Nrf2 (Cat.66504), Lamin B (Cat.66095) and β‐actin (Cat.60008) antibodies were purchased from Proteintech (Boston, MA, USA). PPARα (ab8934) and PPARγ (ab191407) antibodies were provided by Abcam (Cambridge, MA, USA). Antibodies against HO‐1 (D60G11) (5853S), AMPKα (5831T), p‐AMPKα (2535T), AMPKβ (4150T), p‐AMPKβ (4186T), adenosine ACC (3676T), p‐ACC (11818T) were purchased from Cell Signal Technology (Boston, MA, USA). Protein kinase B (AKT) (AF6261), p‐AKT (Thr 308) (AF3262), GAPDH (AF7021) were purchased from Affinity (OH, USA). HRP‐conjugated goat anti‐rabbit and goat anti‐mouse antibodies were provided by Boster (California, USA). FG super sensitive ECL luminescence reagent was provided by Meilunbio (Dalian, China).
Pparα ab8934
Manufactured by Abcam
Sourced in United States, Hong Kong
PPARα (ab8934) is a protein that functions as a nuclear receptor and transcription factor. It plays a role in the regulation of genes involved in lipid and glucose metabolism. This product is intended for research use only.
Automatically generated - may contain errors
2 protocols using pparα ab8934
1
Molecular Pathway Regulation Analysis
2
Neurotensin Receptor Regulation in PPAR
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NTR1 antibody (Cat. No. sc-374492) was from Santa Cruz Biotechnology (Dallas, TX). Anti-PPARα (phospho S12) antibody (Cat. No. ab3484), PPARα (ab8934) and β-Actin (ab8226) antibodies were obtained from Abcam (NT, HK). NTR3 (Cat. No. 12369-1-AP) and PPARα (Cat. No. 15540-1-AP) antibodies were obtained from Proteintech (Rosemont, IL). Neurotensin (Cat. No. N5266), SR 48692 (Cat. No. SML0278), Sodium taurocholate-2, 2, 4, 4-d4 (TCA-D4, Cat. No. 900036) and Anti-ASBT antibody (Cat. No. HPA004795) were from Sigma-Aldrich (St. Louis, MO). SYBR-Green Universal Master Mix kit (Cat. No. 4385610) and a High Capacity cDNA Reverse Transcription kit (Cat. No. 4368814) were from Applied Biosystems (Foster City, CA). Dual Luciferase Assay Kit (Cat. No. E2920) was from Promega (Madison, WI). Lipofectamine® RNAiMAX (Cat. No. 11668019) and Lipofectamine™ 2000 (Cat. No. 11668027) transfection reagents and TRIzol reagent (Cat. No. 15596026) were from Life Technologies (Foster, CA). The siRNAs were synthesized by GenePharma (Shanghai, China). The sequences for siRNAs were described as previously and listed in Supplementary Materials and Methods.
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