For SEC experiments, 20 μg of Pry1CAP was injected onto a Yarra SEC-2000 column (Phenomenex, Torrance, CA) at flow-rate of 0.5 ml/min with a Shimadzu Prominence series HPLC (Kyoto, Japan) using PBS pH 7.4 as the mobile phase. The elution was monitored with a photo diode array detector (Shimadzu). The system was calibrated using Bio-Rad gel filtration standard (Hercules, CA) consisting of proteins with molecular weights of 670, 158, 44, 17 and 1.35 kDa. Data analysis was performed on the 280 nm wavelength extracted chromatograph using Shimadzu LCsolution version 1.25. Experiments were conducted in triplicate.
Prominence series hplc
Manufactured by Shimadzu
Sourced in Japan
The Prominence series HPLC from Shimadzu is a high-performance liquid chromatography system designed for a wide range of analytical applications. It features advanced technology and precise control for reliable and accurate separation and detection of chemical compounds.
Automatically generated - may contain errors
3 protocols using prominence series hplc
1
Pry1CAP Characterization by SEC
2
Caffeine Metabolism in Bacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 50 bacterial strains sequenced in this study were inoculated from frozen glycerol stocks into a modified M9 minimal medium containing, per liter, 12.8 g Na2HPO4⋅7H2O, 3 g K2HPO4, 2.76 g NH4Cl, 0.5 g NaCl, 493 mg MgSO4, 55.5 mg CaCl2, and 2.5 g caffeine (J. T. Baker, Avantor Performance Materials, Inc., Center Valley, PA, United States) or in nutrient broth (6 g/L peptone and 3 g/L yeast extract; VWR International LLC, Radnor, PA, United States) containing 2.5 g/L caffeine. Aliquots were removed periodically from the cultures and mixed 1:1 (vol:vol) with methanol to stop cell growth and metabolism. Concentrations of caffeine and metabolites were then determined by HPLC as described previously (Summers et al., 2012 (link)) using a Shimadzu Prominence series HPLC equipped with a photodiode array. Metabolites were identified based on their retention times and UV spectra when compared with authentic standards.
3
HPLC Characterization of Cynara scolymus Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High performance liquid chromatography (Shimadzu Prominence series HPLC) was used to determine the chemical composition of each extract obtained. The HPLC equipment comprised of a DGU-20A5 degasser, LC-20AD pump, SIL-20A injector, CTO-20AC oven, SPD-M20A detector, HiChrome C18 250x4mm 5 µm column and a CBM-20Alite controller.
The data was analysed using Shimadzu LCsolution version 1.23 software.
The analytical method was adapted from British Pharmacopeia [28] , the mobile phase was 0.5% phosphoric acid in water (solvent A) and 0.5% phosphoric acid in acetonitrile (solvent B) at a flow rate of 1.2 mL/min. The elution profile is given in Table 2.
Chromatograms were recorded at 330 nm and CA quantification was carried out using a standard calibration curve of CA with the above mentioned HPLC method.
All data presented herein are average values ± standard deviation of three independent experiments and expressed as mg/5g dried leaves of Cynara scolymus L. leaves. The statistical significance was evaluated using independent-samples t-tests which were conducted using SPSS 17 and a P value of less than 0.05 was considered to be significant.
The data was analysed using Shimadzu LCsolution version 1.23 software.
The analytical method was adapted from British Pharmacopeia [28] , the mobile phase was 0.5% phosphoric acid in water (solvent A) and 0.5% phosphoric acid in acetonitrile (solvent B) at a flow rate of 1.2 mL/min. The elution profile is given in Table 2.
Chromatograms were recorded at 330 nm and CA quantification was carried out using a standard calibration curve of CA with the above mentioned HPLC method.
All data presented herein are average values ± standard deviation of three independent experiments and expressed as mg/5g dried leaves of Cynara scolymus L. leaves. The statistical significance was evaluated using independent-samples t-tests which were conducted using SPSS 17 and a P value of less than 0.05 was considered to be significant.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!