Flow count

Manufactured by Bioscan

Sourced in United States

The Flow-Count is a versatile laboratory instrument designed for accurate and reliable cell counting. It utilizes the principle of flow cytometry to rapidly enumerate a wide range of cell types, including mammalian, bacterial, and yeast cells. The device precisely measures the number of cells within a given sample volume, providing researchers with essential data for their studies.

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Lab products found in correlation

1515 isocratic hplc pump, by Waters Corporation (1 mentions) Flowstar lb 513, by Berthold Technologies (1 mentions) Omni th tissue homogenizer, by Omni International (1 mentions) Sec 3000 column, by Phenomenex (1 mentions) Sep pak c18 light cartridge, by Waters Corporation (1 mentions) Biograph mct flow 64 scanner, by Siemens (1 mentions) Micropet r4 rodent scanner, by Siemens (1 mentions) Sep pak accell plus qma, by Waters Corporation (1 mentions) Acrodisc 25 mm syringe filter, by Pall Corporation (1 mentions) Eclipse plus c18, by Agilent Technologies (1 mentions) Ar 2000, by Bioscan (1 mentions)

Close spelling variants of this product

Flow count radiometric detector Flow-count γ-radioactivity detection system NaI(Tl) flow count detector Flow-Count PMT Flow count radiation detector Flow count radio detector

5 protocols using flow count

Quantification and Analysis of Radioactivity

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Example 39

Radioactivity was quantified using a Capintec Radioisotope Calibrator (CRC-712M) ion chamber. Radiochemical incorporation yields were determined by radioTLC. EMD TLC Silica gel 60 plates (10×2 cm) were spotted with an aliquot (1-5 μL) of crude reaction mixture approximately 1.5 cm from the bottom of the plate (baseline). Unless otherwise noted, TLC plates were developed in a chamber containing ethyl acetate until within 2 cm of the top of the plate (front). Analysis was performed using a Bioscan AR-2000 radio-TLC imaging scanner and WinScan software. Radiochemical identity and purity were determined by radioHPLC with a Waters 1515 Isocratic HPLC Pump equipped with a Waters 2487 Dual λ Absorbance Detector, a Bioscan Flow-Count equipped with a NaI crystal, and Breeze software or a Shimadzu LC-10AD binary variable pump equipped with an SPD-10AD single wavelength UV detector, a Carroll-Ramsey 105S-1 single-channel high sensitivity radiation detector, and Clarity software.

To account for immobilized radioactivity (which would not be accounted for by radioTLC), reaction vessels were decanted after quenching and residual and solution radioactivity were separately quantified. In all cases, ≥95% of radioactivity remained in solution.

US10259800B2. Method of fluorination using iodonium ylides (2019-04-16). The General Hospital Corporation [US]. Inventors: Neil Vasdev [US], Benjamin H. Rotstein [US], Huan Steven Liang [US].

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HPLC Analysis and Purification Protocol

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Analytical high performance liquid chromatography (HPLC) was performed on a reverse-phase C18 analytical column (4 µm, 4.6 mm×150 mm; Synergi Hydro; Phenomenex, Macclesfield, UK) with 10 mm guard cartridge, UV 254 nm and flow 1 ml/min. Radiodetection was carried out using a Radiomatic 500R series or a Berthold Flowstar LB513 radiodetector (Berthold Technologies, Harpenden, UK). Preparative HPLC was performed on a reverse-phase C18 semi-preparative column (4 µm, 10 mm×150 mm; Synergi Hydro; Phenomenex) with 10 mm guard cartridge, UV 254 nm and flow 3 ml/min. Radiodetection was carried out using a Bioscan Flowcount (Bioscan Inc, Washington, DC) radiodetector. Analytical and preparative HPLC were run in either 0.1% trifluoroacetic acid in water/0.1% trifluoroacetic acid in acetonitrile or 0.1% trifluoroacetic acid in water/0.1% trifluoroacetic acid in methanol.

Lewis D.Y., Champion S., Wyper D., Dewar D, & Pimlott S. (2014). Assessment of [125I]WYE-230949 as a Novel Histamine H3 Receptor Radiopharmaceutical. PLoS ONE, 9(12), e115876.

