Human NSCLC cell lines (A549, H1299, PC-9, HCC827, and H1975) and the normal human lung epithelial cell line BEAS-2B were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. All NSCLC cell lines were seeded in culture flasks and cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) at 37 °C in the presence of 95% O2 and 5% CO2. BEAS-2B cells were cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM, Gibco) under consistent culture conditions.
Beas 2b
Manufactured by Shanghai Cell Bank
Sourced in China
BEAS-2B is a cell line derived from normal human bronchial epithelial cells. The cells are immortalized and maintain characteristics of normal bronchial epithelial cells. The cell line is commonly used in research applications to study respiratory biology and diseases.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
H1299, by Shanghai Cell Bank (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
H1975, by Shanghai Cell Bank (3 mentions)
Hcc827, by Shanghai Cell Bank (3 mentions)
Beas 2b, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Snu899, by Shanghai Cell Bank (2 mentions)
Hep 2, by Shanghai Cell Bank (2 mentions)
Spc a 1, by Shanghai Cell Bank (2 mentions)
Calu 1, by Shanghai Cell Bank (2 mentions)
Sk mes 1, by Shanghai Cell Bank (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by Shanghai Cell Bank (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Dharmafect 1, by Horizon Discovery (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Amc hn8, by Shanghai Cell Bank (1 mentions)
20 protocols using beas 2b
1
Culturing Human NSCLC and Lung Epithelial Cells
2
Cytotoxic Effects of (-)-Guaiol on Lung Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LUAD cells A549 and Calu-1 and normal lung cells BEAS-2B were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin streptomycin in a humidified atmosphere with 5% CO2 at 37°C. (−)-Guaiol was diluted in methanol at a primary stock concentration of 10 mM. The control group was DMEM with 10% FBS and methanol.
3
Hepatoma Cell Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human hepatoma cell lines,
HUH-7 and Hep3B, and human normal cell lines, HUVEC, BEAS-2B, and
QSG-7701, were obtained from Shanghai Cell Bank of Chinese Academy
of Sciences, China; RPMI-1640 culture medium, fetal bovine serum,
and trypsin were obtained from Thermo Fisher Scientific (China) Co.,
Ltd; MLs, DAPI, CK8/18/19-FITC,24 (link),25 (link) CD45-PE, and magnetic
separation rack were obtained from Huzhou Lieyuan Medical Laboratory
Co., Ltd; anti-GPC3 and anti-EpCAM antibodies were obtained from Abcam;
the Prussian Blue Iron Stain Kit (Nuclear fast red) was obtained from
Beijing Solarbio Science & Technology Co., Ltd; distearoylphosphatidylethanolamine-polyethylene
glycol (DSPE-PEG), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), hexadecyl-quaternized (HQCMC), glycidyl hexadecyl dimethylammonium
chloride (GHDC), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC·HCL), and agarose were obtained
from JuKang (Shanghai) Biotechnology Co., Ltd; cholesterol, hydrochloric
acid, and other common reagents were obtained from Sinopharm Chemical
Reagent Co., Ltd; and the cell proliferation and toxicity test kit
(CCK-8) was obtained from Shanghai Yisheng Biology Co., Ltd.
HUH-7 and Hep3B, and human normal cell lines, HUVEC, BEAS-2B, and
QSG-7701, were obtained from Shanghai Cell Bank of Chinese Academy
of Sciences, China; RPMI-1640 culture medium, fetal bovine serum,
and trypsin were obtained from Thermo Fisher Scientific (China) Co.,
Ltd; MLs, DAPI, CK8/18/19-FITC,24 (link),25 (link) CD45-PE, and magnetic
separation rack were obtained from Huzhou Lieyuan Medical Laboratory
Co., Ltd; anti-GPC3 and anti-EpCAM antibodies were obtained from Abcam;
the Prussian Blue Iron Stain Kit (Nuclear fast red) was obtained from
Beijing Solarbio Science & Technology Co., Ltd; distearoylphosphatidylethanolamine-polyethylene
glycol (DSPE-PEG), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), hexadecyl-quaternized (HQCMC), glycidyl hexadecyl dimethylammonium
chloride (GHDC), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC·HCL), and agarose were obtained
from JuKang (Shanghai) Biotechnology Co., Ltd; cholesterol, hydrochloric
acid, and other common reagents were obtained from Sinopharm Chemical
Reagent Co., Ltd; and the cell proliferation and toxicity test kit
(CCK-8) was obtained from Shanghai Yisheng Biology Co., Ltd.
