Metamorph metafluor software

For microscopic observations, conidia were inoculated onto pre-cleaned glass coverslips overlaid with liquid media. To observe co-localization of GFP-AkrA and mRFP-PH^OSBP, strain ZYA13 (S1 Table) was cultured at 37°C for 10 h in non-inducing medium (non-inducing conditions for the alcA(p) driving expression of AkrA) and shifted for 6 h to the inducing medium (in which the alcA promoter was induced) before microscopic observation [34 (link)]. Differential interference contrast (DIC) and fluorescence images of the cells were captured with a Zeiss Axio imager A1 microscope (Zeiss, Jena, Germany) equipped with a Sensicam QE cooled digital camera system (Cooke Corporation, Germany). The images were processed with MetaMorph/MetaFluor software (Universal Imaging, West Chester, PA) and assembled in Adobe Photoshop (Adobe, San Jose, CA).

For GFP expression analysis, a batch of 10 flies per conditions and genotypes were analyzed in triplicate and representative individuals are shown. Images were acquired using a high-resolution video camera (CoolSNAP HQ Monochrome, Roper Scientific, Inc), mounted on a Zeiss Stereo Lumar V12 binocular microscope with a Neolumar 0.83 objective. Image acquisition was performed with the Metamorph/Metafluor software (universal Imaging, West Chester, PA). Individuals shown in Figures 
4,
5 and
6 were dissected out their pupal case and mounted in Voltaleff oil 3S prior imaging.