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Quantifying 64Cu-TIMP2 Stability in Mouse Brain

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Stability of ⁶⁴Cu-labelled TIMP2 in mouse brain 24 h after intraperitoneal injection of radiotracer (19.27 ± 1.32 MBq) was determined as described previously^{50 (link)} with minor modifications, including the use of a Bioscan Flow-Count radioactivity detector to enable sensitive detection of potential radiometabolites. Briefly, mice were deeply anaesthetized with 2.5% (v/v) Avertin, and perfused with chilled PBS (~30 ml) 24 h after intraperitoneal injection of ⁶⁴Cu-labelled TIMP2. Brain tissue was quickly collected and transferred to a tube containing 750 μl ice-cold 70:30 methanol:sodium phosphate buffer (0.1 M, pH 6.8). Samples were homogenized using an Omni TH tissue homogenizer, and protein precipitates were sedimented by centrifugation (9,400g, 10 min). Each supernatant (100 μμl) was analysed via high-performance liquid chromatography (fitted with a highly sensitive positron detector for radioactivity) using size-exclusion liquid chromatography with a Phenomenex SEC 3000 column (Torrance, California, USA) and sodium phosphate buffer (0.1 mol l⁻¹, pH 6.8) at a flow rate of 1.0 ml min⁻¹. The percentage ratio of intact ⁶⁴Cu-labelled TIMP2 (t_R = 11.5 min) was calculated as (peak area for ⁶⁴Cu-labelled TIMP2/total area of all peaks) × 100. More than 95% of the radioactivity was recovered from the column for the analysed samples.

Castellano J.M., Mosher K.I., Abbey R.J., McBride A.A., James M.L., Berdnik D., Shen J.C., Zou B., Xie X.S., Tingle M., Hinkson I.V., Angst M.S, & Wyss-Coray T. (2017). Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature, 544(7651), 488-492.

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Radiolabeled Theranostic Peptide for PET/CT and PET/MRI Imaging

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All solvents and chemicals purchased from commercial sources were of analytical grade or better and were used without further purification. DX600, NODAGA‐DX600, and DOTA‐DX600 were custom synthesized by ChinaPeptides Co., Ltd (Shanghai, China) or CSBio (San Diego, California). Sep‐Pak Accell Plus QMA and Sep‐Pak C18‐Light cartridges were purchased from Waters (Ireland). Acrodisc 25 mm syringe filter (0.22 µm) was purchased from Pall Corporation (USA). The product was analyzed by radio‐ high performance liquid chromatography (HPLC) (1200, Agilent, USA) equipped with γ detector (Flow‐count, Bioscan, Washington. D.C., USA), using a C18 column (Eclipse Plus C18, 4.5 × 250 mm, 5µm, Agilent, USA). The product purity was also determined using Radio‐TLC (AR 2000, Bioscan, USA) after radiolabeling. The PET/CT imaging studies of small animals were performed on the Mira PET/CT of PINGSENG Healthcare Inc. (Shanghai, China), or microPET R4 rodent scanner (Siemens) and analyzed by ASIProVM. The Clinical PET/CT scans were obtained on a Biograph mCT Flow 64 scanner (Siemens, Erlangen, Germany) with unenhanced low‐dose CT. ⁶⁸Ga‐HZ20 PET/MRI was performed on a hybrid 3.0T PET/MR scanner (uPMR790, UIH, Shanghai, China) in female volunteers.

Zhu H., Zhang H., Zhou N., Ding J., Jiang J., Liu T., Liu Z., Wang F., Zhang Q., Zhang Z., Yan S., Li L., Benabdallah N., Jin H., Liu Z., Cai L., Thorek D.L., Yang X, & Yang Z. (2021). Molecular PET/CT Profiling of ACE2 Expression In Vivo: Implications for Infection and Outcome from SARS‐CoV‐2. Advanced Science, 8(16), 2100965.

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Radiosynthesis and Characterization of [18F]PB0822

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The tosylate
precursor was labeled with [¹⁸F]fluoride with an optimized
condition, using [¹⁸F]KF and K_2.2.2 in acetonitrile
at 100 °C for 10 min with the unprotected benzimidazole. The
radiochemical purity and the identity of the [¹⁸F]PB0822
PET radioligand were characterized by an analytical HPLC system, equipped
with a UV absorption detector (λ = 254 nm) and a radioisotope
detector (Bioscan Flow-Count). The HPLC setup comprises a Phenomenex
Luna 5 μm C18(2) (00G-4252-E0, 100 Å, 250 × 4.6 mm)
column with a typical mobile phase of acetonitrile and ammonium formate
(30:70% of 0.1 M, pH = 6.5) at a flow rate of 1 mL/min. The identity
of [¹⁸F]PB0822 was confirmed by comparing the retention
time with the coinjected and standard compound [¹⁹F]PB0822
(RT = 10.56 min) along with the gamma peak (RT = 10.88 min). [¹⁸F]PB0822 was obtained with a 1.2% yield (nondecay corrected)
at EOS with 99.9% radiochemical purity and with a molar activity of
the radioligand at 965 Ci.mmol^–1.

Behof W.J., Haynes J.R., Whitmore C.A., Cheung Y.Y., Tantawy M.N., Peterson T.E., Wijesinghe P., Matsubara J.A, & Pham W. (2024). Synthesis and Evaluation of a Novel PET Radioligand for Imaging Glutaminyl Cyclase Activity as a Biomarker for Detecting Alzheimer’s Disease. ACS Sensors, 9(5), 2605-2613.

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