2 amino 2 hydroxymethyl 1 3 propanediol

Manufactured by Merck Group

Sourced in United States

2-amino-2-(hydroxymethyl)-1,3-propanediol is a chemical compound used as a buffer in various laboratory applications. It maintains a stable pH environment and aids in the preservation of biological samples. This product is commonly used in biochemical, molecular biology, and analytical chemistry procedures.

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Lab products found in correlation

4 morpholineethanesulfonic acid, by Merck Group (4 mentions) Methoxy polyethylene glycol amine mpeg nh2, by Laysan Bio (4 mentions) Polyacrylic acid, by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (4 mentions) Phosphate buffered saline (pbs), by Merck Group (4 mentions) Chloroform, by Merck Group (3 mentions) Hydrochloric acid (hcl), by Merck Group (3 mentions) Tetraethyl orthosilicate, by Merck Group (2 mentions) Draq5 fluorescent probe, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Anti cd8α monoclonal antibody clone 2.43, by BioXCell (2 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Merck Group (2 mentions) Streptavidin, by ProSpec (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Trichloroacetic acid, by Merck Group (2 mentions) Toluene, by Thermo Fisher Scientific (2 mentions) Hexane, by Thermo Fisher Scientific (2 mentions) 4 dimethylaminopyridine, by Merck Group (2 mentions) 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride, by Merck Group (2 mentions)

17 protocols using 2 amino 2 hydroxymethyl 1 3 propanediol

Synthesis of Ceramic Nanoparticles

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Tetraethyl orthosilicate (TEOS, ≥ 99.0%), 2-amino-2-(hydroxymethyl)-1,3-propanediol (AHMPD, ≥ 99.9%), cetyltrimethylammonium tosylate (CTATos, ≥ 98.0%), ammonium nitrate (NH₄NO₃), ferric chlorid (99%) and squalane (96%) were obtained from Sigma–Aldrich (Lyon, France). Nitric acid 65% (HNO₃) was purchased from Carlo-Erba (Barcelona, Spain). Sodium stearate (98.8%) was obtained from TCI (Tokyo, Japan). Oleic acid (99%) was purchased from Alfa Aesar (Lancashire, UK) while dibenzylether (DBE, 99%) was purchased from Acros Organic (Waltham, Massachusetts, USA).

Adam A., Parkhomenko K., Duenas-Ramirez P., Nadal C., Cotin G., Zorn P.E., Choquet P., Bégin-Colin S, & Mertz D. (2021). Orienting the Pore Morphology of Core-Shell Magnetic Mesoporous Silica with the Sol-Gel Temperature. Influence on MRI and Magnetic Hyperthermia Properties. Molecules, 26(4), 971.

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Rare-earth Nanoparticle Synthesis and Functionalization

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Rare-earth(III) acetate hydrate (RE: Yb, Er, Ce), zinc acetate, sodium trifluoroacetate, oleic acid (OA), 1-octadecene (ODE), sodium hydroxide, ammonium fluoride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Poly(acrylic acid) (PAA; average Mw 1800), poly(maleic anhydride-alt-1-octadecene) (PMH; 30–50 kDa), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), cyclohexane, chloroform, 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base), fetal bovine serum (FBS), phosphate buffered saline (PBS), and RPMI-1640 medium were purchased from Sigma-Aldrich and used without further purification. Yttrium(III) oxides and trifluoroacetic acid (99%) were purchased from Alfa Asea and used as received. Methoxy polyethylene glycol amine (mPEG-NH₂; 5000 m.W.) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH₂; 40 kDa) was purchased from Advanced Biochemicals. Anti-PD-L1 monoclonal antibody (Atezolizumab, Clone SP142) was purchased from Selleckchem. Anti-CD8α monoclonal antibody (Clone 2.43) was purchased from Bio X Cell. EZ-Link™ Sulfo-NHS-LC-Biotin and DRAQ5 Fluorescent Probe were purchased from Thermo Fisher Scientific. Streptavidin was purchased from ProSpec. Y(CF₃COO)₃ was prepared by the literature method.⁵¹ Detailed information on the antibodies is included in the Life Sciences Reporting Summary.

Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.

