The three cell lines used were all cultured in conformity with the sterile technique and the standard mammalian cell culture protocols under a 5% CO2 atmosphere at 37 °C.
Lymphocyte cell line (IST-EBV-TW6B) was purchased from the cell bank IRCCS AOU San Martino IST (Italy). Cells were cultured in advanced RPMI 1640 culture medium (Gibco) with 20% of heat inactivated fetal bovine serum (FBS, Gibco), 1% penicillin/streptomycin (P/S, Sigma) and 1% ofl -Glutamine 200 mM (Lonza) in 75 cm2 not treated cell culture flasks (Corning) maintaining the cell density between 9 × 104–5 cells/mL.
Daudi cells (ATCC® CCL213™), originating from a Burkitt’s lymphoma patient, were obtained from American Type Culture Collection (ATCC). Cells were grown in RPMI 1640 culture medium (ATCC) supplemented with 10% of heat inactivated FBS (ATCC), 1% P/S (Sigma) in 75 cm2 not treated cell culture flasks (Corning) with a cell density between 3 × 105–6 cells/mL.
HL60 cells (ATCC® CCL-240™), from an acute myeloid leukemia patient, were purchased from ATCC. They were maintained in Iscove’s Modified Dulbecco’s Medium (Sigma) with 20% heat inactivated FBS (Sigma), 1% Glutamine (Sigma), 1% P/S (Sigma) in 75 cm2 not treated cell culture flasks (Corning), adjusting cell density to 1 × 105–6 cells/mL.
Lymphocyte cell line (IST-EBV-TW6B) was purchased from the cell bank IRCCS AOU San Martino IST (Italy). Cells were cultured in advanced RPMI 1640 culture medium (Gibco) with 20% of heat inactivated fetal bovine serum (FBS, Gibco), 1% penicillin/streptomycin (P/S, Sigma) and 1% of
Daudi cells (ATCC® CCL213™), originating from a Burkitt’s lymphoma patient, were obtained from American Type Culture Collection (ATCC). Cells were grown in RPMI 1640 culture medium (ATCC) supplemented with 10% of heat inactivated FBS (ATCC), 1% P/S (Sigma) in 75 cm2 not treated cell culture flasks (Corning) with a cell density between 3 × 105–6 cells/mL.
HL60 cells (ATCC® CCL-240™), from an acute myeloid leukemia patient, were purchased from ATCC. They were maintained in Iscove’s Modified Dulbecco’s Medium (Sigma) with 20% heat inactivated FBS (Sigma), 1% Glutamine (Sigma), 1% P/S (Sigma) in 75 cm2 not treated cell culture flasks (Corning), adjusting cell density to 1 × 105–6 cells/mL.