All bacterial isolates were identified using colony morphologic analysis and Gram staining. Isolate identification and antimicrobial susceptibility were confirmed using the MicroScan WalkAway-96 SI system (Beckman Coulter, Inc., Brea, CA, USA). Results were interpreted according to the 2018 Clinical and Laboratory Standards Institute guidelines [9 ]. After screening for drug susceptibility, the presence of extended-spectrum β-lactamase (ESBL)-producing bacteria was confirmed by the disk diffusion method using clavulanate [9 ]; in contrast, the presence of AmpC-producing bacteria was confirmed by the disk diffusion method using boronic acid [10 (link)]. Carbapenem-resistant Enterobacteriaceae (CRE) were determined according to the standards of the Ministry of Health, Labour, and Welfare of Japan [11 ] for meropenem (minimum inhibitory concentration [MIC] of meropenem: ≥2 μg/mL), imipenem (MIC of imipenem: ≥2 μg/mL), and cefmetazole (MIC of cefmetazole: ≥64 μg/mL) susceptibility.
Microscan walkaway 96 si
Manufactured by Beckman Coulter
Sourced in United States
The MicroScan WalkAway-96 SI is an automated microbiology identification and susceptibility testing system. It is designed to perform identification and antimicrobial susceptibility testing of a wide range of clinically relevant microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using microscan walkaway 96 si
1
Bacterial Identification and Antimicrobial Susceptibility
2
Antimicrobial Resistance Profiling of E. coli
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All E. coli isolates were identified by a colony morphologic analysis, gram staining, and Triple Sugar Iron Agar. Isolate identification was confirmed using the MicroScan WalkAway-96 SI (Beckman Coulter, Brea, USA). The minimum inhibitory concentrations (MICs) were also determined using the MicroScan WalkAway-96 SI. The results of the period from January 2011 to June 2013 were interpreted in accordance with the 2009 Clinical and Laboratory Standards Institute (CLSI) breakpoints (17 ), and the results of the period from July 2013 to June 2015 were interpreted in accordance with the 2011 CLSI breakpoints (18 ). The production of ESBL was screened by measuring the MICs of cefotaxime, ceftazidime, and aztreonam.
Confirmational testing was performed using an Ambler class C & ESBL Identification Set (Kanto Chemical, Tokyo, Japan). All plates were incubated at 35°C for 24 hours.
Confirmational testing was performed using an Ambler class C & ESBL Identification Set (Kanto Chemical, Tokyo, Japan). All plates were incubated at 35°C for 24 hours.
3
Microbial Identification and Antibiotic Susceptibility
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolate identification and antimicrobial susceptibility were confirmed using the MicroScan WalkAway-96 SI (Beckman Coulter, Brea, USA). The minimum inhibitory concentrations were also determined using the MicroScan WalkAway-96 SI. The results were interpreted according to the 2018 Clinical and Laboratory Standards Institute breakpoints (12 ).
4
Identification and Antimicrobial Susceptibility of ESBL-PE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All ESBL-PE isolates were identified via colony morphologic analysis and gram staining. Isolate identification and antimicrobial susceptibility were confirmed using the MicroScan WalkAway-96 SI (Beckman Coulter, Inc., Brea, CA, USA). The MICs were determined using a dilution antimicrobial susceptibility test in accordance with the manufacturer's instructions (Eiken Chemical, Japan). All plates were incubated at 35°C overnight (16-20 hours) . The results were interpreted according to the 2016 CLSI breakpoints.
5
Enterococcus Antimicrobial Susceptibility Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All E. faecalis and E. faecium isolates were identified by colony morphology analysis and gram staining. Isolate identification was confirmed using a MicroScan WalkAway-96 SI (Beckman Coulter, Inc., Brea, CA, USA). The MICs were determined using a dilution antimicrobial susceptibility test according to the manufacturer's instructions (Eiken Chemical, Japan). Daptomycin sensitivity was assessed using Mueller-Hinton broth supplemented with 50 µg/mL calcium. All plates were incubated overnight at 35°C (vancomycin: 24 h, others: 16-20 h). The results were interpreted according to the 2016 CLSI breakpoints.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!