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Ginsenoside-Rg5 Cytotoxicity and Genotoxicity

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Ginsenoside-Rg5 (purity>95%) was provided by Dr WM Zhao at the Shanghai Institute of Materia Medica of the Chinese Academy of Sciences (Shanghai, China). Dimethylsulfoxide, Trypan blue, low melting agarose, 4′,6-diamidino-2-phenylindole (DAPI), normal melting agarose, 2-amino-2-(hydroxymethyl)-1,3-propanediol, sodium dodecyl sulfate, ethylenediaminetetraacetic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), N-methyl-N-nitro-N-nitrosoguanidine (MNNG, inducing DNA damage), Tween 20 and paraformaldehyde were obtained from Sigma-Aldrich. Propidium iodide (PI) (21 (link)) and DMEM were obtained from Gibco Life Technologies (Carlsbad, CA, USA). The Apoptosis Detection kit I was obtained from BD Pharmingen San Diego, CA, USA). Triton X-100, ethidium bromide, FBS, xylene cyanol and bromphenol blue were obtained from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China).

LIANG L.D., HE T., DU T.W., FAN Y.G., CHEN D.S, & WANG Y. (2014). Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells. Molecular Medicine Reports, 11(2), 940-946.

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Monocrystalline Si Cantilever Arrays

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Octosensis microcantilever arrays (chips
with eight monocrystalline Si cantilevers, 500 μm in length)
were obtained from Micromotive GmbH (Mainz, Germany). The following
chemicals were purchased from Sigma-Aldrich and used as received without
further purification: isopropanol, (3-glycidyloxypropyl)trimethoxysilane
(98%); toluene (99.8%); N^α,N^α-bis(carboxymethyl)-l-lysine
hydrate (NTA–NH₂) (97%); 1-[(3-dimethylamino)propyl]-3-ethylcarbodiimide
methiodide (EDC); N-hydroxysuccinimide (NHS), 2-(N-morpholino)ethanesulfonic acid (MES); 2-amino-2-(hydroxymethyl)-1,3-propanediol
(Tris base); carbonate/bicarbonate buffer; and phosphate buffered
saline (PBS, pH 7.4). Sodium chloride was purchased from Panreac (Spain).
BSA (fraction V) and LYS were purchased from BioLabs (England) and
Fluka, respectively. The Omp2a protein was expressed, purified, and
refolded using a previously reported procedure.^{22 (link),23 (link)}

Lopes-Rodrigues M., Puiggalí-Jou A., Martí-Balleste D., del Valle L.J., Michaux C., Perpète E.A, & Alemán C. (2018). Thermomechanical Response of a Representative Porin for Biomimetics. ACS Omega, 3(7), 7856-7867.

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Synthesis of Silica Nanoparticles via Sol-Gel

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Titanium (IV) tetrachloride (99.9% trace metals basis), benzyl alcohol (puriss., 99–100.5% (GC)), 2‐amino‐2‐(hydroxymethyl)‐1,3‐propanediol (Trizma base, puriss., ≥99.7%), chloroform (≥99.8%), diethyl ether (for HPLC, ≥99.9%, inhibitor‐free), ethanol (absolute), tetraethylorthosilicate (TEOS, 98%), methyl orange (MO), and acetaminophen (paracetamol, ≥99%) were purchased from Sigma‐Aldrich. Hydrochloric acid 37% was obtained from Carlo Erba. Decahydronaphthalene (decalin, a mixture of cis and trans isomers, 98%) and sorbitan monooleate surfactant (Span 80, HLB: 4.3) were obtained from Merck. 3‐Glycidyloxypropyl)trimethoxysilane (GPTMS, Xiameter OFS‐6040, >98.5%) was supplied by Dow. All chemicals were used without further purification.

Marques A.C., Vale M., Vicente D., Schreck M., Tervoort E, & Niederberger M. (2021). Porous Silica Microspheres with Immobilized Titania Nanoparticles for In‐Flow Solar‐Driven Purification of Wastewater. Global Challenges, 5(5), 2000116.

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Protein Expression and Purification

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Ultra-pure dioxane, non-deuterated and deuterated trifluoroethanol (TFE), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), L-alanine, pyridoxal 5′-phosphate, glyoxylate, trichloroacetic acid, D₂O, lactate dehydrogenase (from rabbit muscle), and TSP (3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic sodium salt) were from Sigma (Madrid, Spain). Ampicillin and isopropyl β-d-1-thiogalactopyranoside (IPTG) were from Canvax Biotech (Valladolid, Spain).

Neira J.L., Naganathan A.N., Mesa-Torres N., Salido E, & Pey A.L. (2022). Phosphorylation of Thr9 Affects the Folding Landscape of the N-Terminal Segment of Human AGT Enhancing Protein Aggregation of Disease-Causing Mutants. Molecules, 27(24), 8762.

