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Diammonium hydrogen phosphate

Manufactured by Merck Group
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Diammonium hydrogen phosphate is a chemical compound with the formula (NH4)2HPO4. It is a white crystalline solid that is soluble in water. Diammonium hydrogen phosphate is commonly used as a laboratory reagent and in various industrial applications.

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Lab products found in correlation

Calcium nitrate tetrahydrate, by Merck Group (17 mentions) Ammonium hydroxide, by Merck Group (9 mentions) Sodium hydroxide (naoh), by Merck Group (7 mentions) Hydrochloric acid (hcl), by Merck Group (5 mentions) Cetyltrimethylammonium bromide, by Merck Group (4 mentions) Ethanol, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Methanol, by Merck Group (4 mentions) Chitosan, by Merck Group (3 mentions) Strontium nitrate, by Merck Group (3 mentions) Calcium chloride, by Merck Group (3 mentions) Acetic acid, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Phosphoric acid, by Merck Group (3 mentions) Calcium chloride dihydrate, by Merck Group (2 mentions) Ammonia solution, by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Polyvinyl alcohol (pva), by Merck Group (2 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (2 mentions) Potassium dihydrogen phosphate, by Merck Group (2 mentions)

Close spelling variants of this product

Diammonium hydrogen phosphate ((NH4)2HPO4) (12 citations) Diammonium phosphate (5 citations)

37 protocols using diammonium hydrogen phosphate

1

Synthesis of Iron Oxide Nanoparticles

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Ferric chloride hexahydrate (FeCl3·6H2O), ferrous chloride tetrahydrate (FeCl2·4H2O), Calcium chloride dihydrate (CaCl2.2H2O), diammonium hydrogen phosphate ((NH4)2(HPO4)), Cetyl trimethylammonium bromide (CTAB), ammonium hydroxide (NH4OH) and the other regents all were purchased locally from Sigma-Aldrich Chemicals and used as received.
Kermanian M., Naghibi M, & Sadighian S. (2020). One-pot hydrothermal synthesis of a magnetic hydroxyapatite nanocomposite for MR imaging and pH-Sensitive drug delivery applications. Heliyon, 6(9), e04928.
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2

Synthesis of nHA and GNS Coatings on Ti6Al7Nb

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Ti6Al7Nb alloy was used as the substrate for the synthesis of nHA and GNS containing coatings in this study. The chemical composition of the substrate is presented in Table 1. The alloy plates supplied with 50 × 50 × 0.4 cm and then, cut by using a water jet machine with dimensions of 2.5 × 2.5 cm2. The cut surfaces were ground with sandpapers step by step from 240 to 1200 to remove the cutting traces formed on their surfaces. Then, the surfaces were ultrasonically cleaned with acetone, ethanol and deionized water, respectively.

Chemical composition of Ti6Al7Nb alloy (in wt.%)

AlNbFeONTi
6.127.070.120.180.01bal.
Disodium edetate (C10H14N2Na2O8·2H2O), sulfuric acid (H2SO4, 98%), hydrogen peroxide (H2O2, 30% aq.), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), di-ammonium hydrogen phosphate ((NH4)2HPO4) and ammonium hydroxide (NH4OH 28% aq.) were purchased commercially from Sigma-Aldrich. Graphene nanosheets (GNS) were purchased from Nanografi Company with 99% pureness. All chemicals were used analytically and without further purification.
Yigit O., Dikici B, & Ozdemir N. (2021). Hydrothermal synthesis of nanocrystalline hydroxyapatite–graphene nanosheet on Ti-6Al-7Nb: mechanical and in vitro corrosion performance. Journal of Materials Science. Materials in Medicine, 32(4), 40.
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3

