Three batches of Colagenart® (HC018‐01, HC018‐02, and HC018‐03) were provided by LEMAR S.A.P.I. de C.V. (Mexico City, Mexico). Sodium chloride, and monobasic, dibasic sodium phosphate, and sodium 3‐(trimethylsilyl) tetradeuteriopropionate (TSP) (analytical grade) were acquired from J. T. Baker; (NY, USA). Water, formic acid, and acetonitrile (MS grade) and dimethyl sulfoxide (DMSO) (analytical grade) were acquired from Sigma‐Aldrich (MO, USA). Note that 99.98% deuterated water solution was acquired from Cambridge Isotope Laboratories, Inc. (MA, USA). HepG2 cell line (ATCC® HB‐8065; VA, USA) and CaCo‐2 cell line (ATCC® HTB‐37) were acquired from ATCC (VA, USA). Fetal bovine serum and DMEM medium were obtained from Thermo Fisher Scientific (MA, USA). CellTiter 96® AQueous reagent was acquired from Promega (WI, USA).
Hepg2 cell line atcc hb 8065
Manufactured by American Type Culture Collection
Sourced in United States
The HepG2 cell line (ATCC® HB‐8065) is a human hepatocellular carcinoma cell line derived from the liver tissue of a 15-year-old Caucasian male. The cells exhibit epithelial morphology and are commonly used in cell-based assays for liver-related studies.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Celltiter 96 aqueous reagent, by Promega (1 mentions)
Penicillin streptomycin mix, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Euroclone (1 mentions)
Cholesterol quantitation kit, by Merck Group (1 mentions)
Pcr mix plus, by A&A Biotechnology (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions)
Qubit dsdna hs assay kit, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem with glutamax, by Thermo Fisher Scientific (1 mentions)
2 protocols using hepg2 cell line atcc hb 8065
1
Characterization of Colagenart® Bioactive Compounds
2
Hepatocyte Cell Line Cholesterol Assay
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The Plant Genomic DNA Extract Mini Kit was purchased from Fisher Molecular Biology (Rome, Italy). The Qubit dsDNA HS Assay Kit was purchased from Invitrogen (Carlsbad, CA, USA). The PCR Mix Plus was purchased from A&A Biotechnology (Gdansk, Poland). HepG2 cell line (ATCC® HB-8065™) was purchased from ATCC (Manassas, VA, USA). Dulbecco’s modified Eagle medium (DMEM) with GlutaMAX was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Phosphate-buffered saline (PBS), penicillin–streptomycin mix, dimethyl sulfoxide (DMSO), and Cholesterol Quantitation Kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Euroclone (Milan, Italy). CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega (Madison, WI, USA). The Total Bile Acid Assay Kit (Fluorometric) was purchased from Cell Biolabs San Diego, CA, USA). OriginLab software was purchase from OriginLab Corporation (Northampton, MA, USA).
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