4
Rab14 modulation in lung cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lung cancer cell lines H460, H358, LK2, A549, H1299, H292 and the normal human bronchial epithelial (HBE) cell line BEAS-2B were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. Cells were cultured in RPMI-1640 medium with fetal bovine serum (FBS, 10%) in an saturated humidity incubator. LK2 cells were maintained in RPMI −1640 medium until the confluence reached 60%. pCMV6-Rab14 plasmid/empty vector was transfected using Lipofectamine 3000 (Invitrogen, USA). The plasmids were constructed by Origene. siGENOME siRNA for Rab14 (Dharmacon, USA) and the non-targeting siRNA were used for knockdown using Dharmafect1 (Dharmacon, USA) according to the manufacturer′s instructions.
5
Culturing Human Laryngeal Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three human laryngeal cancer cell lines (SNU899, AMC-HN-8, and Hep-2) and one normal human bronchial epithelial cell line (BEAS-2B) were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI 1640 medium (Gibco, Los Angeles, CA, U.S.A.) supplemented with 10% fetal bovine serum (FBS) (Sigma–Aldrich, St. Louis, MO, U.S.A.), 100 units/ml penicillin, and 100 μg/ml streptomycin. All the cells were maintained in a humidified incubator with 95% air and a 5% CO2 atmosphere at 37°C.
6
Lung Epithelial Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The normal human lung epithelial cell line Beas-2B, along with the LAC cell lines A549, H1299, GLC-82 and 95D, were all purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China) and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum (FBS) at 37 °C with 5% CO2. The culture medium was changed every 2–3 days according to cell growth. When cell confluence reached 80%–90%, cells were passaged. The two cells with the highest expression of LINC00485 were screened out by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the subsequent experiments.
7
Culturing Human Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549 (originated from adenocarcinoma) and NCI-H292 (mucoepidermoid cancer), two human lung cancer cell lines, and an immortalized normal human lung epithelial cell line BEAS-2B, were obtained from Shanghai Cell Bank (Shanghai, China). The basic medium for A549 and NCI-H292 culture was RPMI (Roswell Park Memorial Institute) 1640 (Gibco, Grand Island, NY, USA), for BEAS-2B was DMEM (Dulbecco’s Modified Eagle Medium, Gibco, Grand Island, NY, USA). All culture media were supplemented with 10% (v/v) FBS (fetal bovine serum) (HyClone, Logan, UT, USA), 100 U/mL penicillin and 100 U/mL streptomycin. All cell lines were maintained at 37 °C with 5% CO2 in a humidified incubator.
8
Cell Line Maintenance Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bronchial epithelial cell line (BEAS-2B) and lung cancer cell lines (H520, H596, A549 and H1299) were provided by Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in Dulbecco′s Modified Eagle′s Medium (DMEM) with 10% fetal bovine serum (FBS) at 37°C, 5% CO2 in a sterile incubator.
9
Culturing Human Laryngeal and Bronchial Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three human laryngeal cancer cell lines (SNU899, TU212, and Hep-2) and one normal human bronchial epithelial cell line BEAS-2B were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, P.R. China). Cells were cultured in RPMI-1640 medium (Gibco, Los Angeles, CA, USA) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin. All the cells were maintained at 37°C in a humidified incubator containing 5% CO2.
10
Cell Culture of Human Lung Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two human normal bronchial or lung epithelial cells (16HBE and BEAS-2B) and five human LUAD cell lines including NCI-H1299, A549, SPC-A1, PC9 and NCI-H1650 were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (China), which were cultured in 1640 medium (Hyclone, Beijing, China) plus 10% fetal bovine serum (FBS; gibco, California, USA) at 37 °C and 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!