Hydroxyapatite Materials Synthesis and Characterization

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All the chemicals were used as received from the manufacturer. For the preparation of the HA materials, calcium nitrate tetrahydrate (Ca(NO3)·4H2O, AR), triethyl phosphate (P(OC2H5)3, AR), diammonium hydrogen phosphate ((NH4)2HPO4, AR), Pluronic F127, polyvinyl alcohol (PVA), and 10× concentrated phosphate buffered saline (10× PBS) all came from Sigma-Aldrich, while anhydrous ethanol was from Merck (Darmstadt, Germany). Protein adsorption and cell culture studies required bovine serum albumin (BSA), vitronectin, BCATM Protein Assay Kit, hexamethyldisilane (HMDS), and sodium dodecyl sulphate (SDS, AR) all from Sigma-Aldrich; Minimum Essential Media (MEM), Glutamax, Non-Essential Amino Acids (NEAA), Antibiotic-Antimycotic solution (Anti-Anti), and TrypLE Express were purchased from Invitrogen; MTS reagent from Promega, and Fetal Bovine Serum (FBS) from SAFC Biosciences (Hampshire, England). The water used in all experiments was passed through a Millipore (Boston, MA, USA) Milli Q ultrapure water purification system and had a resistivity higher than 18.2 MΩcm.
Zhang X., Zhang L., Li Y., Hua Y., Li Y., Li W, & Li W. (2019). Template-assisted, Sol-gel Fabrication of Biocompatible, Hierarchically Porous Hydroxyapatite Scaffolds. Materials, 12(8), 1274.
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4

Synthesis of Nanostructured Hydroxyapatite

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PCL with molecular weight (M.W.) 80,000 g·mol−1, MET, dichloromethane (DCM), dimethylformamide (DMF), poly(vinyl alcohol) (PVA), diammonium hydrogen phosphate ((NH4)2HPO4), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O) and ammonium hydroxide solution (NH4OH) were acquired from Sigma-Aldrich GmbH, Steinheim, Germany. Darvan 821A was purchased from R. T. Vanderbilt, Norwalk, CT, USA. Darvan 821A is an ammonium polyacrylate mainly used as a dispersing agent to avoid the formation of particle aggregates of nHAP in homogeneous and highly concentrated nHAP colloidal suspension. Deionized water was used in all experiments. The resistivity of absolute pure water was 18.2 MΩ·cm at 25 °C or electrical conductivity 0.055 µS/cm. All commercial materials were used without further purification.
Mirică I.C., Furtos G., Lucaciu O., Pascuta P., Vlassa M., Moldovan M, & Campian R.S. (2021). Electrospun Membranes Based on Polycaprolactone, Nano-Hydroxyapatite and Metronidazole. Materials, 14(4), 931.
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5

Bioactive Scaffold for Bone Regeneration

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The fruits of EA were obtained from South Khorasan, Birjand, Iran. Calcium nitrate tetrahydrate (Ca (NO3)2·4H2O), diammonium hydrogen phosphate ((NH4)2HPO4), PCL, cetylpyridinium chloride, alizarin red S, penicillin–streptomycin, MTT, Triton X-100, ascorbic acid, dexamethasone, and β-glycerol phosphate disodium salt pentahydrate were supplied from Sigma-Aldrich, St. Louis, MO. Dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH) and acetic acid provided from Merck Chemical Co. Fetal bovine serum (FBS) was supplied from Capricorn, South America. Dulbecco’s modified eagle medium (DMEM) was supplied Gibco, Grand Island.
Azaryan E., Hanafi-Bojd M.Y., Alemzadeh E., Emadian Razavi F, & Naseri M. (2022). Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells. BMC Oral Health, 22, 505.
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6

Synthesis of Bismuth Phosphate

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Bismuth nitrate pentahydrate [Bi(NO3)3·5H2O, 99.99%], diammonium hydrogen phosphate [(NH4)2HPO4 > 99.0%], dimethylsulfoxide (DMSO > 99.9%) and cetyltrimethylammonium bromide (CTAB, 98%) were delivered from Sigma-Aldrich.
Azzam A.B., El-Sheikh S.M., Geioushy R.A., Salah B.A., El-Dars F.M, & Helal A.S. (2019). Facile fabrication of a novel BiPO4 phase junction with enhanced photocatalytic performance towards aniline blue degradation. RSC Advances, 9(30), 17246-17253.
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7