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Biofunctionalized Nanoparticle Synthesis

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Lead (II) chloride (PbCl₂), 1-octadecene (ODE), cadmium oxide (CdO) and sulfur powder (sublimed) were purchased from Alfa Aesar. Oleylamine, oleic acid, 1-octadecene, poly(maleic anhydride-alt-1-octadecene) (PMH; average molecular weight: 30k-50k), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), poly(acrylic acid) (PAA; average molecular weight: 1800), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base) were purchased from Sigma-Aldrich. Hexane, toluene, chloroform, and DI water were purchased from Fisher Scientific. Methoxy polyethylene glycol amine (mPEG-NH₂; average molecular weight: 5 kD) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH₂‧HCl; average molecular weight: 40 kDa) was purchased from Advanced Biochemicals. All the chemicals were used without further purification. 50 μm capillaries were bought from VitroCom. Purified anti-mouse/human PNAd antibody (Clone: MECA-79), purified anti-mouse CD169 antibody (Clone: 3D6.112) and DRAQ7 (# 424001) were purchased from BioLegend. Anti-mouse CD3 antibody (Clone: KT3) was bought from Bio X Cell.

Wang F., Ren F., Ma Z., Qu L., Gourgues R., Xu C., Baghdasaryan A., Li J., Zadeh I.E., Los J.W., Fognini A., Qin-Dregely J, & Dai H. (2022). In vivo Non-Invasive Confocal Fluorescence Imaging Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors. Nature nanotechnology, 17(6), 653-660.

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Agarose Gel Electrophoresis of DNA

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What man Grade 1 cellulose paper, HCl, acetic acid, and agarose were purchased from Fisher Scientific (Walthman, MA). Single-stranded DNA (ssDNA, 5’-AGT CAG TGT GGA AAA TCT CTA GC-Cy5-3’) was ordered from Integrated DNA Technologies (Coralville, IA) and purified by HPLC. The 100 bp dsDNA ladder was from New England BioLabs (Ipswich, MA). The following chemicals were from Sigma-Aldrich (St. Louis, MO) and used as received: 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris base), 2-aminoethanesulfonic acid (taurine), Ru(bpy)₃Cl₂, ethidium bromide (EtBr) solution (10 mg/mL), and EDTA.

Li X., Luo L, & Crooks R.M. (2015). Low-Voltage Paper Isotachophoresis Device for DNA Focusing. Lab on a chip, 15(20), 4090-4098.

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Development of DNA Aptamer Library

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Sodium chloride, magnesium chloride, potassium chloride, calcium chloride, 2-amino-2-(hydroxymethyl)-1,3-propanediol (Trizma base), hydrochloric acid, 2,5-dimethyl-4-hydroxy-3(2H)-furanone (furaneol), 5-Ethyl-3-hydroxy-4-methyl-2(5H)-furanone (maple furanone), 4,5-Dimethyl-3-hydroxy-2,5-dihydrofuran-2-one (sotolon), 5-Ethyl-4-hydroxy-2-methyl-3(2H)-furanone (homofuraneol), 2-Methoxyphenol (guaiacol), and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) were purchased from Sigma-Aldrich (Munich, Germany). Taq DNA polymerase, dNTPs solution, TAE and TBE electrophoresis buffers, DNA extraction kits, DNA cloning kit, Exonuclease I, and the Escherichia coli DH5α strain were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SYBR Gold dye was purchased from Invitrogen (Thermo Fisher Scientific). SYBR Green I dye was obtained from Lumiprobe Ltd., (Moscow Russia). Streptavidin-coated magnetic beads were supplied by New England Biolabs (Ipswich, MA, USA).
The single-stranded DNA library B1_bank was synthesized by DNA Synthesis Ltd., Moscow, Russia. All primers for DNA amplification were ordered from the DNA Synthesis Ltd., Moscow, Russia. The B1_cap was either purchased from DNA Synthesis Ltd., Moscow, Russia or from Microsynth AG (Balgach, Switzerland). Candidate aptamer sequences were synthesized by Evrogen Ltd. (Moscow, Russia).

Komarova N., Andrianova M., Glukhov S, & Kuznetsov A. (2018). Selection, Characterization, and Application of ssDNA Aptamer against Furaneol. Molecules, 23(12), 3159.

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Biochemical Reagents and Solutions

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Potassium chloride (KCl), glucose, sucrose, and 2-Amino-2-(hydroxy methyl)-1,3-propane diol (Tris), hydroxy-pyrene-1,3,6-trisulfonic acid (Pyranine), guanine-5-diphosphate (GDP) and guanine-5-triphosphate (GTP) were purchased from Sigma-Aldrich. n-Dodecyl-beta-D-maltoside (DDM) was purchased from Anatrace. Ultrapure water was produced from a Milli-Q unit (Millipore, conductivity below 18 MΩ cm).

Tolosa-Díaz A., Almendro-Vedia V.G., Natale P, & López-Montero I. (2020). The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism. Biomolecules, 10(7), 1085.

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