/w ratio). The lipid mix was dried under nitrogen gas and rehydrated in Buffer A. Liposomes were extruded through a 100 nm polycarbonate membrane (Whatman) to create a monodisperse unilamellar liposome population.

Boyd C.M., Parsons E.S., Smith R.A., Seddon J.M., Ces O, & Bubeck D. (2016). Disentangling the roles of cholesterol and CD59 in intermedilysin pore formation. Scientific Reports, 6, 38446.

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Preparation of Fluorescent Lipid Bilayer Vesicles

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A lipid film composed of 1,2‐dioleoyl‐sn‐glycero‐3‐phosphocholine (DOPC) (Avanti Polar Lipids), 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine (DOPE) (Avanti Polar Lipids) and cholesterol (Sigma Aldrich) with a mass ratio of 50:25:25 (total mass 1.25 mg) and 2 mol% additional 1,1′‐Dioctadecyl‐3,3,3′,3′‐Tetramethylindodicarbocyanine Perchlorate (DiD’) (Invitrogen, Thermo Fisher Scientific) was prepared via evaporation from a chloroform solution in a glass vial. After being thoroughly dried by lyophilisation (Labconco), it was rehydrated (1.25 mg/ml) in TE buffer (pH 8.0) containing 10 mM Tris‐HCl (Sigma‐Aldrich), 1 mM EDTA (Sigma‐Aldrich) and 150 mM NaCl (Sigma‐Aldrich) at 37 ˚C for 1 h. After being fully resuspended by vortex, the emulsion was extruded 31 times through a 100 nm polycarbonate membrane (Whatman) at 37 ˚C using an Avanti MiniExtruder (Avanti Polar Lipids). The liposome suspension was stored in a low adsorption glass vial (Supelco, Sigma‐Aldrich) at 4 ˚C protected from light, until use.

Görgens A., Corso G., Hagey D.W., Jawad Wiklander R., Gustafsson M.O., Felldin U., Lee Y., Bostancioglu R.B., Sork H., Liang X., Zheng W., Mohammad D.K., van de Wakker S.I., Vader P., Zickler A.M., Mamand D.R., Ma L., Holme M.N., Stevens M.M., Wiklander O.P, & EL Andaloussi S. (2022). Identification of storage conditions stabilizing extracellular vesicles preparations. Journal of Extracellular Vesicles, 11(6), e12238.

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