Synthesis and Characterization of Nanocomposites

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6-Gingerol (> 98.0%, HPLC), Doxorubicin hydrochloride (98.0–102.0%, HPLC), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O, 99%, ACS), diammonium hydrogen phosphate ((NH4)2HPO4, > 99.0%), ammonium iron(II) sulfate hexahydrate ((NH4)2Fe(SO4)2·6H2O, 99.0%, ACS), ammonium iron(III) sulfate dodecahydrate (NH4Fe(SO4)2·12H2O, 99.0%, ACS), ethanol (EtOH, > 99.8%, HPLC), methanol anhydrous (MeOH, 99.8%), alginic acid sodium salt (NaAlg, low viscosity), Cetyltrimethyl ammonium bromide (CTAB, > 98%) and TWEEN®80 (Viscous liquid), and ammonium hydroxide solution (puriss. p.a., 25% NH3 in H2O) were purchased from Sigma Aldrich, Bangalore, India. Polyethylene glycol 200 (PEG 200) was purchased from Merck Millipore Corporation, Darmstadt, Germany. Snakeskin dialysis tubing (MWCO 3.5 kDa) was purchased Thermo Fisher, Bangalore, India.
Manatunga D.C., de Silva R.M., de Silva K.M., Wijeratne D.T., Malavige G.N, & Williams G. (2018). Fabrication of 6-gingerol, doxorubicin and alginate hydroxyapatite into a bio-compatible formulation: enhanced anti-proliferative effect on breast and liver cancer cells. Chemistry Central Journal, 12, 119.
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8

Synthesis of PCL-based Biomaterials

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Poly(ε-caprolactone) (PCL, Mn:5000, CELLINK, Gothenburg, Sweden), sodium hydroxide (NaOH, Sigma-Aldrich, St. Louis, MO, USA), ethanol (99.5%, Sigma-Aldrich) were purchased and used as received without any further purification. Distilled-deionized (DI) water from a Millipore Milli-Q ultrapure water system was used in the experiments. Magnesium nitrate hexahydrate and diammonium hydrogen phosphate were procured from Sigma-Aldrich.
Dubey N., Ferreira J.A., Daghrery A., Aytac Z., Malda J., Bhaduri S.B, & Bottino M.C. (2020). Highly Tunable Bioactive Fiber-Reinforced Hydrogel for Guided Bone Regeneration. Acta biomaterialia, 113, 164-176.
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9

Synthesis of Biomineralization Compounds

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Calcium nitrate tetrahydrate (Ca(NO3)2.4H2O, 98.5%), diammonium hydrogen phosphate ((NH4)2HPO4, 99.5%, sodium selenite ((Na2SeO3), 99%), strontium nitrate (Sr(NO3)2, 99.99%), and ammonium hydroxide ((NH4OH), 28–30%) were analytical grade chemicals purchased from Sigma Aldrich (Dorset, UK).
Maqbool M., Nawaz Q., Atiq Ur Rehman M., Cresswell M., Jackson P., Hurle K., Detsch R., Goldmann W.H., Shah A.T, & Boccaccini A.R. (2021). Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite. International Journal of Molecular Sciences, 22(8), 4246.
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10

Polycyclic Aromatic Hydrocarbon Analysis

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In this study, benzo[a]pyrene (99.2%), phenanthrene (99.8%), naphthalene (99.9%), fluoranthene (99.3%), benzo[a]anthracene (99.2%), calcium chloride (CaCl2) (98%), diammonium hydrogen phosphate ((NH4)2HPO4) (99%), potassium dihydrogen phosphate (KH2PO4) (98%), ammonium peroxydisulfate ((NH4)2S2O8) (99%), acetic acid (99.8%), hydrochloric acid (98%), sodium hydroxide (98%), and aniline (99%) were obtained from Sigma-Aldrich and used without any purification. Also, high purity nitrogen (99.99%) was obtained from Raham Company (Tehran, Iran).
Alinaghi Langari A.A., Alizadeh S., Soury S., Firoozichahak A., Nematollahi D., Alizadeh P.M, & Sanaei N. (2020). Nano-hydroxyapatite/polyaniline composite as an efficient sorbent for sensitive determination of the polycyclic aromatic hydrocarbons in air by a needle trap device. RSC Advances, 10(69), 42267-42276.
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