Epifluorescent images were taken with an inverted Nikon Ti epifluorescence microscope (Nikon Corporation, Chiyoda, Tokyo, Japan) equipped with a Plan Apochromat 60×, 1.4 NA oil immersion objective, an HBO‐100W Lamp, and an IXON X3897 Andor (Belfast, Northern Ireland, UK) camera, operated via the NIS‐Elements AR software (version 4.20; Nikon).
Confocal images were taken using an Abberior microscope operated with Imspector imaging software (Abberior Instruments, Göttingen, Germany). This setup was built on an Olympus IX83 base, equipped with a UPlanSApo 100× oil immersion objective (Olympus Corporation, Shinjuku, Tokyo, Japan) and an EMCCD iXon Ultra camera (Andor, Belfast, Northern Ireland, UK). Pulsed 561‐nm and 640‐nm lasers were used for excitation of ATTO 590 and STAR 580 and Alexa Fluor 647, respectively. For stimulated depletion, lasers emitting at 595 and 775 nm were employed. Where mentioned, images were deconvolved using Huygens software (Scientific Volume Imaging,WWW.svi.nl ).
Confocal images were taken using an Abberior microscope operated with Imspector imaging software (Abberior Instruments, Göttingen, Germany). This setup was built on an Olympus IX83 base, equipped with a UPlanSApo 100× oil immersion objective (Olympus Corporation, Shinjuku, Tokyo, Japan) and an EMCCD iXon Ultra camera (Andor, Belfast, Northern Ireland, UK). Pulsed 561‐nm and 640‐nm lasers were used for excitation of ATTO 590 and STAR 580 and Alexa Fluor 647, respectively. For stimulated depletion, lasers emitting at 595 and 775 nm were employed. Where mentioned, images were deconvolved using Huygens software (Scientific Volume Imaging,