3 t trim trio scanner

MRI data were collected at the Berlin Center for Advanced Neuroimaging using a 3 T Trim Trio scanner equipped with a 20-channel head coil (Siemens, Erlangen, Germany). Resting-state functional images were acquired using an echo planar imaging sequence (recognition time = 2.25 s, echo time = 30 ms, 260 volumes, voxel size = 3.4 mm × 3.4 mm × 3.4 mm), with a duration of 9:51 min. High-resolution T1-weighted structural scans were collected using a magnetization-prepared rapid gradient echo sequence (voxel size = 1 mm × 1 mm × 1mm). Lesion volume for MS patients was calculated based on a fluid-attenuated inversion recovery sequence (recognition time = 6000 ms, echo time = 388 ms, inversion time (TI) = 2100 ms, voxel size = 1 mm × 1 mm × 1 mm, matrix = 256 × 256, field of view (FOV) = 256 mm and 176 contiguous sagittal slices; see Supplementary Material 1 for a detailed description of lesion segmentation). Healthy controls with white or grey matter lesions were not included in this study.

Structural and functional MRI data were acquired at the Berlin Center for Advanced Neuroimaging at Charité-Universitätsmedizin Berlin using a 20-channel head coil and a 3 T Trim Trio scanner (Siemens, Erlangen, Germany). For resting-state functional MRI (rs-fMRI), we employed an echoplanar imaging sequence [repetition time (TR) = 2.25 s, echo time = 30 ms, 260 volumes, voxel size = 3.4 mm × 3.4 mm × 3.4 mm]. High-resolution T₁-weighted structural scans were collected using a Magnetization-Prepared RApid Gradient Echo sequence (MPRAGE; 1 mm × 1 mm × 1 mm).