Anti mouse cd326 epcam pe cy7

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-mouse CD326 (EpCAM) PE-Cy7 is a fluorescently-labeled antibody that recognizes the mouse CD326 (also known as EpCAM) antigen. It is designed for use in flow cytometry applications to identify and characterize cells expressing the CD326 antigen.

Automatically generated - may contain errors

Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Anti mouse cd45 fitc, by BD (1 mentions) Facsaria flow cytometer, by BD (1 mentions) Myeloperoxidase from human leukocytes, by Merck Group (1 mentions) Fitc phalloidin, by Merck Group (1 mentions) Duoset enzyme linked immunosorbent assay elisa kit, by R&D Systems (1 mentions) Anti mouse cd11c apc, by Thermo Fisher Scientific (1 mentions) 6 shogaol, by Merck Group (1 mentions) 6 gingerol, by Merck Group (1 mentions) Rabbit anti mouse e cadherin antibody, by Santa Cruz Biotechnology (1 mentions) O dianisidine dihydrochloride, by Merck Group (1 mentions) Collagenase type 8, by Merck Group (1 mentions) Dnase 1, by Merck Group (1 mentions)

2 protocols using anti mouse cd326 epcam pe cy7

Lung Cell Separation and RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Left lung from mice were harvested 72 h after RSV infection and acetate treatment. The lung was immediately submitted to collagenase IV (1 mg/ml) digestion protocol and total cells were stained with anti-mouse CD45 FITC (1:200, #553080, clone 30-F11) (BD Biosciences®) and anti-mouse CD326 (EpCAM) PE-Cy7 (1:100, #25–5791–80, clone G8.8) (eBioscience) antibodies for 30 min. Cells were acquired on BD FACSAria flow cytometer (BD Biosciences®) and sorted in two different populations: CD45⁺CD326⁻ (leukocytes/lymphoid cells) and CD45⁻CD326⁺ (epithelial cells). Right after the separation of the populations, cells were centrifuged, suspended in TRIzol (Thermo Fisher Scientific) and processed for RNA extraction and cDNA synthesis. The Ifnb1 expression was made as described below.

Antunes K.H., Fachi J.L., de Paula R., da Silva E.F., Pral L.P., dos Santos A.Á., Dias G.B., Vargas J.E., Puga R., Mayer F.Q., Maito F., Zárate-Bladés C.R., Ajami N.J., Sant’Ana M.R., Candreva T., Rodrigues H.G., Schmiele M., Silva Clerici M.T., Proença-Modena J.L., Vieira A.T., Mackay C.R., Mansur D., Caballero M.T., Marzec J., Li J., Wang X., Bell D., Polack F.P., Kleeberger S.R., Stein R.T., Vinolo M.A, & de Souza A.P. (2019). Microbiota-derived acetate protects against respiratory syncytial virus infection through a GPR43-type 1 interferon response. Nature Communications, 10, 3273.

+ Open protocol

+ Expand

Fluorescent Lipophilic Dyes and Immune Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescent lipophilic dyes, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiL), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1′-dioctadecyl-3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), were purchased from Promokine (Heidelberg, Germany); DC-Chol/DOPE Blend was from Avanti Polar Lipids (Alabaster, AL, USA); phalloidin-FITC, O-dianisidine dihydrochloride, myeloperoxidase from human leukocytes, type VIII collagenase, DNase I, (6)-gingerol and (6)-shogaol standards were purchased from Sigma (St. Louis, MO, USA). Rabbit anti-mouse E-cadherin antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse CD326 (EpCAM) PE-Cy7, anti-mouse CD11b eFluo 450; anti-mouse CD11c APC, and anti-mouse F4/80 antigen PE-Cy7 were purchased from eBioscience (San Diego, CA, USA). Duoset enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN, USA).

Zhang M., Viennois E., Prasad M., Zhang Y., Wang L., Zhang Z., Han M.K., Xiao B., Xu C., Srinivasan S, & Merlin D. (2016). Edible Ginger-Derived Nanoparticles: A Novel Therapeutic Approach for the Prevention and Treatment of Inflammatory Bowel Disease and Colitis-Associated Cancer. Biomaterials, 101, 321-340.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!