Kidney sections from mice were prepared accordingly. Immunostaining was performed using anti–WT-1 antibodies (Santa Cruz Biotechnology, sc-192). After washing, sections were incubated with a fluorophore-linked secondary antibody (Invitrogen, Alexa Fluor 488 anti–rabbit IgG [A32731] and Alexa Fluor 568 anti–mouse IgG [A-11004]). After staining, slides were mounted in Aqua Poly Mount (Polysciences Inc., 18606-5) and imaged with a Zeiss AXIO Imager microscope with a digital camera.
Anti wt 1 antibodies
Manufactured by Santa Cruz Biotechnology
Anti-WT-1 antibodies are laboratory reagents used for the detection and identification of the Wilms' Tumor 1 (WT-1) protein. WT-1 is a transcription factor involved in the regulation of gene expression during development and can be used as a marker for certain types of cancer. These antibodies provide a tool for researchers to study the role of WT-1 in biological processes and disease states.
Automatically generated - may contain errors
Lab products found in correlation
Axioimager microscope, by Zeiss (1 mentions)
Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Aqua poly mount, by Polysciences (1 mentions)
Anti chop, by Cell Signaling Technology (1 mentions)
Anti ire1α, by Cell Signaling Technology (1 mentions)
Anti iκb, by Cell Signaling Technology (1 mentions)
Anti p iκb, by Cell Signaling Technology (1 mentions)
Anti caspase 12, by Cell Signaling Technology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti nlrp3, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 1, by Cell Signaling Technology (1 mentions)
Anti xbp1s antibody, by BioLegend (1 mentions)
Hrp conjugated anti mouse β actin antibody, by Merck Group (1 mentions)
Anti cytochrome c, by Cell Signaling Technology (1 mentions)
Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti nf κb, by Cell Signaling Technology (1 mentions)
Anti cox 4, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti il 1β, by Cell Signaling Technology (1 mentions)
Anti caspase 9, by Cell Signaling Technology (1 mentions)
2 protocols using anti wt 1 antibodies
1
Immunostaining of Murine Kidney Sections
2
Comprehensive Antibody Characterization Protocol
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Commercially available antibodies were obtained as follows: anti-caspase 12, anti-caspase 3, anti-caspase 9, anti-cleaved caspase 1, anti-p-JNK, anti-JNK, anti-p-eIF2α, anti-eIF2α, anti-IRE1α, anti-CHOP, anti-NLRP3, anti-COX IV, anti-IL1β, anti-cleaved caspase1, anti-p-IκB, anti-IκB, anti-NFκB, and anti-cytochrome c antibodies were from Cell Signaling Technology; anti-ATF6 and anti-p-IRE1α antibodies were from Novus Biological; anti-p-ASK1(Ser967) antibody was from Sigma-Aldrich; anti-XBP1s antibody was from BioLegend; anti-TXNIP antibody was from MBL International Corporation; anti-WT-1 antibodies were from Santa Cruz Biotechnology or Abcam; and anti-ATF4 and anti-Trx2 antibodies were from Santa Cruz Biotechnology. HRP-conjugated anti-mouse β-actin antibody was from Sigma-Aldrich and HRP-conjugated secondary antibodies were from Cell Signaling Technology.
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