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2 protocols using panc 1

1

Nanomedicine Formulation and Evaluation

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DPPC
(99%) and DOPE (99%)
were purchased from Avanti Polar Lipids Inc (Alabaster, USA). Paclitaxel
(99%) was obtained as a gift sample from Mac-Chem Products. Stearic
acid (>98%) was purchased from Loba Chemie, EDC (99%), and dialysis
membrane-50 was purchased from HIMEDIA, NHS (>97%) was purchased
from
Sisco Research Lab, and rhodamine-6G (>97%), Nile red (>98%),
and
FITC (>90%) were purchased from Sigma-Aldrich and agarose from
MP
Biomedicals. HPLC grade methanol and chloroform were purchased from
Merck, Mumbai (India). Human PDAC cell lines MiaPaCa-2 and Panc-1,
triple negative human breast cancer cell line MDA-MB-231, and head
and neck cancer cell line AW-8507 were procured from National Centre
for Cell Sciences, (NCCS) Pune. Dulbecco’s modified Eagle medium
(DMEM), Dulbecco’s phosphate buffered saline 1×, Trypsin
EDTA solution, and fetal bovine serum were purchased from HIMEDIA.
Degassed water was used in the preparation of tissue mimicking agarose
phantoms for US echogenicity studies.
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2

Pancreatic Cancer Cell Line Cultivation

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BxPC-3, AsPC-1 cells were acquired from the American Type Culture Collection (ATCC, Manassas, VA), MIA PaCa-2 and PANC-1 were from the National Centre for Cell Sciences (Pune, India). PAN 02, a C57BL/6-derived pancreatic cancer cell line was obtained from the National Cancer Institute (Frederick, MD), HDPE cell line was a kind gift from Dr. Florencia McAllister, UTMDACC (Houston, TX) and was grown in keratinocyte serum free medium with 5 ng/ml recombinant human EGF. Cells were cultured in Dulbecco's modified Eagle medium containing 10% fetal bovine serum (FBS) and the antibiotic mixture (penicillin, streptomycin and amphotericin). All cell lines were tested for the presence of mycoplasma and found to be negative.
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