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Rabbit polyclonal anti cd3

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Rabbit polyclonal anti-CD3 is an antibody product designed for research use. It is a polyclonal antibody raised in rabbits against the CD3 antigen, which is a component of the T cell receptor complex. This antibody can be used to detect and study T cells in various applications.

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Lab products found in correlation

Rabbit igg, by Abcam (2 mentions) Mouse igg1, by Agilent Technologies (2 mentions) Mouse anti cd68 clone kp1, by Agilent Technologies (2 mentions) Mouse igg2a, by Abcam (2 mentions) Goat polyclonal anti nkp46, by R&D Systems (2 mentions) Mouse anti cd56 clone 123c3, by Agilent Technologies (2 mentions) Mouse anti neutrophil defensins clone d21, by Leica Biosystems (2 mentions) Mouse anti cd16 clone 2h7, by Leica Biosystems (2 mentions) Rabbit anti cd32 clone epr6657 2, by Abcam (2 mentions) Mouse anti cd20 clone l26, by Agilent Technologies (2 mentions) Goat igg, by R&D Systems (2 mentions) Polyclonal rabbit anti vwf, by Agilent Technologies (1 mentions) Polyclonal rabbit anti mouse, by Agilent Technologies (1 mentions) Biotinylated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Rat anti b220, by Thermo Fisher Scientific (1 mentions) Rat anti foxp3, by Thermo Fisher Scientific (1 mentions) Chicken anti c3 c3a, by Abcam (1 mentions) Biotinylated pna, by Vector Laboratories (1 mentions) Mouse anti cd163, by Thermo Fisher Scientific (1 mentions) Mouse anti cd68, by Thermo Fisher Scientific (1 mentions)

9 protocols using rabbit polyclonal anti cd3

1

Antibodies and Conjugates for PUUV Detection

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We made use of the following antibodies and conjugates: polyclonal rabbit anti- VWF, HRP labeled polyclonal goat anti-rabbit IgG and polyclonal rabbit anti-mouse (All from Dako, the Netherlands). FITC labeled monoclonal anti-CD31 (Sigma Aldrich, USA), polyclonal rabbit anti-CD41 (Perbio Science, the Netherlands), polyclonal rabbit anti-CD3 (Dako), polyclonal rabbit anti-PUUV nucleoprotein (BEI Resources, USA), monoclonal anti-PUUV glycoprotein (HY Test, Finland), monoclonal anti-ανβ3 integrin (Abcam, UK), monoclonal anti-vitronectin (Novus Bio, USA), polyclonal rabbit-anti PAI-1 (Bio Connect, the Netherlands), polyclonal rabbit anti-tissue factor (TF; Bio Connect), human serum from a recovered PUUV case described in Goeijenbier et al. (2011) (link) retrieved after informed consent and ethical board approval. Antibodies and conjugate were diluted in dilution buffer, which consisted of PBS with 0.5% bovine serum albumin, 2% NaCl and 1% normal goat serum.
Goeijenbier M., Meijers J.C., Anfasa F., Roose J.M., van de Weg C.A., Bakhtiari K., Henttonen H., Vaheri A., Osterhaus A.D., van Gorp E.C, & Martina B.E. (2015). Effect of Puumala hantavirus infection on human umbilical vein endothelial cell hemostatic function: platelet interactions, increased tissue factor expression and fibrinolysis regulator release. Frontiers in Microbiology, 6, 220.
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2

Immunofluorescence Analysis of T and B Cells

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Sample preparation and immunofluorescence analysis was described earlier (Vaeth et al., 2014 (link)). The following primary antibodies were used: rat anti-Foxp3, rat anti-B220 (both eBioscience), polyclonal rabbit anti-CD3 (Dako), chicken anti-C3/C3a (Abcam), and/or biotinylated PNA (Vector Laboratories). Anti-CD3 and anti-Foxp3 antibodies were amplified using biotinylated goat anti-rabbit IgG (Thermo Scientific) or biotinylated goat anti-rat IgG (Molecular Probes). Images were captured using a laser-scanning confocal microscope (Leica TCS SP5 II equipment) and analyzed using ImageJ (NIH). A list of antibodies and detailed procedures with dilutions of antibodies and staining of HEp-2 cells can be found in the Supplemental Experimental Procedures.
Vaeth M., Eckstein M., Shaw P., Kozhaya L., Yang J., Berberich-Siebelt F., Clancy R., Unutmaz D, & Feske S. (2016). Store-operated Ca2+ entry in follicular T cells controls humoral immune responses and autoimmunity. Immunity, 44(6), 1350-1364.
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3

