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Neutravidin coated microplates

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NeutrAvidin-coated microplates are multi-well plates designed for high-throughput applications. The plates are coated with the NeutrAvidin protein, which binds to biotinylated molecules. This allows for the immobilization and capture of biotin-labeled targets, such as proteins, nucleic acids, or cells, within the microplate wells.

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Lab products found in correlation

Duosets, by R&D Systems (2 mentions) Biotin conjugated ccp peptides, by Thermo Fisher Scientific (2 mentions) Bicinchoninic acid protein assay reagent, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Mouse ana elisa kit, by Alpha Diagnostic (2 mentions)

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NeutrAvidin

2 protocols using neutravidin coated microplates

1

Quantifying Immune Markers in Arthritis

Cited 1 time
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Inflammatory cytokines, chemokines and growth factors in ankles or sera were determined using ELISAs specific for each molecule (DuoSets, R&D). Ankles were homogenized in PBS supplemented with a protease inhibitor cocktail (Sigma). Supernatants were collected using centrifugation at 12,000g for 5 min at 4 °C, and the protein concentration was determined using bicinchoninic acid protein assay reagents (Thermo Scientific)58 (link)60 (link). Immunoglobulins were quantified using ELISA (Southern Biotechnology Association). Antinuclear antibodies (ANA) were determined utilizing a mouse ANA ELISA kit (Alpha Diagnostic International). RF in serum diluted 1:25 was detected employing 1 μg ml−1 rabbit IgG (Dako, X0903)-coated ELISA plates61 (link). ELISA for antiCCP (anti-CCP) antibodies was performed as described with modification62 (link). Briefly, biotin-conjugated CCP peptides were synthesized (ThermoFisher) and bounded (1 μg ml−1) to NeutrAvidin-coated microplates (ThermoFisher). Mouse sera (1:100) were incubated for 1 h at room temperature followed by incubation with HRP-conjugated sheep anti-mouse IgG. The results are presented as the product of OD450 nm × serum dilution. Previously described methods were employed to generate synovial proteome microarrays, which were probed with mouse serum63 (link).
Huang Q.Q., Perlman H., Birkett R., Doyle R., Fang D., Haines G.K., Robinson W., Datta S., Huang Z., Li Q.Z., Phee H, & Pope R.M. (2015). CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nature Communications, 6, 7086.
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2

Quantification of Inflammatory Markers in Murine Arthritis

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Inflammatory cytokines, chemokines and growth factors in ankles or sera were determined by ELISAs specific for each molecule (DuoSets, R&D). Ankles were homogenized in PBS supplemented with a protease inhibitor cocktail (Sigma). Supernatants were collected by centrifugation at 12,000g for 5 minutes at 4°C, and the protein concentration was determined using bicinchoninic acid protein assay reagents (Thermo Scientific)58 , 60 (link). Immunoglobulins were quantified by ELISA (Southern Biotechnology Association). Antinuclear antibodies (ANA) were determined utilizing a mouse ANA ELISA kit (Alpha Diagnostic International). Rheumatoid factor (RF) in serum diluted 1:25 was detected employing 1 μg ml−1 rabbit IgG (Dako, X0903) coated ELISA plates61 (link). ELISA for anti-cyclic citrullinated peptide (anti-CCP) antibodies was performed as described with modification62 (link). Briefly, biotin-conjugated CCP peptides were synthesized (ThermoFisher) and bounded (1 μg ml−1) to NeutrAvidin coated microplates (ThermoFisher). Mouse sera (1:100) were incubated for 1 hour at room temperature followed by incubation with HRP-conjugated sheep anti-mouse IgG. The results are presented as the product of OD450nm x serum dilution. Previously described methods were employed to generate synovial proteome microarrays, which were probed with mouse serum63 (link).
Huang Q.Q., Perlman H., Birkett R., Doyle R., Fang D., Haines G.K., Robinson W., Datta S., Huang Z., Li Q.Z., Phee H, & Pope R.M. (2015). CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nature Communications, 6, 7086.
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