Bovine ovaries were collected from the slaughterhouse and transported to the laboratory within 2 h at 30–35 °C. Cumulus-oocyte complexes (COCs) were collected from 2–8 mm follicles by aspiration with an 18-gauge needle. COCs were washed and those with at least 3 layers of cumulus cells were chosen for the experiment. The IVM medium contained TCM199 (Gibco, Life Technology, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone; Gibco, Life Technology, Carlsbad, CA, USA), 1 µg/mL estradiol, 40 ng/mL insulin-like growth factor (IGF, Sigma-Aldrich, St. Louis, MO, USA), 10 µg/mL follicle-stimulating hormone (FSH, Sigma-Aldrich, St. Louis, MO, USA), 50 ng/mL epidermal growth factor (EGF, Sigma-Aldrich, St. Louis, MO, USA) and 10 µg/mL lutrinizing hormone (LH, Sigma-Aldrich, St. Louis, MO, USA).
Insulin like growth factor igf
Manufactured by Merck Group
Sourced in United States
Insulin-like growth factor (IGF) is a laboratory equipment used to measure the levels of insulin-like growth factors in biological samples. IGF is a protein that plays a crucial role in cell growth and development.
Automatically generated - may contain errors
Lab products found in correlation
Tcm 199, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid 2 phosphate aa2p, by Merck Group (1 mentions)
Dmem f12 ham 1 1, by Merck Group (1 mentions)
Stabilized antibiotic antimycotic solution 100, by Merck Group (1 mentions)
Its x, by Thermo Fisher Scientific (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
2 protocols using insulin like growth factor igf
1
Bovine Oocyte In Vitro Maturation
2
Culturing Confluent Perichondrial Cells
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The confluent perichondrial cells were dissociated into single cells with 0.25%
trypsin. Next, 50,000 cells were seeded in a 100 mm dish containing Dulbecco’s
Modified Eagle’s Medium (DMEM)/F12 Ham (1:1) (Sigma-Aldrich), stabilized
antibiotic–antimycotic solution (100×) (Sigma-Aldrich), 0.2 mM ascorbic acid
2-phosphate (AA2P) (Sigma-Aldrich), 10−7 M dexamethasone
(Sigma-Aldrich), 1× ITS-X (Gibco), 5 ng/mL insulin-like growth factor (IGF)
(Sigma-Aldrich), 10 ng/mL basic fibroblast growth factor (FGF) (Wako), 10 ng/mL
platelet-derived growth factor BB (PDGF-BB) (Peprotech), and 2% FBS. The medium
was changed every other day until the cells became confluent, which occurred
between 7 and 10 days of culture (passage 1 cells).
trypsin. Next, 50,000 cells were seeded in a 100 mm dish containing Dulbecco’s
Modified Eagle’s Medium (DMEM)/F12 Ham (1:1) (Sigma-Aldrich), stabilized
antibiotic–antimycotic solution (100×) (Sigma-Aldrich), 0.2 mM ascorbic acid
2-phosphate (AA2P) (Sigma-Aldrich), 10−7 M dexamethasone
(Sigma-Aldrich), 1× ITS-X (Gibco), 5 ng/mL insulin-like growth factor (IGF)
(Sigma-Aldrich), 10 ng/mL basic fibroblast growth factor (FGF) (Wako), 10 ng/mL
platelet-derived growth factor BB (PDGF-BB) (Peprotech), and 2% FBS. The medium
was changed every other day until the cells became confluent, which occurred
between 7 and 10 days of culture (passage 1 cells).
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