Comprehensive Immunohistochemical Analysis of Lymph Nodes

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Immunohistochemistry was performed as previously indicated [73 (link), 82 (link)] on formalin-fixed paraffin-embedded (FFPE) LNs obtained at necropsy. Briefly, sections were deparaffinized and antigen retrieval was performed using a Retriever (Electron Microscopy Services, Hatfield, PA) in Tris-EDTA-Tween-80 buffer 73. Sections were stained for Tcells/B cells/dendritic cells (polyclonal rabbit anti-CD3, Dako, Santa Clara, CA; polyclonal rabbit anti-CD20, Thermo Fisher Scientific, Pittsburgh, PA; mouse-anti-CD11c, Leica Microsystems, Buffalo Grove, IL), macrophage subsets (mouse anti-CD68, Thermo Fisher; rabbit anti-DC-SIGN, ProSci Inc, Poway, CA; mouse anti-CD163, Thermo Fisher), LN vascular and structural aspects (Goat anti-LYVE-1, R&D Systems, Minneapolis, MN; rat-anti PNAd, BioLegend, San Diego, CA), and LN conduit systems (visualized by staining for rabbit anti-collagen 1 [Abcam, Cambridge, MA]). Primary antibodies were visualized with species- and isotype-specific secondary antibodies purchased from Jackson ImmunoResearch (West Grove, PA). Auramine rhodamine was performed as previously indicated [73 (link)] using reagents from BD Biosciences (San Jose, CA). Images were acquired at 20x magnification with a Nikon e1000 widefield microscope (Nikon, Melville, NY) with Nikon Elements.
Ganchua S.K., Cadena A.M., Maiello P., Gideon H.P., Myers A.J., Junecko B.F., Klein E.C., Lin P.L., Mattila J.T, & Flynn J.L. (2018). Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques. PLoS Pathogens, 14(11), e1007337.
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4

Quantifying CD3+ T Cells in FFPE Tissue

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CD3+ T cells were identified in FFPE frontal cortex tissue section (6 μm) by chromogenic immunohistochemistry analysis using anti-CD3 (rabbit polyclonal, 1:100; DAKO), as previously described 3 (link), 6 (link). Whole tissue samples were imaged (VS120 slide scanner Olympus, Shinjuku, Japan) and CD3+ T cells (relative to total nuclei) were quantified using HALO imaging software (Indica Labs v3.3).
Cochrane C.R., Angelovich T.A., Byrnes S.J., Waring E., Guanizo A.C., Trollope G.S., Zhou J., Vue J., Senior L., Wanicek E., Eddine J.J., Gartner M.J., Jenkins T.A., Gorry P.R., Brew B.J., Lewin S.R., Estes J.D., Roche M, & Churchill M.J. (2022). Intact HIV Proviruses Persist in the Brain Despite Viral Suppression with ART. Annals of Neurology, 92(4), 532-544.
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5

Immunohistochemical Analysis of Oropharyngeal SCC

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Tumor tissue samples from oropharyngeal squamous cell carcinomas were fixed in buffered formalin and paraffin embedded. Sections were cut at 4 μm. The following antibodies were used in this study: Anti-CD68 (clone KP1, DAKO, dilution 1:3000), anti-CD3 (rabbit polyclonal, DAKO, dilution 1:100), and anti-Foxp3 (clone 259D/C7, BD Pharmingen, dilution 1:20). Antigen retrieval and staining were performed fully automated in a Ventana Benchmark Ultra according to the manufacturer’s instructions.
Ljokjel B., Haave H., Lybak S., Vintermyr O.K., Helgeland L, & Aarstad H.J. (2022). Tumor Infiltration Levels of CD3, Foxp3 (+) Lymphocytes and CD68 Macrophages at Diagnosis Predict 5-Year Disease-Specific Survival in Patients with Oropharynx Squamous Cell Carcinoma. Cancers, 14(6), 1508.
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6

