Subconfluent cells were incubated with 0.5 mg/ml Alexa 488 10,000 MW dextran (Invitrogen) for 20 min. Then cells were briefly washed 3× with 37°C PBS to remove unbound dextran and immediately fixed with 4% PFA at 37 °C. Membrane impermeable 200 mM Red-40 was added to quench extra cellular dextran signal allowing quantification of only internalized dextran (Schultz et al., 2014 (link)). Cells were immediately imaged on an Olympus IX81 microscope and fluorescent intensity was calculated by ImageJ. Significance was determined by a one-way ANOVA with Tukey post-hoc (Fig. 5C ) analysis or one-way ANOVA with Holm-Sidak’s multiple comparisons post-hoc (Fig. 6B ) comparing each treatment with the Cln3−/− MBEC + vehicle group.
Alexa 488 10 000 mw dextran
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488 10,000 MW dextran is a fluorescent dye-labeled polysaccharide compound. It is used as a molecular weight marker and tracer in various biological and biochemical applications.
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Lab products found in correlation
2 protocols using alexa 488 10 000 mw dextran
1
Quantifying Internalized Dextran via Fluorescent Imaging
2
Quantifying Internalized Dextran via Fluorescent Imaging
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