P38 mapk rabbit mab

WT C57BL/6 mice aged from 6 to 8 weeks were obtained from the Experimental Animal Center of Chongqing Medical University. The lentiviral pLVX IRES Puro constructs to express GFP and PNPLA7-GFP Mission^® lentiviral pLKO.1 vectors encoding for scrambled shRNA or shRNAs targeting murine PNPLA7 were maintained in our lab [18 (link)]. LPS from Escherichia coli O111:B4 was obtained from Sigma. The MiniBEST Universal RNA Extraction Kit, PrimeScript™ II 1st Strand cDNA Synthesis Kit, and TB Green^® Fast qPCR Mix were purchased from TaKaRa. A PNPLA7 rabbit antiserum was generated in our lab by immunizing rabbits with a peptide in the regulatory domain of mouse PNPLA7. The generation of this antiserum and its specificity have been previously described [17 (link)]. The SIRT1 mouse monoclonal antibody (mAb) was purchased from Santa Cruz Biotechnology and β-actin mouse mAb, α-Tubulin mouse mAb, GAPDH mouse mAb, NF-κB p65 rabbit mAb, NF-κB1 p105/p50 rabbit mAb, iNOS antibody (Mouse Specific), IκBα (44D4) Rabbit mAb, p38 MAPK rabbit mAb, and Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb were obtained from Cell Signaling Technology. Anti-SOCS1 antibody and Anti-NF-κB p65 (acetyl K310) antibody were obtained from Abcam. Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor^® 647 conjugate was purchased from Thermo Fisher Scientific.

The following antibodies were used for western blot analysis: Rabbit anti-rat ANX A1 (ab65844) was obtained from Abcam (Cambridge, USA); and phospho-p44/42 MAPK (Thr202/Tyr204) rabbit mAb (catalogue no. 4370); p44/42 MAPK rabbit mAb (catalogue no. 4695); phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (catalogue no. 4511); p38 MAPK rabbit mAb (catalogue no. 8690); and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) rabbit mAb (catalogue no. 5174) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). All the primary antibodies were used at a dilution of 1:1,000. The secondary anti-rabbit IRDye 800CW antibody was obtained from LI-COR Biosciences (Lincoln, NE, USA).

Dihydroartemisinin was obtained from Ourchem (Sinopharm Chemical Reagent Co, Ltd, China). Dimethyl sulfoxide (DMSO), cisplatin, acridine orange (AO), deferoxamine (DFO), N-acetyl-L-cysteine (NAC), and ferric ammonium citrate (FAC) were purchased from Sigma (USA). CCK-8 kit was obtained from Dojindo (Japan). EdU Imaging Kit was bought from Ribobio (China). Lysosome Staining Kit was obtained from Abnova (USA). Lysotracker probe and Mito-tracker Red probe were procured from Beyotime (China). ROS Kit and JC-1 kit were acquired from Beyotime (China). AO/EB Staining Kit was obtained from Senbeijia (China). Primary antibodies for rabbit polyclonal to LC3B (Abcam, ab48394), rabbit monoclonal to Cathepsin B (Abcam,ab125067), mouse monoclonal to GAPDH (Abcam, ab8245), SQSTM1/p62 mouse mAb(Cell Signaling Technology,#8588), ATG5 rabbit mAb (Cell Signaling Technology,#12994), ATG12 rabbit mAb (Cell Signaling Technology,#4180), ATG14 rabbit mAb (Cell Signaling Technology,#96752), Erk1/2 rabbit mAb(Cell Signaling Technology,#4695), p-Erk1/2 rabbit mAb (Cell Signaling Technology,#8544), p38 MAPK rabbit mAb (Cell Signaling Technology,#14451), p-p38 rabbit mAb (Cell Signaling Technology,#4631), SAPK/JNK rabbit mAb (Cell Signaling Technology,#9252), Phospho-SAPK/JNK Rabbit mAbp-JNK (Cell Signaling Technology,#4668), were used according to the protocol.