Immunohistochemical Profiling of Immune Cells

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Antigen-retrieval and signal detection were performed as previously described 20 (link). The following primary Abs were used: goat polyclonal anti-NKp46 (R&D Systems), mouse anti-CD56 (clone 123C3, Dako), mouse anti-CD68 (clone KP1, Dako), mouse anti-neutrophil defensins (clone D21, Leica Biosystems), mouse anti-CD16 (clone 2H7, Leica Biosystems), rabbit anti-CD32 (clone EPR6657(2), Abcam), mouse anti-CD20 (clone L26, Dako), rabbit polyclonal anti-CD3 (Dako). The following control Abs were used: mouse IgG1 (Dako), goat IgG (R&D Systems), mouse IgG2a (Abcam) and rabbit IgG (Abcam).
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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7

Immunohistochemical Profiling of Immune Cells

Cited 1 time
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Antigen-retrieval and signal detection were performed as previously described 20 (link). The following primary Abs were used: goat polyclonal anti-NKp46 (R&D Systems), mouse anti-CD56 (clone 123C3, Dako), mouse anti-CD68 (clone KP1, Dako), mouse anti-neutrophil defensins (clone D21, Leica Biosystems), mouse anti-CD16 (clone 2H7, Leica Biosystems), rabbit anti-CD32 (clone EPR6657(2), Abcam), mouse anti-CD20 (clone L26, Dako), rabbit polyclonal anti-CD3 (Dako). The following control Abs were used: mouse IgG1 (Dako), goat IgG (R&D Systems), mouse IgG2a (Abcam) and rabbit IgG (Abcam).
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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8

Histological and Immunohistochemical Analysis of Colon Cancer

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Paraffin-embedded sections (3-μm-thick) of the colon wall were prepared and stained with H&E for histological analysis. Cross-sections of colon wall were routinely cut in ApcMin/+ mice and AOM/DSS-treated mice. Horizontal sections of colon wall were prepared in AOM alone-treated mice and the number of intramucosal microadenomas was counted on the H&E-stained section.23 (link) Immunohistochemical analyses were performed as described previously using the following primary antibodies: rabbit polyclonal anti-IDO (provided by K. Saito, Kyoto University),24 (link) rabbit polyclonal anti-Foxp3 (Abcam, Cambridge, UK), rabbit polyclonal anti-CD3 (DAKO, Glostrup, Denmark) and rat monoclonal anti-CD45R/B220 (clone RA3-6B2; BD Biosciences, San Jose, CA, USA). Double immunostaining for IDO and CD11c was performed as described previously with a slight modification.16 (link) Ethical approval for use of archival tissues of human CRC was provided by the Ethics Committee of Gifu University School of Medicine.
Takamatsu M., Hirata A., Ohtaki H., Hoshi M., Ando T., Ito H., Hatano Y., Tomita H., Kuno T., Saito K., Seishima M, & Hara A. (2015). Inhibition of indoleamine 2,3-dioxygenase 1 expression alters immune response in colon tumor microenvironment in mice. Cancer Science, 106(8), 1008-1015.
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9

Quantifying Immune Cell Populations in Tissue

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Immunohistochemical staining was performed according to previously described methods47 (link) using the following primary antibodies: mouse monoclonal anti-Foxp3 (Abcam, Cambridge, UK), rabbit polyclonal anti-CD3 (Dako, Glostrup, Denmark), mouse monoclonal anti-rat CD68 (Serotec, Oxford, UK), and rabbit polyclonal anti-collagen type I (Abcam). FOXP3-, CD3- and CD68-positive cells, as well as areas positive for α-SMA and collagen type I staining, were assessed using ImageJ software (version 1.53 s, NIH) by examining five randomly selected fields (100× magnification) of the cortex.
Kurawaki S., Nakashima A., Ishiuchi N., Kanai R., Maeda S., Sasaki K, & Masaki T. (2024). Mesenchymal stem cells pretreated with interferon-gamma attenuate renal fibrosis by enhancing regulatory T cell induction. Scientific Reports, 14, 10251.
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