Uas 5ht1brnai

All flies were reared on standard cornmeal food at 25°C and 60% relative humidity on a 12 h:12 h light-dark cycle. R13F02-GAL4, VT30604-GAL4, VT0765-GAL4, VT41043-GAL4, VT64246-GAL4, APL-GAL4, L0111-LexA, tub-GAL80^ts, UAS-shi^ts, and UAS-mCD8:GFP;UAS-mCD8:GFP fly strains have been used in our previous studies (Wu et al., 2013 (link); Shih et al., 2015 (link); Wu et al., 2015 (link); Yang et al., 2016 (link); Shyu et al., 2017 (link); Wu et al., 2018 (link); Shyu et al., 2019 (link); Lee et al., 2020 (link)). The lexAop-spGFP₁₁, UAS-spGFP_1-10, lexAop-Brp::mCherry fly strains were obtained from Tzu-Yang Lin. The UAS-DDC^RNAi and UAS-Trh^RNAi fly strains were obtained from Ann-Shyn Chiang (Lee et al., 2011 (link)). The UAS-GAD^RNAi (v32344) fly strain was obtained from Vienna Drosophila RNAi Center. MB057B-GAL4, MB083C-GAL4, MB011B-GAL4, MB027B-GAL4, MB543B-GAL4, MB051B-GAL4, MB091C-GAL4, UAS-5HT1A^RNAi (TRiP.JF01852), UAS-5HT1B^RNAi (TRiP.JF01851), UAS-5HT2A^RNAi (TRiP.JF02157), UAS-5HT2B^RNAi (TRiP.HMJ22882), UAS-5HT7^RNAi (TRiP.JF02576), UAS-ChAT^RNAi (TRiP.JF01877), and UAS-VAChT^RNAi(TRiP.JF02764) fly strains were obtained from the Bloomington Drosophila Stock Center.

P. rapae larvae were collected primarily from cabbage fields in the experimental farmland of Zhejiang University, Hangzhou, China and the laboratory colony of P. rapae was reared at 25 ± 1°C and 70% relative humidity under a photoperiod of 14 hr light: 10 hr dark in a greenhouse as previously described by Zhang et al. (2005) (link).
The following fly stocks were from the Bloomington Drosophila Stock Center: HmlΔ-Gal4 (30139); UAS-5-HT_1B RNAi (27634, 25833); UAS-5-HT_2B RNAi (25874, 60488). The 5-HT_1B control and 5-HT_1B^ΔIII-Vflies were generous gifts from Leslie Vosshall (Gasque et al., 2013 (link)).

Drosophila melanogaster flies were raised on standard medium (water, cornmeal, soy bean, agar, molasses, yeast, preservative methyl-4-hydroxybenzoate) at 25 °C, 60% humidity and 14 h/10 h light–dark cycles. Canton-S (CS) served as wild-type Drosophila strain in this study. The following colleagues kindly provided additional stocks: D. Armstrong (189Y-GAL4), M. Heisenberg (wild-type Canton-S, rut¹, and dnc¹), T.J. Hirsh (Trh493-GAL4), Y. Rao (5HT-1A-GAL4), H. Scholz (UAS-TNT), P. Shen (UAS-npf), H. Tanimoto (H24-GAL4, NP1131-GAL4 and mb247-GAL4; Tub-GAL80^ts), and T. Zars (mb247-GAL4). The following lines were obtained from the Bloomington Stock Center: Or83b-GAL4 (#2681), c305a-GAL4 (#30829), c739-GAL4(#7362), npf-GAL4 (#25681), 5HT-1B-GAL4 (#27637), UAS-NaChBac (#9469), UAS-5HT-1A^RNAi (#25834 and #33885), UAS-5HT-1B^RNAi (#27634 and #54006), UAS-CD4::tdTomato (#35837) and UAS-mCD8::GFP (#5130).