Supernatants from cardiac fibroblasts co-culture with ILC2s or sera from mice were stored at −80°C prior to ELISA. Eotaxin-1 was determined by quantitative sandwich ELISA using Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D Systems) according to manufacturer’s protocols. For anti-myosin IgM ELISA, plates were coated with 0.5 μg/well myosin heavy chain α peptide MyHCα614-62g (Ac-SLKLMATLFSTYASAD; Genscript) and Alkaline Phosphatase AffiniPure goat anti-mouse IgM, μ chain specific (Jackson ImmunoResearch) at a 1:4000 dilution was used to detect anti-myosin IgM in sera.
Myhcα614 62g ac slklmatlfstyasad
Manufactured by GenScript
MyHCα614-62g (Ac-SLKLMATLFSTYASAD) is a synthetic peptide product offered by GenScript. It consists of a 19-amino acid sequence. The core function of this product is to serve as a research tool, without interpretation or extrapolation on its intended use.
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Lab products found in correlation
2 protocols using myhcα614 62g ac slklmatlfstyasad
1
Cardiac Fibroblast-ILC2 Co-culture ELISA
2
Cardiac Fibroblast-ILC2 Co-culture ELISA
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Supernatants from cardiac fibroblasts co-culture with ILC2s or sera from mice were stored at −80°C prior to ELISA. Eotaxin-1 was determined by quantitative sandwich ELISA using Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D Systems) according to manufacturer’s protocols. For anti-myosin IgM ELISA, plates were coated with 0.5 μg/well myosin heavy chain α peptide MyHCα614-62g (Ac-SLKLMATLFSTYASAD; Genscript) and Alkaline Phosphatase AffiniPure goat anti-mouse IgM, μ chain specific (Jackson ImmunoResearch) at a 1:4000 dilution was used to detect anti-myosin IgM in sera.
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